Invitrogen

Bcl-2 Monoclonal Antibody (Bcl-2-100)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

27 Published Figures

62 References

View all (186) BCL-2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 36

Bcl-2 Antibody (13-8800) in IHC (P)

Immunohistochemical analysis of Bcl-2 was performed using formalin-fixed paraffin-embedded human colon cancer (Diffuse large B cell lymphoma) tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at ... View More

Bcl-2 Antibody in Immunocytochemistry (ICC/IF)

Bcl-2 Antibody in Immunohistochemistry (IHC)

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Bcl-2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues tested owing to their inherent genetic constitution. Relative expression of Bcl-2 was observed in HL-60, U-937, Raji, HeLa which are reported to be positive and not in HT-29 and A-375. In addition, expression of Bcl-2 was higher in mouse spleen in comparison to mouse skeletal muscle and mouse brain using Anti-Bcl-2 Monoclonal Antibody (Bcl-2-100) (Product # 13-8800) in western blot. {RE}

Product Details

13-8800

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 29 publications 29 publications

Immunohistochemistry (IHC)

Assay-dependent

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

View 10 publications 10 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

Assay-dependent

View 2 publications 2 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Bacteria, Chicken, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

Bcl-2-100

Immunogen

Synthetic peptide corresponding to residues 41-54 of the human Bcl-2 protein. This sequence is not shared by the mouse Bcl-2 protein.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533042

Target Information

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LINC01311 exerts an inhibitory effect in thyroid cancer progression by targeting the miR-146b-5p/IMPA2 axis.

Translational oncology

Liu M,Zhang L,Hu J,Yan C,Zhang Y,Yan Z

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Sun Jan 01 00:00:00 EST 2023

Hsa_circ_0129047 sponges miR-665 to attenuate lung adenocarcinoma progression by upregulating protein tyrosine phosphatase receptor type B.

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology

Xia X,Fan J,Fan Z

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Wed Mar 01 00:00:00 EST 2023

Rubus Capped Zinc Oxide Nanoparticles Induce Apoptosis in MCF-7 Breast Cancer Cells.

Molecules (Basel, Switzerland)

George BP,Rajendran NK,Houreld NN,Abrahamse H

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Immunocytochemistry

Thu Oct 13 00:00:00 EDT 2022

Rubus Capped Zinc Oxide Nanoparticles Induce Apoptosis in MCF-7 Breast Cancer Cells.

Molecules (Basel, Switzerland)

George BP,Rajendran NK,Houreld NN,Abrahamse H

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Immunocytochemistry

Thu Oct 13 00:00:00 EDT 2022

miR‑205‑3p promotes lung cancer progression by targeting APBB2.

Molecular medicine reports

Xu LB,Xiong J,Zhang YH,Dai Y,Ren XP,Ren YJ,Han D,Wei SH,Qi M

13-8800 was used in Western Blot to investigate the potential promotive impact of miR‚205‚3p on non‚small cell lung cancer processes and if theoretical evidence for miR‚205‚3p as a potential clinical gene therapy target exists.

Sun Aug 01 00:00:00 EDT 2021

miR‑205‑3p promotes lung cancer progression by targeting APBB2.

Molecular medicine reports

Xu LB,Xiong J,Zhang YH,Dai Y,Ren XP,Ren YJ,Han D,Wei SH,Qi M

Sun Aug 01 00:00:00 EDT 2021

miR‑125a‑5p reverses epithelial‑mesenchymal transition and restores drug sensitivity by negatively regulating TAFAZZIN signaling in breast cancer.

Molecular medicine reports

Li D,Chen L,Zhang X,Wang Y,Huang C,Li J,He F,He W

13-8800 was used in Western Blotting to demonstrate that miR‚125a‚5p inhibited the EMT of MCF‚7/adriamycin (Adr) breast cancer cells, as well as decreased the proliferation and increased the apoptosis of breast cancer cells treated with Adr/docetaxel.

