Invitrogen

BrdU Monoclonal Antibody (MoBU-1)

BrdU Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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BrdU Antibody (MA1-19213) in IHC (P)

Immunohistochemistry staining of bromodeoxyuridine-labeled cells (chick embryo; paraffin-embedded sections) with anti-5-bromodeoxyuridine (MoBu-1) Monoclonal antibody (Product # MA1-19213).

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Product Details

MA1-19213

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

1-2 µg/mL

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MoBU-1

Immunogen

BrdU conjugated to hemocyanine.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1070849

Product Specific Information

This antibody reacts also specifically with 5-bromouridine (BrU), and it does not cross-react with EdU.

Target Information

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Polo-like kinase 4 inhibitor CFI-400945 inhibits carotid arterial neointima formation but increases atherosclerosis.

Cell death discovery

Sun J,Belke D,Gui Y,Chen YX,Zhou S,Zheng XL

MA1-19213 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin) to show that CFI-400945 prevents carotid arterial neointima formation in C57BL/6 mice but accelerates atherosclerosis in ApoE-/- mice, likely through mitotic arrest and subsequent induction of polyploidization and apoptosis.

Tue Feb 07 00:00:00 EST 2023

Age and prior exercise in vivo determine the subsequent in vitro molecular profile of myoblasts and nonmyogenic cells derived from human skeletal muscle.

American journal of physiology. Cell physiology

Bechshøft CJL,Jensen SM,Schjerling P,Andersen JL,Svensson RB,Eriksen CS,Mkumbuzi NS,Kjaer M,Mackey AL

MA1-19213 was used in Immunohistochemistry to show that the in vitro phenotype and molecular profile of human skeletal muscle myoblasts and fibroblasts is determined by the age and exercise state of the original in vivo environment.

Sat Jun 01 00:00:00 EDT 2019

Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model.

Biomaterials

Lo CY,Weil BR,Palka BA,Momeni A,Canty JM,Neelamegham S

MA1-19213 was used in flow cytometry to discuss and investigate methods of cellular glycoengineering.

Fri Jan 01 00:00:00 EST 2016

The Periodontal Pathogen Porphyromonas gingivalis Preferentially Interacts with Oral Epithelial Cells in S Phase of the Cell Cycle.

Infection and immunity

Al-Taweel FB,Douglas CWI,Whawell SA

MA119213 was used in immunocytochemistry to investigate the effect of oral keratinocyte cell cycle on Porphyromonas gingivalis infection

Fri Jul 01 00:00:00 EDT 2016

MERIT40 cooperates with BRCA2 to resolve DNA interstrand cross-links.

Genes & development

Jiang Q,Paramasivam M,Aressy B,Wu J,Bellani M,Tong W,Seidman MM,Greenberg RA

MA1-19213 was used in flow cytometry to study resolution of DNA interstrand cross-links by cooperation of BRCA2 and MERIT40

Tue Sep 15 00:00:00 EDT 2015

Tumor suppressor and deubiquitinase BAP1 promotes DNA double-strand break repair.

Proceedings of the National Academy of Sciences of the United States of America

Yu H,Pak H,Hammond-Martel I,Ghram M,Rodrigue A,Daou S,Barbour H,Corbeil L,Hébert J,Drobetsky E,Masson JY,Di Noia JM,Affar el B

MA1-19213 was used in flow cytometry to determine deubiquitinases required to assemble homologous recombination factors BRCA1 and RAD51

Tue Jan 07 00:00:00 EST 2014

Over-inhibition of NADPH oxidase reduce the wound healing in liver of finfish.

Fish & shellfish immunology

Wu YS,Huang SL,Nan FH,Chang CS,Hsiao CM,Lai KC,Chen SN

MA1-19213 was used in immunohistochemistry - paraffin section to study the ROS produced by injury using zebrafish liver cell and tilapia partial hepatectomy models

Mon Sep 01 00:00:00 EDT 2014

Profiling dose-dependent activation of p53-mediated signaling pathways by chemicals with distinct mechanisms of DNA damage.

Toxicological sciences : an official journal of the Society of Toxicology

Clewell RA,Sun B,Adeleye Y,Carmichael P,Efremenko A,McMullen PD,Pendse S,Trask OJ,White A,Andersen ME

MA1-19213 was used in flow cytometry to develop high throughput methods to assess proof-of-concept in vitro-only risk assessments

Sat Nov 01 00:00:00 EDT 2014

Notch3 signaling gates cell cycle entry and limits neural stem cell amplification in the adult pallium.

Development (Cambridge, England)

Alunni A,Krecsmarik M,Bosco A,Galant S,Pan L,Moens CB,Bally-Cuif L

MA1-19213 was used in immunohistochemistry (free floating) to examine the role of Notch3 in neuronal stem cells.

Thu Aug 01 00:00:00 EDT 2013

Bioinformatics

Protein Aliases: 5-Bromo-2-deoxyuridine; Bromodeoxyuridine

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BrdU Monoclonal Antibody (MoBU-1)

BrdU Antibody (MA1-19213) in IHC (P)

Product Details

MA1-19213

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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