Invitrogen

BrdU Monoclonal Antibody (BU20A), APC, eBioscience™

FIGURE: 1 / 17

BrdU Antibody (17-5071-42) in Flow

Anti-CD3/CD28 (Product # 16-0031-82, 16-0281)-stimulated mouse splenocytes either unlabeled (left) or labeled with BrdU (right) were surface stained with Anti-Mouse CD4 PE (Product # 12-0041-82). These cells were then stained intracellularly with Anti-BrdU APC using the BrdU Staining Kit for Flow Cytometry APC and protocol. Total viable cells were used for analysis.

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Product Details

17-5071-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 28 publications 28 publications

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

BU20A

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11040534

Product Specific Information

Description: This Bu20a monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU). BrdU is a derivative of uridine that can be incorporated into DNA in place of thymidine during the S-phase of the cell cycle. Anti-BrdU can then be used to identify cells that have undergone DNA synthesis during BrdU treatment.

For staining for flow cytometric analysis, we recommend the use of the BrdU Staining Buffer Set (Product # 00-5525-00) and protocol.

Applications Reported: This BU20A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This BU20A antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of BrdU-labeled mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol or the BrdU Staining Buffer Set (Product # 00-5525-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

BrdU labeling and staining with the Anti-BrdU antibody:1. Label dividing cells with 10 µM BrdU for 45 min at 37°C.2. Following the incubation, harvest the cells and wash once with 1X PBS.3. Stain surface molecules according to the Surface Staining Protocol.4. Wash in cold Flow Cytometry Staining Buffer or 1X PBS.5. Resuspend the cell pellet by pulse vortexing. Then add 1 mL of freshly prepared Foxp3 Fixation/Permeabilization Buffer (Product # 00-5521-00) to each sample. pulse vortex again.6. Incubate for 30 to 60 minutes at 2-8°C in the dark.7. Wash once with cold Flow Cytometry Staining Buffer followed by centrifugation. Decant the supernatant.8. Resuspend the cell pellet with 100 µL Flow Cytometry Staining Buffer containing 30 µg of Dnase I.9. Incubate for 1 hr at 37°C and then wash.10. Stain cells with anti-BrdU antibody for 30 min to 1 hr and then wash.10. Analyze the samples.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

DOT1L Is a Novel Cancer Stem Cell Target for Triple-Negative Breast Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Kurani H,Razavipour SF,Harikumar KB,Dunworth M,Ewald AJ,Nasir A,Pearson G,Van Booven D,Zhou Z,Azzam D,Wahlestedt C,Slingerland J

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Mon May 02 00:00:00 EDT 2022

Astrocytic interleukin-3 programs microglia and limits Alzheimer's disease.

Nature

McAlpine CS,Park J,Griciuc A,Kim E,Choi SH,Iwamoto Y,Kiss MG,Christie KA,Vinegoni C,Poller WC,Mindur JE,Chan CT,He S,Janssen H,Wong LP,Downey J,Singh S,Anzai A,Kahles F,Jorfi M,Feruglio PF,Sadreyev RI,Weissleder R,Kleinstiver BP,Nahrendorf M,Tanzi RE,Swirski FK

17-5071-42 was used in Flow cytometry/Cell sorting to identify IL-3 as a key mediator of astrocyte-microglia cross-talk and a node for therapeutic intervention in Alzheimer's disease.

Thu Jul 01 00:00:00 EDT 2021

Gain-of-function hot spot mutant p53^R248Q regulation of integrin/FAK/ERK signaling in esophageal squamous cell carcinoma.

Translational oncology

Yu VZ,So SS,Lung ML

17-5071 was used in Flow cytometry/Cell sorting to identify a novel GoF mechanism through which a specific p53 mutant exerts oncogenic features and contributes to ESCC tumorigenesis.

Fri Jan 01 00:00:00 EST 2021

Gain-of-function hot spot mutant p53^R248Q regulation of integrin/FAK/ERK signaling in esophageal squamous cell carcinoma.