Mon Nov 01 00:00:00 EDT 2021

miR‑125a‑5p reverses epithelial‑mesenchymal transition and restores drug sensitivity by negatively regulating TAFAZZIN signaling in breast cancer.

Molecular medicine reports

Li D,Chen L,Zhang X,Wang Y,Huang C,Li J,He F,He W

Mon Nov 01 00:00:00 EDT 2021

Vitamin E TPGS 1000 Induces Apoptosis in the K562 Cell Line: Implications for Chronic Myeloid Leukemia.

Oxidative medicine and cellular longevity

Calvo-Alvarez J,Jimenez-Del-Rio M,Velez-Pardo C

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Tue Dec 14 00:00:00 EST 2021

HDAC inhibitors promote pancreatic stellate cell apoptosis and relieve pancreatic fibrosis by upregulating miR-15/16 in chronic pancreatitis.

Human cell

Ji T,Feng W,Zhang X,Zang K,Zhu X,Shang F

13-8800 was used in Western Blotting to find that miR-15 and miR-16 decreased significantly in chronic pancreatitis and HDAC inhibition could recover the levels of these two miRNAs.

Thu Oct 01 00:00:00 EDT 2020

HDAC inhibitors promote pancreatic stellate cell apoptosis and relieve pancreatic fibrosis by upregulating miR-15/16 in chronic pancreatitis.

Human cell

Ji T,Feng W,Zhang X,Zang K,Zhu X,Shang F

13-8800 was used in Western Blotting to find that miR-15 and miR-16 decreased significantly in chronic pancreatitis and HDAC inhibition could recover the levels of these two miRNAs.

Thu Oct 01 00:00:00 EDT 2020

MiR-181c-5p Mitigates Tumorigenesis in Cervical Squamous Cell Carcinoma via Targeting Glycogen Synthase Kinase 3β Interaction Protein (GSKIP).

OncoTargets and therapy

Li N,Cheng C,Wang T

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Fri Apr 15 00:00:00 EDT 2022

Silencing of long‑chain non‑coding RNA GAS5 in osteoarthritic chondrocytes is mediated by targeting the miR‑34a/Bcl‑2 axis.

Molecular medicine reports

Ji Q,Qiao X,Liu Y,Wang D,Yan J

13-8800 was used in Western Blot to indicate that GAS5 participated in the development of OA by regulating the biological behavior of chondrocytes via the miR‚34a/Bcl‚2 axis.

Sun Mar 01 00:00:00 EST 2020

Neuroprotective Effects of Growth Hormone (GH) and Insulin-Like Growth Factor Type 1 (IGF-1) after Hypoxic-Ischemic Injury in Chicken Cerebellar Cell Cultures.

International journal of molecular sciences

Baltazar-Lara R,Ávila-Mendoza J,Martínez-Moreno CG,Carranza M,Pech-Pool S,Vázquez-Martínez O,Díaz-Muñoz M,Luna M,Arámburo C

13-8800 was used in Western Blot to show that GH, either exogenously administered and/or locally expressed, can act as a neuroprotective factor in response to hypoxic-ischemic injury, and that this effect may be mediated, at least partially, through IGF-1 expression.

Tue Dec 29 00:00:00 EST 2020

Neuroprotective Effects of Growth Hormone (GH) and Insulin-Like Growth Factor Type 1 (IGF-1) after Hypoxic-Ischemic Injury in Chicken Cerebellar Cell Cultures.

International journal of molecular sciences

Baltazar-Lara R,Ávila-Mendoza J,Martínez-Moreno CG,Carranza M,Pech-Pool S,Vázquez-Martínez O,Díaz-Muñoz M,Luna M,Arámburo C

Tue Dec 29 00:00:00 EST 2020

An Anti-PSMA Immunotoxin Reduces Mcl-1 and Bcl2A1 and Specifically Induces in Combination with the BAD-Like BH3 Mimetic ABT-737 Apoptosis in Prostate Cancer Cells.

