Proteintech

BDNF Monoclonal Antibody (1E5C5)

BDNF Antibody in Immunocytochemistry (ICC/IF)

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BDNF Antibody (66292-1-IG) in ICC/IF

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed PC-12 cells using 66292-1-IG (BDNF antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).

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Product Details

66292-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 21 publications 21 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 4 publications 4 publications

Flow Cytometry (Flow)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Rat, Zebrafish

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

1E5C5

Immunogen

BDNF Fusion Protein Ag11329 (21-247 aa encoded by BC029795)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MKEANIRGQ GGLAYPGVRT HGTLESVNGP KAGSRGLTSL ADTFEHMIEE LLDEDQKVRP NEENNKDADL YTSRVMLSSQ VPLEPPLLFL LEEYKNYLDA ANMSMRVRRH SDPARRGELS VCDSISEWVT AADKKTAVDM SGGTVTVLEK VPVSKGQLKQ YFYETKCNPM GYTKEGCRGI DKRHWNSQCR TTQSYVRALT MDSKKRIGWR FIRIDTSCVC TLTIKRGR (21-247 aa encoded by BC029795 )

Target Information

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

EA participates in pain transition through regulating KCC2 expression by BDNF-TrkB in the spinal cord dorsal horn of male rats.

Neurobiology of pain (Cambridge, Mass.)

Shi M,Zhou J,Hu R,Xu H,Chen Y,Wu X,Chen B,Ma R

Tue Mar 07 00:00:00 EST 2023

EA participates in pain transition through regulating KCC2 expression by BDNF-TrkB in the spinal cord dorsal horn of male rats.

Neurobiology of pain (Cambridge, Mass.)

Shi M,Zhou J,Hu R,Xu H,Chen Y,Wu X,Chen B,Ma R

Tue Mar 07 00:00:00 EST 2023

Tanshinone IIA ameliorates chronic unpredictable mild stress-induced depression-like behavior and cognitive impairment in rats through the BDNF/TrkB/GAT1 signaling pathway.

European journal of pharmacology

Liu SZ,Yang J,Chen LL,Wang P,Lin L

Thu Jan 05 00:00:00 EST 2023

Crocetin confers neuroprotection and is anti-inflammatory in rats with induced glaucoma.

Molecular biology reports

Li Q,Feng P,Lin S,Xu Z,Zhao J,Chen Z,Luo Z,Tao Y,Chen S,Wang P

Wed Feb 01 00:00:00 EST 2023

Baicalein ameliorates Alzheimer's disease via orchestration of CX3CR1/NF-κB pathway in a triple transgenic mouse model.

International immunopharmacology

Xie XM,Hao JJ,Shi JZ,Zhou YF,Liu PF,Wang F,Zheng XM,Yu XY,Wang CC,Yan Y,Du GH,Song JK,He YY,Pang XB

Mon May 01 00:00:00 EDT 2023

Copper Induces Cognitive Impairment in Mice via Modulation of Cuproptosis and CREB Signaling.

Nutrients

Zhang Y,Zhou Q,Lu L,Su Y,Shi W,Zhang H,Liu R,Pu Y,Yin L

Wed Feb 15 00:00:00 EST 2023

Light modulates glucose metabolism by a retina-hypothalamus-brown adipose tissue axis.

Cell

Meng JJ,Shen JW,Li G,Ouyang CJ,Hu JX,Li ZS,Zhao H,Shi YM,Zhang M,Liu R,Chen JT,Ma YQ,Zhao H,Xue T

Thu Jan 19 00:00:00 EST 2023

PPARα is involved in high-fat diet-induced risk avoidance impairment via the regulation of hippocampal BDNF.

Food & function

Zhu XY,Luo J,Wei XJ,Pan LH,Xu XF,Hu LB,Dong YB,Jiang YK,Dong FY,Xu L,Xu LM,Liu AM,Zhang XQ

Mon Jul 17 00:00:00 EDT 2023

Focused Ultrasound Promotes the Delivery of Gastrodin and Enhances the Protective Effect on Dopaminergic Neurons in a Mouse Model of Parkinson's Disease.

Frontiers in cellular neuroscience

Wang Y,Luo K,Li J,Liao Y,Liao C,Chen WS,Chen M,Ao L

Sat Jul 16 00:00:00 EDT 2022

Fucoidan ameliorates LPS-induced neuronal cell damage and cognitive impairment in mice.

