Invitrogen

BMP-2 Polyclonal Antibody, PeproTech®

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BMP-2 Antibody (500-P195-1MG) in WB

Western Blot: To detect Human/Murine/Rat BMP-2 by Western Blot analysis BMP-2 Polyclonal Antibody (Product # 500-P195-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human/Murine/Rat BMP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Product Details

500-P195-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 4 publications 4 publications

ELISA (ELISA)

0.5-2.0 µg/mL

Neutralization (Neu)

View 2 publications 2 publications

In vitro Assay (IV)

View 3 publications 3 publications

Immunostaining (IS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bacteria, Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human/Murine/Rat BMP-2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929769

Product Specific Information

AA Sequence of recombinant protein: MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BMP-2. Anti-Human/Murine/Rat BMP-2-specific antibody was purified by affinity chromatography employing immobilized Human/Murine/Rat BMP-2 matrix.

Sandwich ELISA: To detect Human/Murine/Rat BMP-2 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human/Murine/Rat BMP-2 (500-P195Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human/Murine/Rat BMP-2.

Western Blot: To detect Human/Murine/Rat BMP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human/Murine/Rat BMP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Bone Morphogenic Proteins (BMP) are members of the TGF-beta superfamily that affect bone and cartilage formation (Hogan 1996, Reddi 1998 and Francis-West et al. 1999). Mature BMPs are 30-38 kDa proteins that assume a TGF-beta -like cysteine knot configuration. Lovostatin increases bone formation by turning on the bmp-2 gene (Mundy et al. 1999). BMPs stimulate the production of specific bone matrix proteins and alter stromal cell and osteoclast proliferation (Macias et al. 1999, Lecanda et al. 1997). BMPs may also be an important factor for development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis (Hogan 1996, Dale and Wardle 1999). BMPs appear to be responsible for normal dorsal/ventral patterning. Like TGF-beta, BMPs bind to a type II receptor, which then recruits the transducing type I receptor unit, activating the Smad protein signaling pathway (Massague 1994, Derynck 1997, Attisano 1993).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Bone Morphogenetic Protein 2 (BMP-2) Aggregates Can be Solubilized by Albumin-Investigation of BMP-2 Aggregation by Light Scattering and Electrophoresis.

Pharmaceutics

Sundermann J,Zagst H,Kuntsche J,Wätzig H,Bunjes H

500-P195 was used in Western Blotting to study the solubility behavior of bone morphogenetic protein 2 (BMP-2).

Wed Nov 25 00:00:00 EST 2020

Bone Morphogenetic Protein 2 (BMP-2) Aggregates Can be Solubilized by Albumin-Investigation of BMP-2 Aggregation by Light Scattering and Electrophoresis.

Pharmaceutics

Sundermann J,Zagst H,Kuntsche J,Wätzig H,Bunjes H

500-P195 was used in Western Blotting to study the solubility behavior of bone morphogenetic protein 2 (BMP-2).

Wed Nov 25 00:00:00 EST 2020

Deregulated AUF1 Assists BMP-EZH2-Mediated Delayed Wound Healing during Candida albicans Infection.

Journal of immunology (Baltimore, Md. : 1950)

Mahadik K,Yadav P,Bhatt B,Shah RA,Balaji KN

Sat Dec 15 00:00:00 EST 2018

Deregulated AUF1 Assists BMP-EZH2-Mediated Delayed Wound Healing during Candida albicans Infection.

Journal of immunology (Baltimore, Md. : 1950)

Mahadik K,Yadav P,Bhatt B,Shah RA,Balaji KN

Sat Dec 15 00:00:00 EST 2018

Decidualisation and placentation defects are a major cause of age-related reproductive decline.

Nature communications

Woods L,Perez-Garcia V,Kieckbusch J,Wang X,DeMayo F,Colucci F,Hemberger M

500-P195 was used in Western Blotting to investigate decreasing mammalian reproductive performance with age in mice due to hormone responsiveness of the uterus.

Tue Sep 05 00:00:00 EDT 2017

Decidualisation and placentation defects are a major cause of age-related reproductive decline.

