Proteintech

C5aR Polyclonal Antibody

C5aR Antibody in Immunocytochemistry (ICC/IF)

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C5aR Antibody (21316-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using C5AR1 antibody 21316-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

C5aR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

C5aR Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

21316-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 8 publications 8 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

C5aR Fusion Protein Ag15968 (251-350 aa encoded by BC008982)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FFIFWLPYQ VTGIMMSFLE PSSPTFLLLN KLDSLCVSFA YINCCINPII YVVAGQGFQG RLRKSLPSLL RNVLTEESVV RESKSFTRST VDTMAQKTQA V (251-350 aa encoded by BC008982)

Target Information

CD88, also known as C5aR1, is a C5a Anaphylatoxin Receptor that mediates the effects of one of the most potent chemotactic agents known. C5AR1 has been implicated in asthma, and its expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. CD88 is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. Functionally, CD88 stimulates chemotaxis, granule enzyme release and superoxide anion production. CD88 is also a component of the classical pathway of the complement cascade; it may be involved with tissue injury associated with ischemia or sepsis and it may be a component of the membrane attack complex. Diseases associated with CD88 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

C5aR antagonist inhibits LPS-induced inflammation in human gingival fibroblasts via NF-κB and MAPK signaling pathways.

Journal of applied oral science : revista FOB

Chen Y,Liu Y,Li H,Huna R,Tan X,Li N,Zhang Y,Jiao X,Liu M

Fri Feb 10 00:00:00 EST 2023

Determination of the optimal concentration and duration of C5aR antagonist application in an inflammatory model of human dental pulp cells.

FEBS open bio

Hu J,Tan X,Wei X,Hu W,Gao L,Cao X,Yang H,Jiang Z,Li N,Teng L,Liu M

Wed Mar 01 00:00:00 EST 2023

C5aR1 promotes the progression of colorectal cancer by EMT and activating Wnt/β-catenin pathway.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico

Xu D,Li M,Ran L,Li X,Sun X,Yin T

Wed Feb 01 00:00:00 EST 2023

Complement C5aR/LPS-induced BDNF and NGF modulation in human dental pulp stem cells.

Scientific reports

Irfan M,Kim JH,Druzinsky RE,Ravindran S,Chung S

Mon Feb 07 00:00:00 EST 2022

The role of complement C5a receptor in DPSC odontoblastic differentiation and in vivo reparative dentin formation.

International journal of oral science

Irfan M,Kim JH,Marzban H,Reed DA,George A,Cooper LF,Chung S

Thu Jan 27 00:00:00 EST 2022

QiShenYiQi Pill Ameliorates Cardiac Fibrosis After Pressure Overload-Induced Cardiac Hypertrophy by Regulating FHL2 and the Macrophage RP S19/TGF-β1 Signaling Pathway.

Frontiers in pharmacology

Anwaier G,Xie TT,Pan CS,Li AQ,Yan L,Wang D,Chen FK,Weng DZ,Sun K,Chang X,Fan JY,Han JY,Liu J

Tue Aug 02 00:00:00 EDT 2022

QiShenYiQi Pill Ameliorates Cardiac Fibrosis After Pressure Overload-Induced Cardiac Hypertrophy by Regulating FHL2 and the Macrophage RP S19/TGF-β1 Signaling Pathway.

Frontiers in pharmacology

Anwaier G,Xie TT,Pan CS,Li AQ,Yan L,Wang D,Chen FK,Weng DZ,Sun K,Chang X,Fan JY,Han JY,Liu J

Tue Aug 02 00:00:00 EDT 2022

Suppressing MDSC Infiltration in Tumor Microenvironment Serves as an Option for Treating Ovarian Cancer Metastasis.

International journal of biological sciences

Li Y,Zhang Q,Wu M,Zhang P,Huang L,Ai X,Yang Z,Shen Q,Wang Y,Wang P,Zhou S,He ML

Tue Jul 12 00:00:00 EDT 2022

Suppressing MDSC Infiltration in Tumor Microenvironment Serves as an Option for Treating Ovarian Cancer Metastasis.

International journal of biological sciences

Li Y,Zhang Q,Wu M,Zhang P,Huang L,Ai X,Yang Z,Shen Q,Wang Y,Wang P,Zhou S,He ML

Tue Jul 12 00:00:00 EDT 2022

HSP90-Mediates Liraglutide Preconditioning-Induced Cardioprotection by Inhibiting C5a and NF-κB.

Journal of investigative surgery : the official journal of the Academy of Surgical Research

He ST,Wang DX,Meng JJ,Cheng XF,Bi Q,Zhong GQ,Tu RH

Sun May 01 00:00:00 EDT 2022

Activation of C3 and C5 May Be Involved in the Inflammatory Progression of PCM and GM.

Inflammation

Li XQ,Sun HG,Wang XH,Zhang HJ,Zhang XS,Yu Y,Liu J,Guo QQ,Yang ZL

Fri Apr 01 00:00:00 EDT 2022

Activation of C3 and C5 May Be Involved in the Inflammatory Progression of PCM and GM.

Inflammation

Li XQ,Sun HG,Wang XH,Zhang HJ,Zhang XS,Yu Y,Liu J,Guo QQ,Yang ZL

Fri Apr 01 00:00:00 EDT 2022

Inhibition of the immunoproteasome modulates innate immunity to ameliorate muscle pathology of dysferlin-deficient BlAJ mice.

