Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 6
Bif-1 Antibody (OSE00030W-100UL) in IHC (P)
IHC-P on paraffin sections of rat kidney. The animal was perfused using Autoperfuser at a pressure of 110 mmHg with 300 mL 4% FA and further post fixed overnight before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 µm. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions;... View More
Product Details
OSE00030W-100UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ Ig
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide from human Bif conjugated to blue carrier protein was used as the antigen
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
The peptide is homologous in rat and mouse.
Specificity of this antibody: Bif-1.
Target Information
Bif1 gene encodes a protein similar to a protein in rodents which is induced by bone morphogenic protein 2 in vitro and may be involved in BMP2-induced transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Bax-interacting factor 1; Bif-1; BIF1; endophilin b1; Endophilin-B1; protein phosphatase 1, regulatory subunit 70; SH3 domain-containing GRB2-like protein B1; SH3-containing protein SH3GLB1; SH3-domain GRB2 like endophilin B1; SH3-domain GRB2-like B1 (endophilin); SH3-domain GRB2-like endophilin B1; sh3glb1 {ECO:0000312|EMBL:AAH79085.1}; testicular tissue protein Li 172
Gene Aliases: AA409932; AI314629; AU015566; Bif-1; CGI-61; dJ612B15.2; KIAA0491; mKIAA0491; PPP1R70; SH3GLB1
UniProt ID: (Human) Q9Y371, (Rat) Q6AYE2, (Mouse) Q9JK48
Entrez Gene ID: (Human) 51100, (Rat) 292156, (Mouse) 54673
phosphatidic acid biosynthetic process
apoptotic process
phospholipid biosynthetic process
positive regulation of protein oligomerization
'de novo' posttranslational protein folding
protein oligomerization
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
autophagy
positive regulation of autophagy
regulation of macroautophagy
regulation of protein stability
regulation of cytokinesis
receptor catabolic process
cellular response to amino acid starvation
cellular response to glucose starvation
autophagic cell death
membrane fission
cell-cell adhesion
positive regulation of membrane tubulation
protein localization to vacuolar membrane
positive regulation of protein targeting to mitochondrion
positive regulation of autophagosome assembly
mitochondrion organization
positive regulation of phosphatidylinositol 3-kinase activity
negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support