Translational oncology

Yu VZ,So SS,Lung ML

17-5071 was used in Flow cytometry/Cell sorting to identify a novel GoF mechanism through which a specific p53 mutant exerts oncogenic features and contributes to ESCC tumorigenesis.

Fri Jan 01 00:00:00 EST 2021

BCOR Internal Tandem Duplication Expression in Neural Stem Cells Promotes Growth, Invasion, and Expression of PRC2 Targets.

International journal of molecular sciences

Nakata S,Yuan M,Rubens JA,Kahlert UD,Maciaczyk J,Raabe EH,Eberhart CG

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Sat Apr 10 00:00:00 EDT 2021

IgA Plasma Cells Are Long-Lived Residents of Gut and Bone Marrow That Express Isotype- and Tissue-Specific Gene Expression Patterns.

Frontiers in immunology

Wilmore JR,Gaudette BT,Gómez Atria D,Rosenthal RL,Reiser SK,Meng W,Rosenfeld AM,Luning Prak ET,Allman D

17-5071-42 was used in Flow Cytometry to suggest that systemic and mucosal IgA+ plasma cells are from a common source, but they do not migrate between tissues.

Wed Feb 16 00:00:00 EST 2022

PD-L1 recruits phospholipase C and enhances tumorigenicity of lung tumors harboring mutant forms of EGFR.

Cell reports

Ghosh S,Nataraj NB,Noronha A,Patkar S,Sekar A,Mukherjee S,Winograd-Katz S,Kramarski L,Verma A,Lindzen M,Garcia DD,Green J,Eisenberg G,Gil-Henn H,Basu A,Lender Y,Weiss S,Oren M,Lotem M,Geiger B,Ruppin E,Yarden Y

17-5071-42 was used in Immunocytochemistry to portray PD-L1 as a molecular amplifier of EGFR signaling and improve the understanding of the resistance of EGFR+ tumors to immunotherapy.

Tue May 25 00:00:00 EDT 2021

Detecting chromatin interactions between and along sister chromatids with SisterC.

Nature methods

Oomen ME,Hedger AK,Watts JK,Dekker J

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Myofiber necroptosis promotes muscle stem cell proliferation via releasing Tenascin-C during regeneration.

Cell research

Zhou S,Zhang W,Cai G,Ding Y,Wei C,Li S,Yang Y,Qin J,Liu D,Zhang H,Shao X,Wang J,Wang H,Yang W,Wang H,Chen S,Hu P,Sun L

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Insulin-Like Growth Factors Are Key Regulators of T Helper 17 Regulatory T Cell Balance in Autoimmunity.

Immunity

DiToro D,Harbour SN,Bando JK,Benavides G,Witte S,Laufer VA,Moseley C,Singer JR,Frey B,Turner H,Bruning J,Darley-Usmar V,Gao M,Conover C,Hatton RD,Frank S,Colonna M,Weaver CT

17-5071 was used in Flow cytometry/Cell sorting to show that, among effector T cell subsets, Th17 and Treg cells selectively expressed multiple components of the IGF system.

Tue Apr 14 00:00:00 EDT 2020

Prostate Epithelial RON Signaling Promotes M2 Macrophage Activation to Drive Prostate Tumor Growth and Progression.

Molecular cancer research : MCR

Sullivan C,Brown NE,Vasiliauskas J,Pathrose P,Starnes SL,Waltz SE

17-5071 was used in Flow cytometry/Cell sorting to provide additional evidence that epithelial RON expression coordinates interactions between prostate tumor cells and macrophages to promote macrophage-mediated tumor cell growth.

Sat Aug 01 00:00:00 EDT 2020

Autotaxin inhibition reduces cardiac inflammation and mitigates adverse cardiac remodeling after myocardial infarction.

Journal of molecular and cellular cardiology

Tripathi H,Al-Darraji A,Abo-Aly M,Peng H,Shokri E,Chelvarajan L,R Donahue R,Levitan BM,Gao E,Hernandez G,Morris AJ,Smyth SS,Abdel-Latif A

17-5071-42 was used in Flow Cytometry to study the autotaxin activity and lysophosphatidic acid levels in relation to cardiac and systemic inflammation in acute myocardial infarction patients and C57BL/6 (WT) mice.

