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Invitrogen
BNIP3 Monoclonal Antibody (ANa40)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
BNIP3 Antibody (MA1-24688) in WB
Western blot was performed using Anti-BNIP3 Monoclonal Antibody (ANa40)(Product # MA1-24688) and a 28kDa band corresponding to BNIP3 was observed across cell lines tested. Membrane enriched extracts (30ug µg lysate) of HeLa (Lane 1), HeLa treated with Cobalt Chloride, 150uM,48Hrs (Lane 2), MCF7 (Lane 3), MCF7 treated with Cobalt Chloride,150uM,48Hrs (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot®... View More
Product Details
MA1-24688
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
ANa40
Immunogen
Recombinant fragment corresponding to residues 1-163 of human BNIP3.
Conjugate
Form
Liquid
Concentration
2.4 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2066655
Product Specific Information
Positive Control: 293T cells transfected with BNIP3.
Target Information
NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BCL2/adenovirus E1B 19kDa interacting protein 3
Gene Aliases: BNIP3; NIP3
UniProt ID: (Human) Q12983
Entrez Gene ID: (Human) 664
mitophagy
response to hypoxia
apoptotic process
cell death
granzyme-mediated apoptotic signaling pathway
positive regulation of autophagy
negative regulation of mitochondrial fusion
positive regulation of cardiac muscle cell apoptotic process
positive regulation of macroautophagy
mitochondrial protein catabolic process
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of programmed cell death
positive regulation of protein complex disassembly
mitochondrial fragmentation involved in apoptotic process
negative regulation of membrane potential
regulation of mitochondrial membrane permeability
autophagic cell death
brown fat cell differentiation
neuron apoptotic process
defense response to virus
response to hyperoxia
negative regulation of cell death
cellular response to hydrogen peroxide
cellular response to mechanical stimulus
cellular response to cobalt ion
cellular response to hypoxia
reactive oxygen species metabolic process
positive regulation of mitochondrial fission
positive regulation of release of cytochrome c from mitochondria
mitochondrial outer membrane permeabilization
toxin transport
regulation of aerobic respiration
intrinsic apoptotic signaling pathway in response to hypoxia
negative regulation of reactive oxygen species metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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