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Invitrogen
BTLA Chimeric Recombinant Rabbit Monoclonal Antibody (HMBT-6B2)
FIGURE: 1 / 1
BTLA Chimeric Antibody (MA5-48131) in ELISA
ELISA analysis of BTLA chimeric monoclonal antibody (Product # MA5-48131) (red line) and isotype control (blue line) was performed by coating plate with BTLA (CD272)-Fc fusion protein at a concentration of 5 µg/mL. A 3-fold serial dilution from 10,000 to 0.17 ng/mL of the antibody was done. For signal detection, a 1:4,000 dilution of anti-rabbit IgG1 antibody was used. The second increase in absorbance at 3333 ng/mL is suggestive of a possible secondary binding epitope.
Product Details
MA5-48131
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HMBT-6B2
Immunogen
This antibody was raised by immunising Armenian hamsters with mouse BTLA:Fc fusion protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074574
Product Specific Information
Specificity: This antibody is specific for B and T lymphocyte attenuator (BTLA), which functions as a negative regulator of T cell activation and proliferation, and attenuates B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).
Target Information
BTLA or B and T-lymphocyte attenuator is a member of the co-inhibitory receptors of the CD28 superfamily. BTLA is a lymphocyte inhibitory receptor which inhibits lymphocytes during immunes responses. BTLA is constitutively expressed on most CD4+ and CD8+ T cells and its expression progressively decreases upon T cell activation. It remains expressed on Th1 cells, but not Th2 cells. BTLA is a unique co-receptor that interacts with the tumor necrosis factor receptor superfamily member herpesvirus entry mediator (HVEM). This interation is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B and T lymphocyte associated protein; B and T lymphocyte attenuator; B- and T-lymphocyte attenuator; B- and T-lymphocyte-associated protein; BTLA_HUMAN; CD272; CD272 antigen; FLJ16065; MGC129743
Gene Aliases: A630002H24; Btla
UniProt ID: (Mouse) Q7TSA3
Entrez Gene ID: (Mouse) 208154
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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