Invitrogen

Bcl-X Monoclonal Antibody (2H12), eBioscience™

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

1 Published Figure
3 References
View all (40) BCL-X antibodies
Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

14-6994-81

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

2H12

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468381

Product Specific Information

Description: The 2H12 antibody reacts with human, mouse and rat Bcl-X. Bcl-X functions as a central repressor of several apoptotic signals.This antibody reacts with both Bcl-XS (short) and Bcl-XL (long) proteins.

Applications Reported: The 2H12 antibody has been reported for use in immunoblotting (WB).

Applications Tested: The 2H12 antibody has been tested by Western blot analysis of HeLa lysate and can be used at less than 2 µg/mL. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded the BCL-X gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: anti-apoptosis regulatory protein; Apoptosis regulator Bcl-X; B cell lymphoma 2 like; B cell lymphoma like X; B-cell leukemia/lymphoma x; Bcl 2 like 1; bcl x; Bcl xL; BCL XL/S; bcl-2 L1; Bcl-2-like protein 1; bcl-xS; Bcl2-L-1; BCL2-like 1; BclxL; BclXS; DKFZp781P2092; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3

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Gene Aliases: Bcl(X)L; Bcl-X; Bcl-XL; BCL-XL/S; bcl2-L-1; BCL2L; BCL2L1; BCLX; Blc2l; PPP1R52

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UniProt ID: (Human) Q07817, (Mouse) Q64373, (Rat) P53563

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Entrez Gene ID: (Human) 598, (Mouse) 12048, (Rat) 24888

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Function(s)
protein binding protein kinase binding identical protein binding protein homodimerization activity protein heterodimerization activity BH3 domain binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process BH domain binding MDM2/MDM4 family protein binding
Process(es)
cytokinesis ovarian follicle development in utero embryonic development release of cytochrome c from mitochondria endocytosis mitotic cell cycle checkpoint germ cell development spermatogenesis cell proliferation positive regulation of cell proliferation male gonad development intrinsic apoptotic signaling pathway in response to DNA damage apoptotic mitochondrial changes fertilization suppression by virus of host apoptotic process response to cytokine growth negative regulation of apoptotic process negative regulation of neuron apoptotic process response to cycloheximide regulation of mitochondrial membrane permeability neuron apoptotic process regulation of mitochondrial membrane potential cellular process regulating host cell cycle in response to virus mitochondrion morphogenesis cellular response to amino acid stimulus cellular response to alkaloid cellular response to gamma radiation apoptotic process in bone marrow negative regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process negative regulation of execution phase of apoptosis negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway response to ischemia apoptotic process response to radiation response to virus regulation of apoptotic process positive regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of anoikis response to hypoxia response to oxidative stress response to inorganic substance response to organonitrogen compound response to lead ion response to organic cyclic compound response to hydrogen peroxide response to peptide hormone response to electrical stimulus
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