Tue Dec 01 00:00:00 EST 2020

ASH2L drives proliferation and sensitivity to bleomycin and other genotoxins in Hodgkin's lymphoma and testicular cancer cells.

Cell death & disease

Constantin D,Widmann C

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Mon Nov 30 00:00:00 EST 2020

Differential expansion of T central memory precursor and effector subsets is regulated by division speed.

Nature communications

Kretschmer L,Flossdorf M,Mir J,Cho YL,Plambeck M,Treise I,Toska A,Heinzel S,Schiemann M,Busch DH,Buchholz VR

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Wed Jan 08 00:00:00 EST 2020

Sleep modulates haematopoiesis and protects against atherosclerosis.

Nature

McAlpine CS,Kiss MG,Rattik S,He S,Vassalli A,Valet C,Anzai A,Chan CT,Mindur JE,Kahles F,Poller WC,Frodermann V,Fenn AM,Gregory AF,Halle L,Iwamoto Y,Hoyer FF,Binder CJ,Libby P,Tafti M,Scammell TE,Nahrendorf M,Swirski FK

17-5071-42 was used in Flow Cytometry to investigate the cellular and molecular mechanisms by which sleep maintains cardiovascular health.

Fri Feb 01 00:00:00 EST 2019

The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice.

eLife

Schmidt K,Zhang Q,Tasdogan A,Petzold A,Dahl A,Arneth BM,Slany R,Fehling HJ,Kranz A,Stewart AF,Anastassiadis K

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

FOXP1 controls mesenchymal stem cell commitment and senescence during skeletal aging.

The Journal of clinical investigation

Li H,Liu P,Xu S,Li Y,Dekker JD,Li B,Fan Y,Zhang Z,Hong Y,Yang G,Tang T,Ren Y,Tucker HO,Yao Z,Guo X

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Mon Apr 03 00:00:00 EDT 2017

Loss of Forkhead box M1 promotes erythropoiesis through increased proliferation of erythroid progenitors.

Haematologica

Youn M,Wang N,LaVasseur C,Bibikova E,Kam S,Glader B,Sakamoto KM,Narla A

17-5071 was used in Flow cytometry/Cell sorting to find that inhibition of FOXM1, forkhead domain inhibitor-6, increases red blood cell numbers through additional proliferation of erythroid precursors.

Mon May 01 00:00:00 EDT 2017

4PD Functionalized Dendrimers: A Flexible Tool for In Vivo Gene Silencing of Tumor-Educated Myeloid Cells.

Journal of immunology (Baltimore, Md. : 1950)

Zilio S,Vella JL,De la Fuente AC,Daftarian PM,Weed DT,Kaifer A,Marigo I,Leone K,Bronte V,Serafini P

17-5071 was used in Flow cytometry/Cell sorting to study the use of 4PD nanoparticles for the in vivo gene silencing of tumour-educated myeloid cells.

Mon May 15 00:00:00 EDT 2017

PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts.

Genes to cells : devoted to molecular & cellular mechanisms

Danno S,Kubouchi K,Mehruba M,Abe M,Natsume R,Sakimura K,Eguchi S,Oka M,Hirashima M,Yasuda H,Mukai H

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Downregulation of ZBTB24 hampers the G0/1- to S-phase cell-cycle transition via upregulating the expression of IRF-4 in human B cells.

Genes and immunity

Liang J,Yan R,Chen G,Feng J,Wu WW,Ren W,Zhu C,Zhao Y,Gao XM,Wang J

17-5071-42 was used in Flow Cytometry to show that downregulation of ZBTB24 hampers the G0/1- to S-phase cell-cycle transition via upregulating the expression of IRF-4 in human B cells.

Fri Jul 01 00:00:00 EDT 2016

Breast-cancer-secreted miR-122 reprograms glucose metabolism in premetastatic niche to promote metastasis.

