Invitrogen

BTRC Polyclonal Antibody

View all (25) BTRC antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-27756

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 93 and 384 of Human beta-TrCP
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545232

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (99%), Rat (98%), Xenopus laevis (96%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: beta-transducin repeat containing protein; beta-TrCP1; E3RSIkappaB; Epididymis tissue protein Li 2a; F-box and WD repeats protein beta-TrCP; F-box and WD-repeat protein 1B; F-box/WD repeat-containing protein 1A; pIkappaBalpha-E3 receptor subunit

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Gene Aliases: BETA-TRCP; beta-TrCP1; betaTrCP; BTRC; BTRCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1

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UniProt ID: (Human) Q9Y297

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Entrez Gene ID: (Human) 8945, (Rat) 361765

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Function(s)
ubiquitin-protein transferase activity protein binding beta-catenin binding ligase activity protein phosphorylated amino acid binding protein dimerization activity ubiquitin protein ligase activity
Process(es)
G2/M transition of mitotic cell cycle protein polyubiquitination stimulatory C-type lectin receptor signaling pathway protein dephosphorylation ubiquitin-dependent protein catabolic process signal transduction viral process Wnt signaling pathway protein ubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process protein destabilization mammary gland epithelial cell proliferation NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway regulation of circadian rhythm positive regulation of circadian rhythm regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of proteolysis negative regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated rhythmic process T cell receptor signaling pathway stress-activated MAPK cascade positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition branching involved in mammary gland duct morphogenesis regulation of canonical Wnt signaling pathway regulation of proteasomal protein catabolic process cellular response to organic cyclic compound protein catabolic process regulation of cell cycle
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