Cancers

Masilamani AP,Dettmer-Monaco V,Monaco G,Cathomen T,Kuckuck I,Schultze-Seemann S,Huber N,Wolf P

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Mon Jun 22 00:00:00 EDT 2020

An Anti-PSMA Immunotoxin Reduces Mcl-1 and Bcl2A1 and Specifically Induces in Combination with the BAD-Like BH3 Mimetic ABT-737 Apoptosis in Prostate Cancer Cells.

Cancers

Masilamani AP,Dettmer-Monaco V,Monaco G,Cathomen T,Kuckuck I,Schultze-Seemann S,Huber N,Wolf P

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Mon Jun 22 00:00:00 EDT 2020

The mTORC1/S6K/PDCD4/eIF4A Axis Determines Outcome of Mitotic Arrest.

Cell reports

Moustafa-Kamal M,Kucharski TJ,El-Assaad W,Abbas YM,Gandin V,Nagar B,Pelletier J,Topisirovic I,Teodoro JG

13-8800 was used in Western Blot to indicate that the mTORC1/S6K/PDCD4/eIF4A axis has a pivotal role in the death versus slippage decision during mitotic arrest and may be exploited clinically to treat tumors resistant to anti-mitotic agents.

Tue Oct 06 00:00:00 EDT 2020

miRNA‑183‑5p.1 promotes the migration and invasion of gastric cancer AGS cells by targeting TPM1.

Oncology reports

Lin J,Shen J,Yue H,Cao Z

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Sun Dec 01 00:00:00 EST 2019

BH3 profiling discriminates the anti‑apoptotic status of 5‑fluorouracil‑resistant colon cancer cells.

Oncology reports

Ishikawa K,Kawano Y,Arihara Y,Kubo T,Takada K,Murase K,Miyanishi K,Kobune M,Kato J

13-8800 was used in Western Blotting to indicate that BH3 profiling facilitates the identification of the functional role of anti-apoptotic proteins during drug resistance and has clinical implications for colon cancer in targeting specific proteins such as BCLXL.

Sun Dec 01 00:00:00 EST 2019

The transcription factor ZEB1 promotes chemoresistance in prostate cancer cell lines.

Asian journal of andrology

Orellana-Serradell O,Herrera D,Castellón EA,Contreras HR

13-8800 was used in Western Blot to show that modulation of MRP4 could be a mediator of ZEB1-related resistance to docetaxel in prostate cancer, making it a possible marker for chemotherapy response in patients who do not express MDR1.

Mon Aug 17 00:00:00 EDT 2020

Regenerative Effect of Growth Hormone (GH) in the Retina after Kainic Acid Excitotoxic Damage.

International journal of molecular sciences

Martinez-Moreno CG,Epardo D,Balderas-Márquez JE,Fleming T,Carranza M,Luna M,Harvey S,Arámburo C

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Tue Sep 10 00:00:00 EDT 2019

Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.

eLife

Kang Y,Anderson AJ,Jackson TD,Palmer CS,De Souza DP,Fujihara KM,Stait T,Frazier AE,Clemons NJ,Tull D,Thorburn DR,McConville MJ,Ryan MT,Stroud DA,Stojanovski D

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Mon Nov 04 00:00:00 EST 2019

Pluronic micelles encapsulated curcumin manifests apoptotic cell death and inhibits pro-inflammatory cytokines in human breast adenocarcinoma cells.

Cancer reports (Hoboken, N.J.)

Vaidya FU,Sharma R,Shaikh S,Ray D,Aswal VK,Pathak C

13-8800 was used in ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot to demonstrate that Pluronic micelles encapsulated curcumin is stable, nontoxic to normal cells, and can effectively inhibit cell proliferation and release pro-inflammatory cytokines against cancer cells.

Fri Feb 01 00:00:00 EST 2019

Pluronic micelles encapsulated curcumin manifests apoptotic cell death and inhibits pro-inflammatory cytokines in human breast adenocarcinoma cells.

