Invitrogen

BMAL1 Polyclonal Antibody

2 Published Figures

7 References

View all (31) BMAL1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)
Published Figures (2)

BMAL1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

BMAL1 Antibody (PA1-523) in ICC/IF

Immunofluorescent analysis of BMAL1 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with BMAL1 Rabbit Polyclonal Antibody (Product # PA1-523) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # ... View More

BMAL1 Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F))

BMAL1 Antibody in Immunohistochemistry (IHC)

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Product Details

PA1-523

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Hamster, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C D(582) M I D N D Q G S S S P S(594)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2059614

Product Specific Information

PA1-523 detects BMAL1/aryl hydrocarbon nuclear translocator 3 (ARNT3) from hamster and mouse tissues as well as recombinant human BMAL1.

PA1-523 has been sucessfully used in Western blot procedures. By Western blot, this antibody detects a 110 kDa protein which corresponds to the product of a hamster GST-BMAL1 fusion construct overexpressed in E. coli. This antibody detects a non-specific band in U87-MG cell lysates at ~105kDa and in NIH-3T3 cell lysates at ~135kDa.

PA1-523 immunizing peptide corresponds to amino acid residues 582-594 from mouse BMAL1. This sequence is completely conserved between mouse, rat, guinea pig, and human BMAL1. PA1-523 immunizing peptide (Cat. # PEP-075) is available for use in neutralization and control experiments.

Target Information

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prolonged Administration of Melatonin Ameliorates Liver Phenotypes in Cholestatic Murine Model.

Cellular and molecular gastroenterology and hepatology

Ceci L,Chen L,Baiocchi L,Wu N,Kennedy L,Carpino G,Kyritsi K,Zhou T,Owen T,Kundu D,Sybenga A,Isidan A,Ekser B,Franchitto A,Onori P,Gaudio E,Mancinelli R,Francis H,Alpini G,Glaser S

Published figure using BMAL1 polyclonal antibody (Product # PA1-523) in Immunohistochemistry (Frozen)

Mon Sep 26 00:00:00 EDT 2022

Prolonged Administration of Melatonin Ameliorates Liver Phenotypes in Cholestatic Murine Model.

Cellular and molecular gastroenterology and hepatology

Ceci L,Chen L,Baiocchi L,Wu N,Kennedy L,Carpino G,Kyritsi K,Zhou T,Owen T,Kundu D,Sybenga A,Isidan A,Ekser B,Franchitto A,Onori P,Gaudio E,Mancinelli R,Francis H,Alpini G,Glaser S

Published figure using BMAL1 polyclonal antibody (Product # PA1-523) in Immunohistochemistry (Frozen)

Mon Sep 26 00:00:00 EDT 2022

Melatonin modulates daytime-dependent synaptic plasticity and learning efficiency.

Journal of pineal research

Jilg A,Bechstein P,Saade A,Dick M,Li TX,Tosini G,Rami A,Zemmar A,Stehle JH

PA1-523 was used in Immunohistochemistry to investigate the role of the neurohormone melatonin as a circadian time cue for hippocampal signaling and memory formation.

Mon Apr 01 00:00:00 EDT 2019

Dysregulated circadian rhythm pathway in human osteoarthritis: NR1D1 and BMAL1 suppression alters TGF-β signaling in chondrocytes.

Osteoarthritis and cartilage

Akagi R,Akatsu Y,Fisch KM,Alvarez-Garcia O,Teramura T,Muramatsu Y,Saito M,Sasho T,Su AI,Lotz MK

PA1523 was used in immunohistochemistry to study circadian rhythmicity in cultured chondrocytes and determine the role of NR1D1 and BMAL1 in regulating chondrocyte functions

Thu Jun 01 00:00:00 EDT 2017

The transcription factor Runx2 is under circadian control in the suprachiasmatic nucleus and functions in the control of rhythmic behavior.

PloS one

Reale ME,Webb IC,Wang X,Baltazar RM,Coolen LM,Lehman MN

PA1-523 was used in western blot to study the circadian control of Runx2 expression in the suprachiasmatic nucleus and its role in regulating circadian behavioural rhythms

Mon Jul 22 00:00:00 EDT 2013

Expression of circadian rhythm genes in gonadotropin-releasing hormone-secreting GT1-7 neurons.

Endocrinology

Gillespie JM,Chan BP,Roy D,Cai F,Belsham DD

PA1-523 was used in western blot to investigate the role of the clock machinery in GnRH neuronal function.

Mon Dec 01 00:00:00 EST 2003

Circadian Transcription. Thinking outside the E-Box.

The Journal of biological chemistry

Muñoz E,Brewer M,Baler R

PA1-523 was used in immunocytochemistry to study the role of E-Box in the pathway of circadian clock.

Fri Sep 27 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: ARNT-like protein 1, brain and muscle; Arnt3; aryl hydrocarbon receptor nuclear translocator-like; Aryl hydrocarbon receptor nuclear translocator-like protein 1; Basic helix-loop-helix ARNT-like protein 1; basic-helix-loop-helix-PAS orphan MOP3; Basic-helix-loop-helix-PAS protein MOP3; bHLH-PAS protein JAP3; bHLHe5; Brain and muscle ARNT-like 1; CG8727 PA; Class E basic helix-loop-helix protein 5; cycle; Member of PAS protein 3; member of PAS superfamily 3; MGC47515; PAS domain-containing protein 3; testis tissue sperm-binding protein Li 50e; Tic

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Gene Aliases: Arnt3; ARNTL; BHLHE5; BMAL1; BMAL1b; bmal1b'; BMAL1c; JAP3; MOP3; PASD3; TIC

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UniProt ID: (Human) O00327, (Rat) Q9EPW1, (Mouse) Q9WTL8

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Entrez Gene ID: (Human) 406, (Chinese hamster) 100755141, (Rat) 29657, (Mouse) 11865

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Function(s)

transcription regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding core promoter sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II transcription factor binding DNA binding protein binding aryl hydrocarbon receptor binding bHLH transcription factor binding sequence-specific DNA binding protein heterodimerization activity Hsp90 protein binding repressing transcription factor binding E-box binding transcription factor activity, sequence-specific DNA binding signal transducer activity protein dimerization activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor binding basic helix-loop-helix transcription factor

Process(es)

protein import into nucleus, translocation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter spermatogenesis circadian rhythm negative regulation of TOR signaling circadian regulation of gene expression regulation of protein catabolic process regulation of hair cycle positive regulation of circadian rhythm proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of fat cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis regulation of insulin secretion regulation of cell cycle response to redox state maternal process involved in parturition positive regulation of canonical Wnt signaling pathway oxidative stress-induced premature senescence regulation of type B pancreatic cell development negative regulation of glucocorticoid receptor signaling pathway regulation of cellular senescence positive regulation of skeletal muscle cell differentiation transcription, DNA-templated signal transduction regulation of circadian rhythm rhythmic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BMAL1 Polyclonal Antibody

BMAL1 Antibody (PA1-523) in ICC/IF

Product Details

PA1-523

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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