International journal of biological macromolecules

Wang Y,Wang Q,Duan L,Li X,Yang W,Huang T,Kong M,Guan F,Ma S

Thu Dec 01 00:00:00 EST 2022

Effect of Qingyangshen glycosides on social defeat mice model.

Journal of ethnopharmacology

Liu D,Wang J,Chang L,Zhu Q,Jiang N,Azhar M,Zeng G

Fri Jul 15 00:00:00 EDT 2022

Perfluorooctane sulfonates induces neurobehavioral changes and increases dopamine neurotransmitter levels in zebrafish larvae.

Chemosphere

Wu L,Dang Y,Liang LX,Gong YC,Zeeshan M,Qian Z,Geiger SD,Vaughn MG,Zhou Y,Li QQ,Chu C,Tan YW,Lin LZ,Liu RQ,Hu LW,Yang BY,Zeng XW,Yu Y,Dong GH

Wed Jun 01 00:00:00 EDT 2022

Rab8A promotes breast cancer progression by increasing surface expression of Tropomyosin-related kinase B.

Cancer letters

Liu Y,Zhang Z,Gao X,Ma Q,Yu Z,Huang S

Wed Jun 01 00:00:00 EDT 2022

Promoted CD4⁺ T cell-derived IFN-γ/IL-10 by photobiomodulation therapy modulates neurogenesis to ameliorate cognitive deficits in APP/PS1 and 3xTg-AD mice.

Journal of neuroinflammation

Wu X,Shen Q,Chang H,Li J,Xing D

Mon Oct 10 00:00:00 EDT 2022

Promoted CD4⁺ T cell-derived IFN-γ/IL-10 by photobiomodulation therapy modulates neurogenesis to ameliorate cognitive deficits in APP/PS1 and 3xTg-AD mice.

Journal of neuroinflammation

Wu X,Shen Q,Chang H,Li J,Xing D

Mon Oct 10 00:00:00 EDT 2022

Dopamine receptor 2 downregulation and brain-derived neurotrophic factor upregulation in the paraventricular nucleus are correlated with brown adipose tissue thermogenesis in rats with bilateral substantia nigra lesions.

Journal of chemical neuroanatomy

Zhang Y,Zhou L,Lian H,Zhang Y,Tong S,Wang Z

Mon Nov 01 00:00:00 EDT 2021

Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway.

Neuropsychiatric disease and treatment

Dong H,Qin YQ,Sun YC,Yao HJ,Cheng XK,Yu Y,Lu SS

Fri Apr 22 00:00:00 EDT 2022

Electroacupuncture Ameliorates Depressive-Like Behaviors in Poststroke Rats via Activating the tPA/BDNF/TrkB Pathway.

Neuropsychiatric disease and treatment

Dong H,Qin YQ,Sun YC,Yao HJ,Cheng XK,Yu Y,Lu SS

Fri Apr 22 00:00:00 EDT 2022

Quercitrin Rapidly Alleviated Depression-like Behaviors in Lipopolysaccharide-Treated Mice: The Involvement of PI3K/AKT/NF-κB Signaling Suppression and CREB/BDNF Signaling Restoration in the Hippocampus.

ACS chemical neuroscience

Sun Y,Zhang H,Wu Z,Yu X,Yin Y,Qian S,Wang Z,Huang J,Wang W,Liu T,Xue W,Chen G

Wed Sep 15 00:00:00 EDT 2021

Methotrexate Neurotoxicity Is Related to Epigenetic Modification of the Myelination Process.

International journal of molecular sciences

Chen YC,Sheen JM,Wang SC,Hsu MH,Hsiao CC,Chang KA,Huang LT

Wed Jun 23 00:00:00 EDT 2021

Colocalization of dopamine receptors in BDNF-expressing peptidergic neurons in the paraventricular nucleus of rats.

Journal of chemical neuroanatomy

Zhou L,Zhang Y,Lian H,Li Y,Wang Z

Wed Jul 01 00:00:00 EDT 2020

Electroacupuncture promotes axonal regrowth by attenuating the myelin-associated inhibitors-induced RhoA/ROCK pathway in cerebral ischemia/reperfusion rats.

