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Invitrogen
BNIP3 Recombinant Rabbit Monoclonal Antibody (8H14L2)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
BNIP3 Antibody (701696) in ICC/IF
Immunofluorescence was performed on methanol fixed Hela cells treated with Cobalt Chloride (15 µm/ 48 hours), for detection of BNIP3 using Anti-BNIP3 Recombinant Rabbit Monoclonal Antibody (Product # 701696, 1-2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of BNIP3 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountan... View More
Product Details
701696
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
8H14L2
Immunogen
Protein corresponding to Human BNIP3 (aa 1-140)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2607981
Product Specific Information
This antibody is predicted to react with Monkey, Bovine and Equine.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3
Gene Aliases: BNIP3; NIP3
UniProt ID: (Human) Q12983
Entrez Gene ID: (Human) 664
mitophagy
response to hypoxia
apoptotic process
cell death
granzyme-mediated apoptotic signaling pathway
positive regulation of autophagy
negative regulation of mitochondrial fusion
positive regulation of cardiac muscle cell apoptotic process
positive regulation of macroautophagy
mitochondrial protein catabolic process
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of programmed cell death
positive regulation of protein complex disassembly
mitochondrial fragmentation involved in apoptotic process
negative regulation of membrane potential
regulation of mitochondrial membrane permeability
autophagic cell death
brown fat cell differentiation
neuron apoptotic process
defense response to virus
response to hyperoxia
negative regulation of cell death
cellular response to hydrogen peroxide
cellular response to mechanical stimulus
cellular response to cobalt ion
cellular response to hypoxia
reactive oxygen species metabolic process
positive regulation of mitochondrial fission
positive regulation of release of cytochrome c from mitochondria
mitochondrial outer membrane permeabilization
toxin transport
regulation of aerobic respiration
intrinsic apoptotic signaling pathway in response to hypoxia
negative regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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