Invitrogen

Bcl-2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

22 References

View all (186) BCL-2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Bcl-2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 24

Bcl-2 Antibody (PA5-27094) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Bcl-2 was performed in THP-1 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Bcl-2 Polyclonal Antibody (Product # PA5-27094) diluted at 1:500. Blue: Fluoroshield with DAPI.

Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Bcl-2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Bcl-2 Antibody — Figure 3. Combined treatment downregulates the expression of anti-apoptotic proteins and enhances the expression of pro-apoptotic proteins. Western blot analysis results of (A) anti-apoptotic and (B) pro-apoptotic protein expression 24 h following cis, bor and cis plus bortezomib combination treatment. C, control; cis, cisplatin; bor, bortezomib; Bcl-2, B-cell lymphoma-2; Bcl-xL, B-cell lyphoma-extra large; Bim, B-cell lyphoma-2-like 11; Bik, Bcl-2-interacting killer. {TM}

Product Details

PA5-27094

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 1 and 48 of Human BCL2

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2544570

Product Specific Information

Recommended positive controls: THP-1, HL-60, mouse spleen, PA-1, OKT3.

Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Cat (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Panax ginseng ameliorates hepatorenal oxidative alterations induced by commercially used cypermethrin in male rats: experimental and molecular docking approaches.

Environmental science and pollution research international

Elblehi SS,Hafez MH,El-Far AH

PA5-27094 was used in Immunohistochemistry to understand its protective properties against CYP-induced toxicity.

Sun Oct 01 00:00:00 EDT 2023

Curative Effect of AD-MSCs against Cisplatin-Induced Hepatotoxicity in Rats is Potentiated by Azilsartan: Targeting Oxidative Stress, MAPK, and Apoptosis Signaling Pathways.

Stem cells international

Bekhit AA,Beshay ON,Fawzy MA,Abdel-Hafez SMN,Batiha GE,Ataya FS,Fathy M

PA5-27094 was used in Western Blot to demonstrate that AZIL improves the hepatoprotective potential of AD-MSCs against CISP-induced hepatotoxicity by modulating oxidative stress, mitogen-activated protein kinase, and apoptotic pathways.

Thu Nov 02 00:00:00 EDT 2023

Enhancing the anticancer potential of metformin: fabrication of efficient nanospanlastics, in vitro cytotoxic studies on HEP-2 cells and reactome enhanced pathway analysis.

International journal of pharmaceutics: X

Raafat SN,El Wahed SA,Badawi NM,Saber MM,Abdollah MRA

PA5-27094 was used in Western Blot to highlight, using PEA, that MET can modulate multiple pathways implicated in carcinogenesis.

Fri Dec 15 00:00:00 EST 2023

Memantine/Aripiprazole Combination Alleviates Cognitive Dysfunction in Valproic Acid Rat Model of Autism: Hippocampal CREB/BDNF Signaling and Glutamate Homeostasis.

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics

Zohny SM,Habib MZ,Mohamad MI,Elayat WM,Elhossiny RM,El-Salam MFA,Hassan GAM,Aboul-Fotouh S

PA5-27094 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to draw attention to the favorable cognitive effects of memantine/aripiprazole combination in autistic subjects which could be mediated via enhancing CREB/BDNF signaling with increased expression of astrocytic Glt-1 and restoration of GABA/glutamate balance, leading to inhibition of hippocampal NFTs formation and neuronal apoptosis.

Wed Mar 01 00:00:00 EST 2023

Preventive Effect of Limosilactobacillus fermentum SCHY34 on Lead Acetate-Induced Neurological Damage in SD Rats.

Frontiers in nutrition

Long X,Wu H,Zhou Y,Wan Y,Kan X,Gong J,Zhao X

PA5-27094 was used in Western Blotting to show that L. fermentum SCHY34 showed a great biological scavenging and potential effect on alleviating the toxicity of lead ions.

Sat Jul 16 00:00:00 EDT 2022

Orientin, a Bio-Flavonoid from Trigonella hamosa L., Regulates COX-2/PGE-2 in A549 Cell Lines via miR-26b and miR-146a.

Pharmaceuticals (Basel, Switzerland)

Khalil HE,Ibrahim HM,Ahmed EA,Emeka PM,Alhaider IA

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Western Blot

Thu Jan 27 00:00:00 EST 2022

Orientin, a Bio-Flavonoid from Trigonella hamosa L., Regulates COX-2/PGE-2 in A549 Cell Lines via miR-26b and miR-146a.

Pharmaceuticals (Basel, Switzerland)

Khalil HE,Ibrahim HM,Ahmed EA,Emeka PM,Alhaider IA

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Western Blot

Thu Jan 27 00:00:00 EST 2022

Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway.

