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T7-Tag Antibody (BSM-33012M) in WB
T7-Tagged Fusion Protein Overexpression E.coli Lysates probed with T7-tag Monoclonal Antibody, Unconjugated (bsm-33012M ) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Product Details
BSM-33012M
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Tag
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
3G7
Immunogen
KLH conjugated synthetic peptide of T7-tag:MASMTGGQQMG
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 50% glycerol, 1% BSA
Contains
0.03% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Target Information
The T7 Tag is an 11 amino acid peptide derived from the bacteriophage T7 major capsid protein, gene 10. T7 cleavage site: MASMTGGQQMG. Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MASMTGGQQMG; T7 cleavage
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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