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BHLHE41 Antibody (PA5-99229) in ICC/IF
Immunocytochemistry analysis of BHLHE41 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with BHLHE41 polyclonal antibody (Product # PA5-99229) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-99229
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human BHLHE41(Accession Q9C0J9), corresponding to amino acid residues R11-N61.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2818162
Product Specific Information
Antibody detects endogenous levels of total BHLHB3.
Target Information
BHLHE41 may be a transcriptional repressor that represses both basal and activated transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-Dec; basic helix-loop-helix domain containing, class B, 3; basic helix-loop-helix domain containing, class B3; basic helix-loop-helix family, member e41; bHLH transcriptional factor Dec2; bHLHb3; bHLHe41; Class B basic helix-loop-helix protein 3; Class E basic helix-loop-helix protein 41; Differentially expressed in chondrocytes protein 2; Enhancer-of-split and hairy-related protein 1; hDEC2; mDEC2; SHARP-1
Gene Aliases: 6430520M22Rik; Bhlhb2l; BHLHB3; BHLHE41; DEC2; hDEC2; SHARP-1; SHARP1
UniProt ID: (Human) Q9C0J9, (Mouse) Q99PV5
Entrez Gene ID: (Human) 79365, (Rat) 117095, (Mouse) 79362
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
transcriptional repressor activity, RNA polymerase II transcription factor binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription corepressor activity
protein binding
protein homodimerization activity
histone deacetylase binding
bHLH transcription factor binding
MRF binding
protein heterodimerization activity
E-box binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
protein dimerization activity
N-box binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
cell proliferation
organ morphogenesis
negative regulation of myotube differentiation
negative regulation of transcription by competitive promoter binding
cell differentiation
circadian regulation of gene expression
negative regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
nervous system development
circadian rhythm
entrainment of circadian clock
regulation of neuronal synaptic plasticity
regulation of transcription, DNA-templated
rhythmic process
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