Translational andrology and urology

Su Y,Xu J,Chen S,Feng J,Li J,Lei Z,Qiao L,Wang Y,Zeng D

PA5-27094 was used in Western Blotting to investigate the protective effects of Astragaloside IV (AS-IV) on ischemia-reperfusion (I/R) renal injury based on the keap1-Nrf2/ARE signaling pathway.

Mon Aug 01 00:00:00 EDT 2022

Astragaloside IV protects against ischemia/reperfusion (I/R)-induced kidney injury based on the Keap1-Nrf2/ARE signaling pathway.

Translational andrology and urology

Su Y,Xu J,Chen S,Feng J,Li J,Lei Z,Qiao L,Wang Y,Zeng D

PA5-27094 was used in Western Blotting to investigate the protective effects of Astragaloside IV (AS-IV) on ischemia-reperfusion (I/R) renal injury based on the keap1-Nrf2/ARE signaling pathway.

Mon Aug 01 00:00:00 EDT 2022

A Nano-Liposomal Formulation of Caffeic Acid Phenethyl Ester Modulates Nrf2 and NF-κβ Signaling and Alleviates Experimentally Induced Acute Pancreatitis in a Rat Model.

Antioxidants (Basel, Switzerland)

Shahin NN,Shamma RN,Ahmed IS

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Immunohistochemistry

Sun Aug 07 00:00:00 EDT 2022

A Nano-Liposomal Formulation of Caffeic Acid Phenethyl Ester Modulates Nrf2 and NF-κβ Signaling and Alleviates Experimentally Induced Acute Pancreatitis in a Rat Model.

Antioxidants (Basel, Switzerland)

Shahin NN,Shamma RN,Ahmed IS

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Immunohistochemistry

Sun Aug 07 00:00:00 EDT 2022

Infarct-preconditioning exosomes of umbilical cord mesenchymal stem cells promoted vascular remodeling and neurological recovery after stroke in rats.

Stem cell research & therapy

Ye YC,Chang ZH,Wang P,Wang YW,Liang J,Chen C,Wang JJ,Sun HT,Wang Y,Li XH

PA5-27094 was used in Western Blotting to investigate if exosomes secreted in response to infarction microenvironment could have further therapeutic effects.

Thu Jul 28 00:00:00 EDT 2022

Necrostatin-1 mitigates renal ischaemia-reperfusion injury - time dependent - via aborting the interacting protein kinase (RIPK-1)-induced inflammatory immune response.

Clinical and experimental pharmacology & physiology

Ashour H,Hashem HA,Khowailed AA,Rashed LA,Hassan RM,Soliman AS

PA5-27094 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that RIPK1 inhibition ameliorates the inflammatory immune response induced by renal IRI.

Fri Apr 01 00:00:00 EDT 2022

Possible Implication of Nrf2, PPAR-γ and MAPKs Signaling in the Protective Role of Mangiferin against Renal Ischemia/Reperfusion in Rats.

Pharmaceuticals (Basel, Switzerland)

Gendy AM,El-Gazar AA,Ragab GM,Al-Mokaddem AK,El-Haddad AE,Selim HMRM,Yousef EM,Hamed NO,Ibrahim SSA

PA5-27094 was used in Western Blotting to track further non-investigated modulatory signals of Mang against the renal Is/R model involving nuclear factor erythroid 2-related factor (Nrf)2/heme oxygenase (HO)-1, peroxisome proliferator-activated receptor (PPAR)-γ/nuclear factor (NF)-κB, p38 mitogen-activated protein kinase (MAPK), and c-Jun N-terminal kinase (JNK) signaling.

Wed Dec 21 00:00:00 EST 2022

Date Palm Pollen Extract Avert Doxorubicin-Induced Cardiomyopathy Fibrosis and Associated Oxidative/Nitrosative Stress, Inflammatory Cascade, and Apoptosis-Targeting Bax/Bcl-2 and Caspase-3 Signaling Pathways.

Animals : an open access journal from MDPI

Elblehi SS,El-Sayed YS,Soliman MM,Shukry M

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Immunohistochemistry

Sat Mar 20 00:00:00 EDT 2021

Animals : an open access journal from MDPI

Elblehi SS,El-Sayed YS,Soliman MM,Shukry M

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Immunohistochemistry

Sat Mar 20 00:00:00 EDT 2021

Effects of lncRNA ANRIL-knockdown on the proliferation, apoptosis and cell cycle of gastric cancer cells.