Nature cell biology

Fong MY,Zhou W,Liu L,Alontaga AY,Chandra M,Ashby J,Chow A,O'Connor ST,Li S,Chin AR,Somlo G,Palomares M,Li Z,Tremblay JR,Tsuyada A,Sun G,Reid MA,Wu X,Swiderski P,Ren X,Shi Y,Kong M,Zhong W,Chen Y,Wang SE

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Sun Feb 01 00:00:00 EST 2015

Functional genomic screening reveals asparagine dependence as a metabolic vulnerability in sarcoma.

eLife

Hettmer S,Schinzel AC,Tchessalova D,Schneider M,Parker CL,Bronson RT,Richards NG,Hahn WC,Wagers AJ

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Sat Oct 24 00:00:00 EDT 2015

CENP-32 is required to maintain centrosomal dominance in bipolar spindle assembly.

Molecular biology of the cell

Ohta S,Wood L,Toramoto I,Yagyu K,Fukagawa T,Earnshaw WC

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Programmed death 1 regulates memory phenotype CD4 T cell accumulation, inhibits expansion of the effector memory phenotype subset and modulates production of effector cytokines.

PloS one

Charlton JJ,Tsoukatou D,Mamalaki C,Chatzidakis I

17-5071 was used in Flow cytometry/Cell sorting to demonstrate that PD-1 has an important role in determining the composition and functional aspects of the memory phenotype CD4 T cell pool.

Wed Apr 13 00:00:00 EDT 2016

Atm deletion with dual recombinase technology preferentially radiosensitizes tumor endothelium.

The Journal of clinical investigation

Moding EJ,Lee CL,Castle KD,Oh P,Mao L,Zha S,Min HD,Ma Y,Das S,Kirsch DG

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Fri Aug 01 00:00:00 EDT 2014

Interleukin-7 expression and its effect on natural killer cells in patients with multiple sclerosis.

Journal of neuroimmunology

Su N,Shi SX,Zhu X,Borazanci A,Shi FD,Gan Y

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Sat Nov 15 00:00:00 EST 2014

MiR-630 inhibits proliferation by targeting CDC7 kinase, but maintains the apoptotic balance by targeting multiple modulators in human lung cancer A549 cells.

Cell death & disease

Cao JX,Lu Y,Qi JJ,An GS,Mao ZB,Jia HT,Li SY,Ni JH

Published figure using BrdU monoclonal antibody (Product # 17-5071-42) in Flow Cytometry

Thu Sep 25 00:00:00 EDT 2014

Soluble factors secreted by T cells promote β-cell proliferation.

Diabetes

Dirice E,Kahraman S,Jiang W,El Ouaamari A,De Jesus DF,Teo AK,Hu J,Kawamori D,Gaglia JL,Mathis D,Kulkarni RN

17-5071 was used in Immunohistochemistry to study the role of soluble factors secreted from T cells to enhance pancreatic β-cell proliferation.

Wed Jan 01 00:00:00 EST 2014

Ponatinib suppresses the development of myeloid and lymphoid malignancies associated with FGFR1 abnormalities.

Leukemia

Ren M,Qin H,Ren R,Cowell JK

17-5071 was used in Flow cytometry/Cell sorting to investigate the use of ponatinib for treatment of myeloid and lymphoid malignancies.

Tue Jan 01 00:00:00 EST 2013

Selective reduction of post-selection CD8 thymocyte proliferation in IL-15Rα deficient mice.

PloS one

Chow KP,Qiu JT,Lee JM,Hsu SL,Yang SC,Wu NN,Huang W,Wu TS

17-5071 was used in Flow cytometry/Cell sorting to investigate the unique IL-15-dependent cell expansion at the mature CD8SP stage of thymocyte development.

Mon Aug 20 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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BrdU Monoclonal Antibody (BU20A), APC, eBioscience™

BrdU Antibody (17-5071-42) in Flow

Product Details

17-5071-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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