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Invitrogen
BTRC Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
BTRC Antibody (PA5-69160) in WB
Western blot was performed using Anti-BTRC Polyclonal Antibody (Product # PA5-69160) and a 69kDa band corresponding to BTRC was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of Neuro-2a (Lane 1), RSC96 (Lane 2), Rat Testis (Lane 3), Mouse Testis (Lane 4), Mouse Pancreas (Lane 5), PC-12 (Lane 6), Mouse Brain (Lane 7) and Rat Brain (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # I... View More
Product Details
PA5-69160
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
synthetic peptide directed towards the middle region of human BTRC (aa 551-600)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2689990
Product Specific Information
This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 86%
Target Information
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. This protein is homologous to Xenopus bTrCP1, yeast Met30, Neurospora Scon2 and Drosophila Slimb proteins. It interacts with HIV-1 Vpu and connects CD4 to the proteolytic machinery. It also associates specifically with phosphorylated IkappaBalpha and beta-catenin destruction motifs, probably functioning in multiple transcriptional programs by activating the NF-kappaB pathway and inhibiting the beta-catenin pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-transducin repeat-containing protein; Beta-TrCP; Beta-TrCP protein E3RS-IkappaB; btrc; E3RSIkappaB; F-box and WD repeats protein beta-TrCP; F-box/WD repeat-containing protein 1A; HOS; mE3RS-IkappaB; pIkappaB-E3 receptor subunit; SCF b-TRCP; Ubiquitin ligase FWD1
Gene Aliases: b-TrCP; beta-TrCP; Beta-Trcp1; Btrc; E3RS-IkappaB; E3RSIkappaB; Fbw1; Fbw1a; Fbxw1; FWD1; HOS; Kiaa4123; Slimb
UniProt ID: (Mouse) Q3ULA2
Entrez Gene ID: (Mouse) 12234
protein polyubiquitination
protein dephosphorylation
ubiquitin-dependent protein catabolic process
Wnt signaling pathway
protein ubiquitination
protein catabolic process
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
protein destabilization
mammary gland epithelial cell proliferation
regulation of circadian rhythm
positive regulation of circadian rhythm
regulation of I-kappaB kinase/NF-kappaB signaling
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of proteolysis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
rhythmic process
regulation of cell cycle
branching involved in mammary gland duct morphogenesis
regulation of proteasomal protein catabolic process
cellular response to organic cyclic compound
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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