Oncology letters

Hu X,Lou T,Yuan C,Wang Y,Tu X,Wang Y,Zhang T

PA5-27094 was used in Western Blotting to construct drug-resistant gastric cancer cell lines using the stepwise continuous selection method.

Sun Aug 01 00:00:00 EDT 2021

Cardiac Protective Effect of Kirenol against Doxorubicin-Induced Cardiac Hypertrophy in H9c2 Cells through Nrf2 Signaling via PI3K/AKT Pathways.

International journal of molecular sciences

Alzahrani AM,Rajendran P,Veeraraghavan VP,Hanieh H

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Western Blot

Tue Mar 23 00:00:00 EDT 2021

Human umbilical cord-derived mesenchymal stem cells and human cord blood mononuclear cells protect against cisplatin-induced acute kidney injury in rat models.

Experimental and therapeutic medicine

Xu Q,Yan P,Duan XJ,Wu X,Chen XJ,Luo M,Peng JC,Feng LX,Liu J,Zhong HL,Cheng W,Zou QY,Duan SB

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Western Blot

Tue Dec 01 00:00:00 EST 2020

Human urine-derived stem cells protect against renal ischemia/reperfusion injury in a rat model via exosomal miR-146a-5p which targets IRAK1.

Theranostics

Li X,Liao J,Su X,Li W,Bi Z,Wang J,Su Q,Huang H,Wei Y,Gao Y,Li J,Liu L,Wang C

PA5-27094 was used in Western Blotting to study the protective effects of human urine-derived stem cells against a rat model of renal ischemia/reperfusion injury.

Tue Jun 01 00:00:00 EDT 2021

An iPSC-Derived Neuron Model of CLN3 Disease Facilitates Small Molecule Phenotypic Screening.

ACS pharmacology & translational science

Kinarivala N,Morsy A,Patel R,Carmona AV,Sajib MS,Raut S,Mikelis CM,Al-Ahmad A,Trippier PC

PA5-27094 was used in Western Blot to report the characterization of the first CLN3 patient-specific induced pluripotent stem cell (iPSC)-derived model of the blood-brain barrier and establish the suitability of an iPSC-derived neuron model of the disease to facilitate compound screening.

Fri Oct 09 00:00:00 EDT 2020

Kaempferol Inhibits Zearalenone-Induced Oxidative Stress and Apoptosis via the PI3K/Akt-Mediated Nrf2 Signaling Pathway: In Vitro and In Vivo Studies.

International journal of molecular sciences

Rajendran P,Ammar RB,Al-Saeedi FJ,Mohamed ME,ElNaggar MA,Al-Ramadan SY,Bekhet GM,Soliman AM

PA5-27094 was used in Western Blotting to demonstrate that kaempferol possesses notable hepatoprotective roles against zearalenone-induced damage, potentially through the stimulation of Nrf2/HO-1 cascades and PI3K/Akt signalling.

Mon Dec 28 00:00:00 EST 2020

Molecular mechanisms underlying histological and biochemical changes induced by nitrate in rat liver and the efficacy of S-Allylcysteine.

Ultrastructural pathology

Kattaia AA,Abd El-Baset SA,Mohamed EM,Abdul-Maksou RS,Elfakharany YM

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Immunohistochemistry

Tue Jun 06 00:00:00 EDT 2017

Molecular mechanisms underlying histological and biochemical changes induced by nitrate in rat liver and the efficacy of S-Allylcysteine.

Ultrastructural pathology

Kattaia AA,Abd El-Baset SA,Mohamed EM,Abdul-Maksou RS,Elfakharany YM

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Immunohistochemistry

Tue Jun 06 00:00:00 EDT 2017

Synergistic effects of cisplatin and proteasome inhibitor bortezomib on human bladder cancer cells.

Oncology letters

Konac E,Varol N,Kiliccioglu I,Bilen CY

Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Western Blot

Wed Jul 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: Apoptosis regulator Bcl-2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; Bcl2-like protein; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

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Gene Aliases: AW986256; Bcl-2; BCL2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PPP1R50

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UniProt ID: (Human) P10415, (Rat) P49950, (Mouse) P10417

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Entrez Gene ID: (Human) 596, (Rat) 24224, (Mouse) 12043

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Function(s)

protease binding protein binding transcription factor binding channel activity channel inhibitor activity ubiquitin protein ligase binding identical protein binding protein homodimerization activity sequence-specific DNA binding protein heterodimerization activity BH3 domain binding protein phosphatase 2A binding protein phosphatase binding BH domain binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Bcl-2 Polyclonal Antibody

Bcl-2 Antibody (PA5-27094) in ICC/IF

Product Details

PA5-27094

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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