Cancer reports (Hoboken, N.J.)

Vaidya FU,Sharma R,Shaikh S,Ray D,Aswal VK,Pathak C

Fri Feb 01 00:00:00 EST 2019

Emodin inhibits colon cancer by altering BCL-2 family proteins and cell survival pathways.

Cancer cell international

Saunders IT,Mir H,Kapur N,Singh S

13-8800 was used in Western Blotting to investigate the effect of a natural agent, Emodin, on CoCa.

Fri Apr 08 00:00:00 EDT 2022

Induction of Lysosome-associated Protein Transmembrane 4 Beta via Sulfatase 2 Enhances Autophagic Flux in Liver Cancer Cells.

Hepatology communications

Ha Y,Fang Y,Romecin Duran PA,Tolosa EJ,Moser CD,Fernandez-Zapico ME,Roberts LR

13-8800 was used in Western Blotting to demonstrate a role for SULF2 in the regulation of autophagic flux that is mediated through LAPTM4B induction in hepatocellular carcinoma (HCC) cells, and provide a foundation for future translational efforts targeting autophagy in liver malignancies.

Fri Nov 01 00:00:00 EDT 2019

CXCR4 Promotes Neuroblastoma Growth and Therapeutic Resistance through miR-15a/16-1-Mediated ERK and BCL2/Cyclin D1 Pathways.

Cancer research

Klein S,Abraham M,Bulvik B,Dery E,Weiss ID,Barashi N,Abramovitch R,Wald H,Harel Y,Olam D,Weiss L,Beider K,Eizenberg O,Wald O,Galun E,Pereg Y,Peled A

13-8800 was used in Flow cytometry/Cell sorting to provide a mechanistic rationale for combination therapy of CXCR4 and BCL-2 inhibitors to treat a common and commonly aggressive pediatric cancer.

Thu Mar 15 00:00:00 EDT 2018

Bcl-2 Overexpression Improves Survival and Efficacy of Neural Stem Cell-Mediated Enzyme Prodrug Therapy.

Stem cells international

Mooney R,Majid AA,Mota D,He A,Aramburo S,Flores L,Covello-Batalla J,Machado D,Gonzaga J,Aboody KS

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Flow Cytometry

Sun Aug 20 00:00:00 EDT 2023

Effects of Lycium barbarum polysaccharides on the damage to human endometrial stromal cells induced by hydrogen peroxide.

Molecular medicine reports

Shan T,Shan T,Liu F,Zheng H,Li G

13-8800 was used in Western Blotting to study the effects of Lycium barbarum polysaccharides on the damage to human endometrial stromal cells induced by hydrogen peroxide.

Wed Feb 01 00:00:00 EST 2017

Expression of cFLIPL Determines the Basal Interaction of Bcl-2 With Beclin-1 and Regulates p53 Dependent Ubiquitination of Beclin-1 During Autophagic Stress.

Journal of cellular biochemistry

Ranjan K,Pathak C

13-8800 was used in western blot to study regulation of p53 dependent ubiquitination of Beclin-1 during autophagic stress and basal interaction of Bcl-2 with Beclin-1 by expression of cFLIPL

Mon Aug 01 00:00:00 EDT 2016

Expression of FADD and cFLIP_L balances mitochondrial integrity and redox signaling to substantiate apoptotic cell death.

Molecular and cellular biochemistry

Ranjan K,Pathak C

138800 was used in western blot to elucidate how FADD contributes to mitochondrial-associated apoptosis

Tue Nov 01 00:00:00 EDT 2016

FADD regulates NF-κB activation and promotes ubiquitination of cFLIPL to induce apoptosis.

Scientific reports

Ranjan K,Pathak C

13-8800 was used in western blot to show how FADD regulates NF-kappaB activation and promotes ubiquitination of cFLIPL, which induces apoptosis

Mon Mar 14 00:00:00 EDT 2016

Direct antiapoptotic effects of growth hormone are mediated by PI3K/Akt pathway in the chicken bursa of Fabricius.

