Invitrogen

C/EBP beta Monoclonal Antibody (1H7)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

8 References

View all (25) C/EBP beta antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

C/EBP beta Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 4

C/EBP beta Antibody (MA1-827) in ICC/IF

Immunofluorescence staining of C/EBP beta in P388D1 cells using Product # MA1-827.

C/EBP beta Antibody in Western Blot (WB)

C/EBP beta Antibody in Immunoprecipitation (IP)

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Product Details

MA1-827

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

4 µg/mL

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

ChIP assay (ChIP)

View 1 publication 1 publication

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1H7

Immunogen

Recombinant mouse LIP.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2078054

Product Specific Information

MA1-827 detects C/EBP beta protein in mouse and rat samples. MA1-827 detects the LAP*, LAP and LIP isoforms of C/EBP beta.

MA1-827 has successfully been used in Western blot, immunofluorescent and immunoprecipitation procedures. By Western blot, this antibody detects 38, 35 and 20 kDa proteins representing C/EBP beta LAP*, LAP, and LIP isoforms respectively, in 3T3-L1 cells. LIP is recognized as the C-terminus fragment. Immunofluorescent staining of C/EBP beta in P388D1 cells can be performed using MA1-827

The MA1-827 immunogen is recombinant mouse LIP.

Target Information

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Cancer-Induced Muscle Wasting Requires p38β MAPK Activation of p300.

Cancer research

Sin TK,Zhang G,Zhang Z,Zhu JZ,Zuo Y,Frost JA,Li M,Li YP

MA1-827 was used in Western Blot to understand the role of p300 in the underlying mechanism behind cancer-associated cachexia.

Mon Feb 15 00:00:00 EST 2021

p300 Mediates Muscle Wasting in Lewis Lung Carcinoma.

Cancer research

Sin TK,Zhu JZ,Zhang G,Li YP

MA1-827 was used in Immunoprecipitation to demonstrate p300 as an indispensable mediator of Lewis lung carcinoma tumor-induced muscle wasting due to its activation of C/EBPβ by acetylating Lys39.

Mon Apr 01 00:00:00 EDT 2019

p300 Mediates Muscle Wasting in Lewis Lung Carcinoma.

Cancer research

Sin TK,Zhu JZ,Zhang G,Li YP

MA1-827 was used in Immunoprecipitation to demonstrate p300 as an indispensable mediator of Lewis lung carcinoma tumor-induced muscle wasting due to its activation of C/EBPβ by acetylating Lys39.

Mon Apr 01 00:00:00 EDT 2019

BHLHE40, a third transcription factor required for insulin induction of SREBP-1c mRNA in rodent liver.

eLife

Tian J,Wu J,Chen X,Guo T,Chen ZJ,Goldstein JL,Brown MS

Published figure using C/EBP beta monoclonal antibody (Product # MA1-827) in Immunoprecipitation

Wed Jun 27 00:00:00 EDT 2018

Cutting Edge: Synchronization of IRF1, JunB, and C/EBPβ Activities during TLR3-TLR7 Cross-Talk Orchestrates Timely Cytokine Synergy in the Proinflammatory Response.

Journal of immunology (Baltimore, Md. : 1950)

Liu Q,Zhu Y,Yong WK,Sze NS,Tan NS,Ding JL

Published figure using C/EBP beta monoclonal antibody (Product # MA1-827) in Western Blot

Sat Aug 01 00:00:00 EDT 2015

Glutamine-mediated dual regulation of heat shock transcription factor-1 activation and expression.

The Journal of biological chemistry

Xue H,Slavov D,Wischmeyer PE

MA1-827 was used in EMSA and western blot to study the mechanisms by which glutamine regulates HSF-1 expression and transactivation

Fri Nov 23 00:00:00 EST 2012

Glutamine-mediated dual regulation of heat shock transcription factor-1 activation and expression.

The Journal of biological chemistry

Xue H,Slavov D,Wischmeyer PE

MA1-827 was used in EMSA and western blot to study the mechanisms by which glutamine regulates HSF-1 expression and transactivation

Fri Nov 23 00:00:00 EST 2012

Differential activation of a C/EBP beta isoform by a novel redox switch may confer the lipopolysaccharide-inducible expression of interleukin-6 gene.

The Journal of biological chemistry

Su WC,Chou HY,Chang CJ,Lee YM,Chen WH,Huang KH,Lee MY,Lee SC

MA1-827 was used in chimmunoprecipitation to investigate the relationship between the transcriptional activities of different C/EBP beta isoforms and the regulation of interleukin 6 gene expression

Fri Dec 19 00:00:00 EST 2003

Bioinformatics

Protein Aliases: AGP/EBP; C EBP; C/EBP BETA; C/EBP-related protein 2; CCAAT/enhancer-binding protein beta; IL-6DBP; Interleukin-6-dependent-binding protein; LAP; LIP; Liver-enriched inhibitory protein; Liver-enriched transcriptional activator; nuclear factor-IL6; nuclear protein Il6; OTTMUSP00000017503; SF-B; Silencer factor B

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Gene Aliases: C/EBPbeta; Cebpb; CRP2; IL-6DBP; Il6dbp; LAP; LIP; NF-IL6; NF-M; Nfil6; Sfb; TCF5

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UniProt ID: (Mouse) P28033, (Rat) P21272

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Entrez Gene ID: (Mouse) 12608, (Rat) 24253

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding kinase binding histone acetyltransferase binding glucocorticoid receptor binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding ubiquitin-like protein ligase binding protein heterodimerization activity basic leucine zipper transcription factor

Process(es)

ovarian follicle development embryonic placenta development granuloma formation transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter cell differentiation neuron differentiation response to lipopolysaccharide positive regulation of interleukin-4 production mammary gland epithelial cell proliferation response to endoplasmic reticulum stress T-helper 1 cell activation negative regulation of T cell proliferation defense response to bacterium negative regulation of neuron apoptotic process regulation of interleukin-6 biosynthetic process fat cell differentiation positive regulation of fat cell differentiation positive regulation of osteoblast differentiation regulation of osteoclast differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter brown fat cell differentiation mammary gland epithelial cell differentiation regulation of transcription involved in cell fate commitment intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to amino acid stimulus hepatocyte proliferation liver regeneration positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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C/EBP beta Monoclonal Antibody (1H7)

C/EBP beta Antibody (MA1-827) in ICC/IF

Product Details

MA1-827

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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