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C/EBP alpha Antibody (PA5-77911) in WB
Western Blot using C/EBP alpha Polyclonal Antibody (Product # PA5-77911). Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha Polyclonal Antibody (Product # PA5-77911) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Product Details
PA5-77911
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Bovine
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human C/EBP alpha. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.59 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2735541
Product Specific Information
Positive Control: THP-1, HL-60, C2C12, HepG2, NB4, U937, THP-1, MV4-11
Predicted Reactivity: Rat (100%), Sheep (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C EBP; C/EBP alpha; CCAAT/enhancer binding protein (C/EBP), alpha; CCAAT/enhancer-binding protein alpha; OTTHUMP00000220269
Gene Aliases: C/EBP-alpha; C/ebpalpha; CBF-A; CEBP; CEBPA
UniProt ID: (Human) P49715, (Mouse) P53566
Entrez Gene ID: (Human) 1050, (Mouse) 12606
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
protein binding
transcription factor binding
kinase binding
protein homodimerization activity
transcription regulatory region DNA binding
RNA polymerase I regulatory region DNA binding
protein domain specific binding
protein complex binding
histone deacetylase binding
sequence-specific DNA binding
protein heterodimerization activity
HMG box domain binding
basic leucine zipper transcription factor
urea cycle
negative regulation of transcription from RNA polymerase II promoter
liver development
embryonic placenta development
generation of precursor metabolites and energy
transcription, DNA-templated
transcription from RNA polymerase II promoter
mitochondrion organization
Notch signaling pathway
cholesterol metabolic process
negative regulation of cell proliferation
viral process
cytokine-mediated signaling pathway
myeloid cell differentiation
macrophage differentiation
lung development
granulocyte differentiation
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
glucose homeostasis
fat cell differentiation
positive regulation of fat cell differentiation
positive regulation of osteoblast differentiation
negative regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase III promoter
cell maturation
inner ear development
white fat cell differentiation
brown fat cell differentiation
lipid homeostasis
cellular response to lithium ion
cellular response to organic cyclic compound
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase I promoter
multicellular organism development
regulation of cell proliferation
negative regulation of cell cycle
positive regulation of transcription, DNA-templated
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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