General and comparative endocrinology

Luna-Acosta JL,Alba-Betancourt C,Martínez-Moreno CG,Ramírez C,Carranza M,Luna M,Arámburo C

13-8800 was used in western blot to investigate the role of growth hormone in the Bursa

Tue Dec 01 00:00:00 EST 2015

Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature.

Diagnostic pathology

Ertoy Baydar D,Kosemehmetoglu K,Aydin O,Bridge JA,Buyukeren B,Aki FT

13-8800 was used in immunohistochemistry to present two cases of primary sclerosing epithelioid fibrosarcoma of the kidney

Fri Oct 09 00:00:00 EDT 2015

Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature.

Diagnostic pathology

Ertoy Baydar D,Kosemehmetoglu K,Aydin O,Bridge JA,Buyukeren B,Aki FT

13-8800 was used in immunohistochemistry to present two cases of primary sclerosing epithelioid fibrosarcoma of the kidney

Fri Oct 09 00:00:00 EDT 2015

Automated quantitative analysis of tissue microarray of 443 patients with colorectal adenocarcinoma: low expression of Bcl-2 predicts poor survival.

Journal of the American College of Surgeons

Nicholson AD,Guo X,Sullivan CA,Cha CH

13-8800 was used in immunohistochemistry - paraffin section to investigate Bcl2 expression in colorectal adenocarcinoma

Sat Nov 01 00:00:00 EDT 2014

Cytoprotective mechanisms in rats lung parenchyma with zinc deprivation.

Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine

Biaggio VS,Alvarez-Olmedo DG,Perez Chaca MV,Salvetti NR,Valdez SR,Fanelli MA,Ortega HH,Gomez NN,Gimenez MS

13-8800 was used in immunohistochemistry to study the effect of zinc deficiency on airway epithelium

Tue Apr 01 00:00:00 EDT 2014

U1 Adaptor Oligonucleotides Targeting BCL2 and GRM1 Suppress Growth of Human Melanoma Xenografts In Vivo.

Molecular therapy. Nucleic acids

Goraczniak R,Wall BA,Behlke MA,Lennox KA,Ho ES,Zaphiros NH,Jakubowski C,Patel NR,Zhao S,Magaway C,Subbie SA,Jenny Yu L,Lacava S,Reuhl KR,Chen S,Gunderson SI

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Tue May 14 00:00:00 EDT 2013

U1 Adaptor Oligonucleotides Targeting BCL2 and GRM1 Suppress Growth of Human Melanoma Xenografts In Vivo.

Molecular therapy. Nucleic acids

Goraczniak R,Wall BA,Behlke MA,Lennox KA,Ho ES,Zaphiros NH,Jakubowski C,Patel NR,Zhao S,Magaway C,Subbie SA,Jenny Yu L,Lacava S,Reuhl KR,Chen S,Gunderson SI

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Western Blot

Tue May 14 00:00:00 EDT 2013

Enhanced heterodimerization of Bax by Bcl-2 mutants improves irradiated cell survival.

Apoptosis : an international journal on programmed cell death

Verma YK,Raghav PK,Raj HG,Tripathi RP,Gangenahalli GU

13-8800 was used in immunoprecipitation to compared the effects and mechanism of Bcl-2 and its functional variants on irradiated cell survival.

Fri Feb 01 00:00:00 EST 2013

Expression of peroxisome proliferator activated receptor-gamma (PPAR-γ) in human non-small cell lung carcinoma: correlation with clinicopathological parameters, proliferation and apoptosis related molecules and patients' survival.

Pathology oncology research : POR

Giaginis C,Politi E,Alexandrou P,Sfiniadakis J,Kouraklis G,Theocharis S

13-8800 was used in immunohistochemistry (paraffin) to determine the clinical significance of PPAR-γ expression in non-small cell lung carcinoma.