Brain research

Chen S,Wang H,Xu H,Zhang Y,Sun H

Tue Dec 01 00:00:00 EST 2020

GABA-mediated activated microglia induce neuroinflammation in the hippocampus of mice following cold exposure through the NLRP3 inflammasome and NF-κB signaling pathways.

International immunopharmacology

Lang L,Xu B,Yuan J,Li S,Lian S,Chen Y,Guo J,Yang H

Tue Dec 01 00:00:00 EST 2020

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Behavioural brain research

Lian S,Xu B,Wang D,Wang L,Li W,Yao R,Ji H,Wang J,Guo J,Li S,Yang H

Fri Feb 01 00:00:00 EST 2019

Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats.

Behavioural brain research

Lian S,Xu B,Wang D,Wang L,Li W,Yao R,Ji H,Wang J,Guo J,Li S,Yang H

Fri Feb 01 00:00:00 EST 2019

Response of the maternal hypothalamus to cold stress during late pregnancy in rats.

Brain research

Wang D,Xu B,Wang J,Wang H,Guo J,Ji H,Li S,Wu R,Yang H,Lian S

Fri Nov 01 00:00:00 EDT 2019

Activation of BDNF-TrkB signaling pathway-regulated brain inflammation in pentylenetetrazole-induced seizures in zebrafish.

Fish & shellfish immunology

Jin M,Sheng W,Han L,He Q,Ji X,Liu K

Sat Dec 01 00:00:00 EST 2018

Activation of BDNF-TrkB signaling pathway-regulated brain inflammation in pentylenetetrazole-induced seizures in zebrafish.

Fish & shellfish immunology

Jin M,Sheng W,Han L,He Q,Ji X,Liu K

Sat Dec 01 00:00:00 EST 2018

Radix Scutellariae Attenuates CUMS-Induced Depressive-Like Behavior by Promoting Neurogenesis via cAMP/PKA Pathway.

Neurochemical research

Zhang R,Guo L,Ji Z,Li X,Zhang C,Ma Z,Fu Q,Qu R,Ma S

Thu Nov 01 00:00:00 EDT 2018

Radix Scutellariae Attenuates CUMS-Induced Depressive-Like Behavior by Promoting Neurogenesis via cAMP/PKA Pathway.

Neurochemical research

Zhang R,Guo L,Ji Z,Li X,Zhang C,Ma Z,Fu Q,Qu R,Ma S

Thu Nov 01 00:00:00 EDT 2018

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Behavioural brain research

Lian S,Wang D,Xu B,Guo W,Wang L,Li W,Ji H,Wang J,Kong F,Zhen L,Li S,Zhang L,Guo J,Yang H

Mon Jul 02 00:00:00 EDT 2018

Prenatal cold stress: Effect on maternal hippocampus and offspring behavior in rats.

Behavioural brain research

Lian S,Wang D,Xu B,Guo W,Wang L,Li W,Ji H,Wang J,Kong F,Zhen L,Li S,Zhang L,Guo J,Yang H

Mon Jul 02 00:00:00 EDT 2018

Influencing mechanism of magnolol on expression of BDNF and Bax in rats with cerebral ischemic stroke.

Experimental and therapeutic medicine

Liu Z,Xie J,Lin K,Qi L

Sat Dec 01 00:00:00 EST 2018

Influencing mechanism of magnolol on expression of BDNF and Bax in rats with cerebral ischemic stroke.

Experimental and therapeutic medicine

Liu Z,Xie J,Lin K,Qi L

Sat Dec 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Abrineurin; BDNF; Brain-derived neurotrophic factor; H-BDNF; neurotrophin

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Gene Aliases: ANON2; BDNF; BULN2

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UniProt ID: (Human) Q9BYY7

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Entrez Gene ID: (Human) 627

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Function(s)

neurotrophin TRKB receptor binding growth factor activity neurotrophic factor

Process(es)

cell-cell signaling nervous system development axon guidance synapse assembly brain-derived neurotrophic factor receptor signaling pathway positive regulation of brain-derived neurotrophic factor receptor signaling pathway negative regulation of neuron apoptotic process collateral sprouting positive regulation of collateral sprouting positive regulation of synapse assembly

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BDNF Monoclonal Antibody (1E5C5)

BDNF Antibody (66292-1-IG) in ICC/IF

Product Details

66292-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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