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Invitrogen
BHLHE41 Recombinant Rabbit Monoclonal Antibody (1H18L6)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
BHLHE41 Antibody (702917) in WB
Knockdown of BHLH3 was achieved by transfecting MDA-MB-231 cells with BHLH3 specific siRNA (Silencer® select Product # s35755). Western blot analysis (Fig a) was performed using modified whole cell extracts (1% SDS) from BHLH3 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Anti-BHLH3 Recombinant Rabbit Monoclonal Antibody (Product # 702917, 1:1000 dilution) and Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Prod... View More
Product Details
702917
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H18L6
Immunogen
Protein corresponding to human BHLH3 [aa246-aa482]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2784551
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Cat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
BHLHE41 may be a transcriptional repressor that represses both basal and activated transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2-Dec; basic helix-loop-helix domain containing, class B, 3; basic helix-loop-helix family, member e41; bHLHb3; bHLHe41; Class B basic helix-loop-helix protein 3; Class E basic helix-loop-helix protein 41; Differentially expressed in chondrocytes protein 2; Enhancer-of-split and hairy-related protein 1; hDEC2; SHARP-1
Gene Aliases: BHLHB3; BHLHE41; DEC2; hDEC2; SHARP1
UniProt ID: (Human) Q9C0J9
Entrez Gene ID: (Human) 79365
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
transcriptional repressor activity, RNA polymerase II transcription factor binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription corepressor activity
protein binding
protein homodimerization activity
histone deacetylase binding
bHLH transcription factor binding
MRF binding
protein heterodimerization activity
E-box binding
basic helix-loop-helix transcription factor
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
cell proliferation
organ morphogenesis
negative regulation of myotube differentiation
negative regulation of transcription by competitive promoter binding
cell differentiation
circadian regulation of gene expression
negative regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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