Mon Oct 01 00:00:00 EDT 2012

The relation of pericoronal third molar follicle dimension and bcl-2/ki-67 expression: An immunohistochemical study.

Dental research journal

Razavi SM,Hasheminia D,Mehdizade M,Movahedian B,Keshani F

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Immunohistochemistry

Sat Dec 01 00:00:00 EST 2012

The relation of pericoronal third molar follicle dimension and bcl-2/ki-67 expression: An immunohistochemical study.

Dental research journal

Razavi SM,Hasheminia D,Mehdizade M,Movahedian B,Keshani F

Published figure using Bcl-2 monoclonal antibody (Product # 13-8800) in Immunohistochemistry

Sat Dec 01 00:00:00 EST 2012

Oleanolic acid initiates apoptosis in non-small cell lung cancer cell lines and reduces metastasis of a B16F10 melanoma model in vivo.

PloS one

Lúcio KA,Rocha Gda G,Monção-Ribeiro LC,Fernandes J,Takiya CM,Gattass CR

13-8800 was used in western blot to test if oleanolic acid circumvents the mechanisms of drug resistance in non-small cell lung cancer

Mon Apr 23 00:00:00 EDT 2012

Oleanolic acid initiates apoptosis in non-small cell lung cancer cell lines and reduces metastasis of a B16F10 melanoma model in vivo.

PloS one

Lúcio KA,Rocha Gda G,Monção-Ribeiro LC,Fernandes J,Takiya CM,Gattass CR

13-8800 was used in western blot to test if oleanolic acid circumvents the mechanisms of drug resistance in non-small cell lung cancer

Mon Apr 23 00:00:00 EDT 2012

Paracrine factors secreted by endothelial progenitor cells prevent oxidative stress-induced apoptosis of mature endothelial cells.

Atherosclerosis

Yang Z,von Ballmoos MW,Faessler D,Voelzmann J,Ortmann J,Diehm N,Kalka-Moll W,Baumgartner I,Di Santo S,Kalka C

Thu Jul 01 00:00:00 EDT 2010

Idiopathic cervical fibrosis--a new member of IgG4-related sclerosing diseases: report of 4 cases, 1 complicated by composite lymphoma.

The American journal of surgical pathology

Cheuk W,Tam FK,Chan AN,Luk IS,Yuen AP,Chan WK,Hung TC,Chan JK

13-8800 was used in immunohistochemistry - paraffin section to report on four cases of idiopathic cervical fibrosis

Mon Nov 01 00:00:00 EDT 2010

The cytological, clinical, and pathological features of the cribriform-morular variant of papillary thyroid carcinoma and mutation analysis of CTNNB1 and BRAF genes.

Thyroid : official journal of the American Thyroid Association

Jung CK,Choi YJ,Lee KY,Bae JS,Kim HJ,Yoon SK,Son YI,Chung JH,Oh YL

13-8800 was used in immunohistochemistry - paraffin section to characterize cribriform-morular variant of papillary thyroid carcinoma

Sat Aug 01 00:00:00 EDT 2009

Inhibition of receptor-mediated apoptosis upon Bcl-2 overexpression is not associated with increased antioxidant status.

Biochemical and biophysical research communications

Thomson SJ,Cox AG,Cuddihy SL,Pullar JM,Hampton MB

13-8800 was used in western blot to challenge the hypothesis that the anti-apoptotic properties of Bcl-2 are linked to a global increase in antioxidant status

Fri Oct 10 00:00:00 EDT 2008

Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis.

Journal of Korean medical science

Yoo J,Jung JH,Lee MA,Seo KJ,Shim BY,Kim SH,Cho DG,Ahn MI,Kim CH,Cho KD,Kang SJ,Kim HK

13-8800 was used in immunohistochemistry - paraffin section to study the immunohistochemistry of non-small cell lung cancer

Sun Apr 01 00:00:00 EDT 2007

Localised breast cancers may have systemic influences on skin and hair.

