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Invitrogen
BID Polyclonal Antibody
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
BID Antibody (PA5-29159) in ICC/IF
BID Polyclonal Antibody detects BID protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37°C for 30 min. Green: BID protein stained by BID Polyclonal Antibody (Product # PA5-29159) diluted at 1:500. Blue: Hoechst 33343 staining.
Product Details
PA5-29159
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human Bid Recombinant protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546635
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. Bid is a member of the BCL-2 family of cell death regulators. BID is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. BID is also a pro-apoptotic member of the Bcl 2 family. BID usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: apoptic d; apoptic death agonist; apoptotic death agonist BID; BH3-interacting domain death agonist; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; desmocollin type 4; HGNC:1050; Human BID coding sequence; MGC15319; MGC42355; OTTHUMP00000196197; p22 BID
Gene Aliases: 2700049M22Rik; AI875481; AU022477; BID; FP497
UniProt ID: (Human) P55957, (Rat) Q9JLT6, (Mouse) P70444
Entrez Gene ID: (Human) 637, (Rat) 64625, (Mouse) 12122
release of cytochrome c from mitochondria
protein targeting to mitochondrion
activation of cysteine-type endopeptidase activity involved in apoptotic process
brain development
apoptotic mitochondrial changes
response to estradiol
regulation of protein oligomerization
positive regulation of protein oligomerization
positive regulation of protein homooligomerization
glial cell apoptotic process
regulation of cell proliferation
positive regulation of apoptotic process
protein homooligomerization
establishment of protein localization to membrane
positive regulation of release of cytochrome c from mitochondria
extrinsic apoptotic signaling pathway
hepatocyte apoptotic process
mitochondrial outer membrane permeabilization
regulation of mitochondrial membrane permeability involved in apoptotic process
regulation of G1/S transition of mitotic cell cycle
positive regulation of extrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway via death domain receptors
signal transduction in response to DNA damage
regulation of apoptotic process
neuron apoptotic process
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
apoptotic process
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