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Product Details
A300-216A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 825 and the C-terminus (residue 886) of human FAD-binding protein BRAF35-HDAC complex 110 kDa (lysine-specific histone demethylase 1).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Mouse
Target Information
LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A; amine oxidase (flavin containing) domain 2; BRAF35-HDAC complex protein BHC110; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; Flavin-containing amine oxidase domain-containing protein 2; lysine (K)-specific demethylase 1A; lysine-specific histone demethylase 1; Lysine-specific histone demethylase 1A; RP1-184J9.1
Gene Aliases: AOF2; BHC110; CPRF; KDM1; KDM1A; KIAA0601; LSD1
UniProt ID: (Human) O60341
Entrez Gene ID: (Human) 23028
RNA polymerase II transcription factor binding
p53 binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
histone deacetylase activity
protein binding
transcription factor binding
oxidoreductase activity
enzyme binding
ligand-dependent nuclear receptor transcription coactivator activity
demethylase activity
histone demethylase activity
histone demethylase activity (H3-K4 specific)
histone demethylase activity (H3-K9 specific)
histone demethylase activity (H3-dimethyl-K4 specific)
telomeric DNA binding
MRF binding
transcription regulatory region DNA binding
flavin adenine dinucleotide binding
androgen receptor binding
telomeric repeat-containing RNA binding
oxidase
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein demethylation
blood coagulation
cell proliferation
regulation of double-strand break repair via homologous recombination
regulation of primitive erythrocyte differentiation
histone deacetylation
pituitary gland development
granulocyte differentiation
negative regulation of protein binding
histone H3-K9 demethylation
positive regulation of histone ubiquitination
cellular response to UV
histone H3-K4 demethylation
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of erythrocyte differentiation
positive regulation of megakaryocyte differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hormone biosynthetic process
positive regulation of sequence-specific DNA binding transcription factor activity
negative regulation of histone H3-K4 methylation
negative regulation of histone H3-K9 methylation
muscle cell development
oxidation-reduction process
cellular response to gamma radiation
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of cellular protein localization
positive regulation of neural precursor cell proliferation
positive regulation of stem cell proliferation
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