Journal of clinical pathology

Lawson JS,Tran DD

13-8800 was used in immunohistochemistry - paraffin section to correlate biomarkers in breast cancer and breast skin in the same patients

Thu Feb 01 00:00:00 EST 2007

Regression of gastric large B-Cell lymphoma accompanied by a florid lymphoma-like T-cell reaction: immunomodulatory effect of Ganoderma lucidum (Lingzhi)?

International journal of surgical pathology

Cheuk W,Chan JK,Nuovo G,Chan MK,Fok M

13-8800 was used in immunohistochemistry - paraffin section to present a case of complete regression of high-grade lymphoma

Sun Apr 01 00:00:00 EDT 2007

Cell-penetrating anti-native DNA antibodies trigger apoptosis through both the neglect and programmed pathways.

Journal of autoimmunity

Rivadeneyra-Espinoza L,Ruiz-Argüelles A

13-8800 was used in immunocytochemistry to investigate how antibodies to double stranded-DNA induce apoptosis

Wed Feb 01 00:00:00 EST 2006

Differential expression of apoptosis-related proteins in various cellular components of ameloblastomas.

International journal of oral and maxillofacial surgery

Luo HY,Yu SF,Li TJ

13-8800 was used in immunohistochemistry - paraffin section to use immunohistochemistry to study apoptosis-related proteins in cases of ameloblastoma

Tue Aug 01 00:00:00 EDT 2006

Extent, relationship and prognostic significance of apoptosis and cell proliferation in synovial sarcoma.

European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)

Sun B,Sun Y,Wang J,Zhao X,Wang X,Hao X

13-8800 was used in immunohistochemistry - paraffin section to examine apoptosis and cell proliferation in synovial sarcoma

Thu Jun 01 00:00:00 EDT 2006

Regulation of expression of Bcl-2 protein family member Bim by T cell receptor triggering.

Proceedings of the National Academy of Sciences of the United States of America

Sandalova E,Wei CH,Masucci MG,Levitsky V

13-8800 was used in western blot elucidate Bim regulation in T cells

Tue Mar 02 00:00:00 EST 2004

Targeting and crossing of the human maternofetal barrier by Listeria monocytogenes: role of internalin interaction with trophoblast E-cadherin.

Proceedings of the National Academy of Sciences of the United States of America

Lecuit M,Nelson DM,Smith SD,Khun H,Huerre M,Vacher-Lavenu MC,Gordon JI,Cossart P

Tue Apr 20 00:00:00 EDT 2004

Targeting and crossing of the human maternofetal barrier by Listeria monocytogenes: role of internalin interaction with trophoblast E-cadherin.

Proceedings of the National Academy of Sciences of the United States of America

Lecuit M,Nelson DM,Smith SD,Khun H,Huerre M,Vacher-Lavenu MC,Gordon JI,Cossart P

Tue Apr 20 00:00:00 EDT 2004

Decreased proliferation precedes growth factor changes after physeal irradiation.

Clinical orthopaedics and related research

Damron TA,Horton JA,Naqvi A,Margulies B,Strauss J,Grant W,Farnum CE,Spadaro JA

13-8800 was used in immunohistochemistry to examine the earlier sequential relationship of proliferative, growth factor, and histomorphometric changes after irradiation leading to the 1-week nadir

Sat May 01 00:00:00 EDT 2004

The chemopreventive agent phenethyl isothiocyanate sensitizes cells to Fas-mediated apoptosis.

