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BNIP3 Antibody (PA5-78881) in WB
Western blot analysis of BNIP3 in Lane 1: rat brain tissue lysate, Lane 2: rat kidney tissue lysate, Lane 3: rat heart tissue lysate, Lane 4: rat testis tissue lysate, Lane 5: mouse brain tissue lysate, Lane 6: mouse kidney tissue lysate, Lane 7: mouse heart tissue lysate, Lane 8: mouse testis tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minute... View More
Product Details
PA5-78881
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human BNIP3 (KEFLFKHPKRTATLSMRNTSVMKK).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2745997
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19 kDa-interacting protein 1, NIP3; BCL2/adenovirus E1B 19 kDa-interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3; BCL2/adenovirus E1B 19kDa-interacting protein 1, NIP3; BCL2/adenovirus E1B interacting protein 1, NIP3
Gene Aliases: BNIP3; NIP3
UniProt ID: (Human) Q12983, (Mouse) O55003
Entrez Gene ID: (Human) 664, (Rat) 84480, (Mouse) 12176
response to hypoxia
apoptotic process
cell death
granzyme-mediated apoptotic signaling pathway
negative regulation of mitochondrial fusion
mitochondrial protein catabolic process
positive regulation of apoptotic process
positive regulation of programmed cell death
positive regulation of protein complex disassembly
mitochondrial fragmentation involved in apoptotic process
negative regulation of membrane potential
regulation of mitochondrial membrane permeability
autophagic cell death
brown fat cell differentiation
neuron apoptotic process
defense response to virus
response to hyperoxia
cellular response to hydrogen peroxide
cellular response to mechanical stimulus
cellular response to cobalt ion
cellular response to hypoxia
reactive oxygen species metabolic process
positive regulation of mitochondrial fission
positive regulation of release of cytochrome c from mitochondria
intrinsic apoptotic signaling pathway
mitochondrial outer membrane permeabilization
toxin transport
intrinsic apoptotic signaling pathway in response to hypoxia
regulation of mitochondrion organization
negative regulation of apoptotic process
regulation of aerobic respiration
negative regulation of reactive oxygen species metabolic process
mitophagy
positive regulation of autophagy
positive regulation of cardiac muscle cell apoptotic process
positive regulation of macroautophagy
negative regulation of cell death
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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