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Gallus
Bacillus anthracis Lethal Factor A Polyclonal Antibody
Product Details
ABALFA
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Chicken anti-Bacillus anthracis Lethal Factor A
Conjugate
Form
Liquid
Concentration
0.5-2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS
Contains
0.075% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
This antibody is suitable for ELISA and Western Blotting procedures. By Western blot, a 1:10,000 dilution of the IgY was an effective probe for 16 ng of Lethal Factor A. By Direct ELISA, the half-point titre was 1:38,000 dilution using a 2 µg/mL coating of Lethal Factor A.
Target Information
Anthrax toxin is a three-protein exotoxin secreted by virulent strains of the bacterium Bacillus anthracis, the causative agent of anthrax. Anthrax toxin is composed of a cell-binding protein, known as protective antigen (PA), and two enzyme components, called edema factor (EF) and lethal factor (LF). These three protein components act together to impart their physiological effects. Assembled complexes containing the toxin components are endocytosed. In the endosome, the enzymatic components of the toxin translocate into the cytoplasm of a target cell. Once in the cytosol, the enzymatic components of the toxin disrupts various immune cell functions, namely cellular signaling and cell migration. The toxin may even induce cell lysis, as is observed for macrophage cells. Anthrax toxin allows the bacteria to evade the immune system, proliferate, and ultimately kill the host animal. Research on anthrax toxin also provides insight into the generation of macromolecular assemblies, and on protein translocation, pore formation, endocytosis, and other biochemical processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Anthrax LF; B. anthracis LF
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