Carcinogenesis

Pullar JM,Thomson SJ,King MJ,Turnbull CI,Midwinter RG,Hampton MB

Sat May 01 00:00:00 EDT 2004

Temporal changes in PTHrP, Bcl-2, Bax, caspase, TGF-beta, and FGF-2 expression following growth plate irradiation with or without radioprotectant.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Damron TA,Mathur S,Horton JA,Strauss J,Margulies B,Grant W,Farnum CE,Spadaro JA

13-8800 was used in immunohistochemistry to study changes in the expression of apoptosis molecules and growth factors in rats after growth plate irradiation with or without radioprotectant

Sun Feb 01 00:00:00 EST 2004

Oncoprotein profiles of primary peritoneal malignant mixed müllerian tumors.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

Ng JS,Han AC,Edelson MI,Rosenblum NG

13-8800 was used in immunohistochemistry - paraffin section to present three cases of primary peritoneal malignant mixed mullerian tumors

Tue Mar 30 00:00:00 EST 2004

Simultaneous carcinoma of the endometrium and ovary vs endometrial carcinoma with ovarian metastases: a clinical and immunohistochemical determination.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

Halperin R,Zehavi S,Hadas E,Habler L,Bukovsky I,Schneider D

13-8800 was used in immunohistochemistry - paraffin section to compare tumors involving endometrium and ovary and metastatic endometrial tumors

Tue Jun 03 00:00:00 EDT 2003

FKBP12-rapamycin-associated protein associates with mitochondria and senses osmotic stress via mitochondrial dysfunction.

Proceedings of the National Academy of Sciences of the United States of America

Desai BN,Myers BR,Schreiber SL

13-8800 was used in western blot to study the relationship between FKBP12-rapamycin associated protein and mitochondrial dysfunction during osmatic stress.

Tue Apr 02 00:00:00 EST 2002

Microcysts and breast cancer: a study of biological markers in archival biopsy material.

Breast cancer research and treatment

Tran DD,Lawson JS

13-8800 was used in immunohistochemistry - paraffin section to study the contribution of breast cysts to breast cancer

Tue Oct 01 00:00:00 EDT 2002

Lymphomas of the oral cavity: histology, immunologic type, and incidence of Epstein-Barr virus infection.

Human pathology

Solomides CC,Miller AS,Christman RA,Talwar J,Simpkins H

13-8800 was used in immunohistochemistry to study the role of Epstein-Barr virus infection in lymphomas in the nasal cavity.

Fri Feb 01 00:00:00 EST 2002

Evaluation of cell proliferation, epidermal growth factor receptor, and bcl-2 immunoexpression as prognostic factors for patients with World Health Organization grade 2 oligodendroglioma.

Cancer

Reis-Filho JS,Faoro LN,Carrilho C,Bleggi-Torres LF,Schmitt FC

13-8800 was used in immunohistochemistry (paraffin) to assess the immunoexpression of the proliferation index, epidermal growth factor receptor, and bcl-2 as prognostic factors in patients with Grade 2 oligodendroglioma.

Tue Feb 15 00:00:00 EST 2000

Intercellular adhesion molecule 1 underlies the functional heterogeneity of synovial cells in patients with rheumatoid arthritis: involvement of cell cycle machinery.

Arthritis and rheumatism

Tanaka Y,Nomi M,Fujii K,Hübscher S,Maruo A,Matsumoto S,Awazu Y,Saito K,Eto S,Minami Y

13-8800 was used in flow cytometry to separate synovial cells from rheumatoid arthritis synovium based on their proliferative or apoptotic features.

Wed Nov 01 00:00:00 EST 2000

Bioinformatics

Protein Aliases: Apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

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Gene Aliases: AW986256; Bcl-2; BCL2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PPP1R50

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UniProt ID: (Human) P10415, (Mouse) P10417

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Entrez Gene ID: (Human) 596, (Mouse) 12043

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Function(s)

protease binding protein binding transcription factor binding channel activity channel inhibitor activity ubiquitin protein ligase binding identical protein binding protein homodimerization activity sequence-specific DNA binding protein heterodimerization activity BH3 domain binding protein phosphatase 2A binding protein phosphatase binding BH domain binding

Process(es)

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Bcl-2 Monoclonal Antibody (Bcl-2-100)

Bcl-2 Antibody (13-8800) in IHC (P)

Product Details

13-8800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

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