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BNP Antibody (MA5-51438) in WB
Western blot analysis of BNP in various lysates. Samples were incubated with BNP Recombinant monoclonal antibody (Product # MA5-51438) using a dilution of 1:1,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-51438
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6Q3R9
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 35-134 of human BNP (NP_002512.1).
Conjugate
Form
Liquid
Concentration
1.4 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_3094331
Product Specific Information
Immunogen sequence: ASDLETSGLQ EQRNHLQGKL SELQVEQTSL EPLQESPRPT GVWKSREVAT EGIRGHRKMV LYTLRAPRSP KMVQGSGCFG RKMDRISSSS GLGCKVLRRH
Target Information
This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BNP-32; Brain natriuretic factor; Brain natriuretic factor prohormone; brain natriuretic peptide; brain type natriuretic peptide; Gamma-brain natriuretic peptide; Iso-ANP; natriuretic peptide precursor B; natriuretic peptide precursor type B; Natriuretic peptides B; natriuretic protein; preproBNP; type-B natriuretic factor
Gene Aliases: AA408272; BNF; BNP; NPPB
UniProt ID: (Human) P16860, (Rat) P13205, (Mouse) P40753
Entrez Gene ID: (Human) 4879, (Rat) 25105, (Mouse) 18158
response to hypoxia
negative regulation of systemic arterial blood pressure
cGMP biosynthetic process
inflammatory response
receptor guanylyl cyclase signaling pathway
heart development
regulation of blood pressure
response to organic cyclic compound
negative regulation of cell growth
response to lipopolysaccharide
positive regulation of urine volume
positive regulation of renal sodium excretion
response to drug
response to peptide hormone
negative regulation of smooth muscle cell proliferation
regulation of blood vessel size
response to calcium ion
cellular response to mechanical stimulus
positive regulation of collecting lymphatic vessel constriction
cell surface receptor signaling pathway
body fluid secretion
negative regulation of angiogenesis
regulation of vasodilation
regulation of vascular permeability
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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