Nature communications

Woods L,Perez-Garcia V,Kieckbusch J,Wang X,DeMayo F,Colucci F,Hemberger M

500-P195 was used in Western Blotting to investigate decreasing mammalian reproductive performance with age in mice due to hormone responsiveness of the uterus.

Tue Sep 05 00:00:00 EDT 2017

Hypoxia and Reactive Oxygen Species Homeostasis in Mesenchymal Progenitor Cells Define a Molecular Mechanism for Fracture Nonunion.

Stem cells (Dayton, Ohio)

Muinos-López E,Ripalda-Cemboráin P,López-Martínez T,González-Gil AB,Lamo-Espinosa JM,Valentí A,Mortlock DP,Valentí JR,Prósper F,Granero-Moltó F

Thu Sep 01 00:00:00 EDT 2016

Hypoxia and Reactive Oxygen Species Homeostasis in Mesenchymal Progenitor Cells Define a Molecular Mechanism for Fracture Nonunion.

Stem cells (Dayton, Ohio)

Muinos-López E,Ripalda-Cemboráin P,López-Martínez T,González-Gil AB,Lamo-Espinosa JM,Valentí A,Mortlock DP,Valentí JR,Prósper F,Granero-Moltó F

Thu Sep 01 00:00:00 EDT 2016

Effective Inhibition of Bone Morphogenetic Protein Function by Highly Specific Llama-Derived Antibodies.

Molecular cancer therapeutics

Calpe S,Wagner K,El Khattabi M,Rutten L,Zimberlin C,Dolk E,Verrips CT,Medema JP,Spits H,Krishnadath KK

500-P195 was used in Western Blotting to generate three types of llama-derived heavy chain variable domains (VHH) that selectively bind to either bone morphogenetic protein (BMP)4, to BMP2 and 4, or to BMP2, 4, 5, and 6.

Sun Nov 01 00:00:00 EDT 2015

Bmp7 regulates the survival, proliferation, and neurogenic properties of neural progenitor cells during corticogenesis in the mouse.

PloS one

Segklia A,Seuntjens E,Elkouris M,Tsalavos S,Stappers E,Mitsiadis TA,Huylebroeck D,Remboutsika E,Graf D

Fri Jun 29 00:00:00 EDT 2012

Low-power GaAlAs laser irradiation promotes the proliferation and osteogenic differentiation of stem cells via IGF1 and BMP2.

PloS one

Wu JY,Wang YH,Wang GJ,Ho ML,Wang CZ,Yeh ML,Chen CH

Tue Jan 22 00:00:00 EST 2013

Involvement of twisted gastrulation in T cell-independent plasma cell production.

Journal of immunology (Baltimore, Md. : 1950)

Tsalavos S,Segklia K,Passa O,Petryk A,O'Connor MB,Graf D

Wed Jun 15 00:00:00 EDT 2011

Involvement of twisted gastrulation in T cell-independent plasma cell production.

Journal of immunology (Baltimore, Md. : 1950)

Tsalavos S,Segklia K,Passa O,Petryk A,O'Connor MB,Graf D

Wed Jun 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 2610024H22Rik; AL117858; AW546137; BB189135; BMP; BMP-2; BMP-2A; BMPR-II; BMPRII; Bone morphogenetic protein; Bone morphogenetic protein 2; Bone morphogenetic protein 2A; H-BMP-2

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Gene Aliases: AI467020; BDA2; Bmp-2; BMP2; BMP2A

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UniProt ID: (Human) P12643, (Mouse) P21274, (Rat) P49001

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Entrez Gene ID: (Human) 650, (Mouse) 12156, (Rat) 29373

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Function(s)

retinol dehydrogenase activity receptor binding cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity phosphatase activator activity co-receptor binding SMAD binding protein heterodimerization activity BMP receptor binding protein domain specific binding protein homodimerization activity growth factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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BMP-2 Polyclonal Antibody, PeproTech®

BMP-2 Antibody (500-P195-1MG) in WB

Product Details

500-P195-1MG

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

In vitro Assay (IV)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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