Invitrogen

BrdU Monoclonal Antibody (BU20A)

FIGURE: 1 / 4

BrdU Antibody (MA1-81890) in Flow

Flow cytometric analysis of BrdU incorporation into pulsed-labelled ACNC12 cells using a Bromodeoxyuridine monoclonal antibody (Product # MA1-81890).

BrdU Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-81890

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:25-1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

1:25-1:100

Product Specifications

Species Reactivity

Chemical

Published species

Chemical, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

BU20A

Immunogen

Bromodeoxyuridine conjugated to BSA.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_927209

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti BrdU antibody, clone Bu20a recognizes bromodeoxyuridine (known as BrdU or BrdUrd).

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Translation of Tudor-SN, a novel terminal oligo-pyrimidine (TOP) mRNA, is regulated by the mTORC1 pathway in cardiomyocytes.

RNA biology

Gan S,Su C,Ma J,Liu M,Cui X,Xin L,Ren Y,Gao X,Ge L,Wei M,Yang J

MA1-81890 was used in Immunocytochemistry to suggest that the translational efficiency of Tudor-SN is regulated by the mTORC1 pathway in myocardia and that Tudor-SN is involved in mTORC1-mediated regulation of cardiomyocytic proliferation and cardiac development.

Tue Jun 01 00:00:00 EDT 2021

Intestinal Adaptation upon Chemotherapy-Induced Intestinal Injury in Mice Depends on GLP-2 Receptor Activation.

Biomedicines

Billeschou A,Hunt JE,Ghimire A,Holst JJ,Kissow H

Published figure using BrdU monoclonal antibody (Product # MA1-81890) in Immunohistochemistry

Thu Jan 07 00:00:00 EST 2021

Pharmacological activation of TGR5 promotes intestinal growth via a GLP-2-dependent pathway in mice.

American journal of physiology. Gastrointestinal and liver physiology

Hunt JE,Billeschou A,Windeløv JA,Hartmann B,Ullmer C,Holst JJ,Kissow H

MA1-81890 was used in Immunohistochemistry to investigate the consequences of pharmacological TGR5 receptor activation on L cell hormone production in vivo using the specific TGR5 agonist RO5527239 and the GLP-2 receptor knockout mouse.

Fri May 01 00:00:00 EDT 2020

Hyperglycemia and T Cell infiltration are associated with stromal and epithelial prostatic hyperplasia in the nonobese diabetic mouse.

The Prostate

Aaron-Brooks LM,Sasaki T,Vickman RE,Wei L,Franco OE,Ji Y,Crawford SE,Hayward SW

MA1-81890 was used in Immunohistochemistry to investigate the non-obese diabetic (NOD) mouse as a potential model for subsequent intervention studies.

Sat Jun 01 00:00:00 EDT 2019

DGAT1 Inhibitor Suppresses Prostate Tumor Growth and Migration by Regulating Intracellular Lipids and Non-Centrosomal MTOC Protein GM130.

Scientific reports

Nardi F,Franco OE,Fitchev P,Morales A,Vickman RE,Hayward SW,Crawford SE

Published figure using BrdU monoclonal antibody (Product # MA1-81890) in Immunohistochemistry

Thu Feb 28 00:00:00 EST 2019

Exogenous glucagon-like peptide-2 (GLP-2) prevents chemotherapy-induced mucositis in rat small intestine.

Cancer chemotherapy and pharmacology

Kissow H,Viby NE,Hartmann B,Holst JJ,Timm M,Thim L,Poulsen SS

MA1-81890 was used in immunohistochemistry to study the effect of exogenous glucagon-like peptide-2 on chemotherapy-induced mucositis in rat small intestine

Sun Jul 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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