Cell death & disease

Farini A,Tripodi L,Villa C,Napolitano F,Strati F,Molinaro D,Facciotti F,Cassani B,Torrente Y

Sat Nov 19 00:00:00 EST 2022

Inhibition of the immunoproteasome modulates innate immunity to ameliorate muscle pathology of dysferlin-deficient BlAJ mice.

Cell death & disease

Farini A,Tripodi L,Villa C,Napolitano F,Strati F,Molinaro D,Facciotti F,Cassani B,Torrente Y

Sat Nov 19 00:00:00 EST 2022

C5a complement receptor modulates odontogenic dental pulp stem cell differentiation under hypoxia.

Connective tissue research

Pasiewicz R,Valverde Y,Narayanan R,Kim JH,Irfan M,Lee NS,George A,Cooper LF,Alapati SB,Chung S

Fri Jul 01 00:00:00 EDT 2022

Complement Activation and Organ Damage After Trauma-Differential Immune Response Based on Surgical Treatment Strategy.

Frontiers in immunology

Lackner I,Weber B,Baur M,Fois G,Gebhard F,Pfeifer R,Cinelli P,Halvachizadeh S,Lipiski M,Cesarovic N,Schrezenmeier H,Huber-Lang M,Pape HC,Kalbitz M

Mon Apr 05 00:00:00 EDT 2021

Linking Complement C3 and B Cells in Nasal Polyposis.

Journal of immunology research

Werner U,Künstner A,Drenckhan M,Pries R,Bruchhage KL,Busch HS,Bachert C,Wollenberg B

Fri Jun 04 00:00:00 EDT 2021

RNA sequencing screening of differentially expressed genes after spinal cord injury.

Neural regeneration research

Li Y,Chen Y,Li X,Wu J,Pan JY,Cai RX,Yang RY,Wang XD

Sun Sep 01 00:00:00 EDT 2019

RNA sequencing screening of differentially expressed genes after spinal cord injury.

Neural regeneration research

Li Y,Chen Y,Li X,Wu J,Pan JY,Cai RX,Yang RY,Wang XD

Sun Sep 01 00:00:00 EDT 2019

N-Acetylcysteine Attenuates Cisplatin-Induced Acute Kidney Injury by Inhibiting the C5a Receptor.

BioMed research international

Huang S,You J,Wang K,Li Y,Zhang Y,Wei H,Liang X,Liu Y

Tue Nov 12 00:00:00 EST 2019

N-Acetylcysteine Attenuates Cisplatin-Induced Acute Kidney Injury by Inhibiting the C5a Receptor.

BioMed research international

Huang S,You J,Wang K,Li Y,Zhang Y,Wei H,Liang X,Liu Y

Tue Nov 12 00:00:00 EST 2019

N-Acetylcysteine Attenuates Cisplatin-Induced Acute Kidney Injury by Inhibiting the C5a Receptor.

BioMed research international

Huang S,You J,Wang K,Li Y,Zhang Y,Wei H,Liang X,Liu Y

Tue Nov 12 00:00:00 EST 2019

Lung injury after asphyxia and hemorrhagic shock in newborn piglets: Analysis of structural and inflammatory changes.

PloS one

Weber B,Mendler MR,Lackner I,von Zelewski A,Höfler S,Baur M,Braun CK,Hummler H,Schwarz S,Pressmar J,Kalbitz M

Fri Feb 21 00:00:00 EST 2020

Time-dependent C5a and C5aR expression in dental pulp cells following stimulation with LTA and LPS.

International journal of molecular medicine

Liu M,Mu H,Peng W,Zhao L,Hu W,Jiang Z,Gao L,Cao X,Li N,Han J

Sun Sep 01 00:00:00 EDT 2019

The hepatocyte-specifically expressed lnc-HSER alleviates hepatic fibrosis by inhibiting hepatocyte apoptosis and epithelial-mesenchymal transition.

Theranostics

Zhang K,Zhang M,Yao Q,Han X,Zhao Y,Zheng L,Li G,Liu Q,Chang Y,Zhang P,Cui H,Shi Z,Chen T,Yao Z,Han T,Hong W

Thu Sep 10 00:00:00 EDT 2020

Diet-Induced Obesity Affects Muscle Regeneration After Murine Blunt Muscle Trauma-A Broad Spectrum Analysis.

Frontiers in physiology

Xu P,Werner JU,Milerski S,Hamp CM,Kuzenko T,Jähnert M,Gottmann P,de Roy L,Warnecke D,Abaei A,Palmer A,Huber-Lang M,Dürselen L,Rasche V,Schürmann A,Wabitsch M,Knippschild U

Thu Oct 01 00:00:00 EDT 2020

Diet-Induced Obesity Affects Muscle Regeneration After Murine Blunt Muscle Trauma-A Broad Spectrum Analysis.

Frontiers in physiology

Xu P,Werner JU,Milerski S,Hamp CM,Kuzenko T,Jähnert M,Gottmann P,de Roy L,Warnecke D,Abaei A,Palmer A,Huber-Lang M,Dürselen L,Rasche V,Schürmann A,Wabitsch M,Knippschild U

Thu Oct 01 00:00:00 EDT 2020

Experimental blunt chest trauma-induced myocardial inflammation and alteration of gap-junction protein connexin 43.

PloS one

Kalbitz M,Amann EM,Bosch B,Palmer A,Schultze A,Pressmar J,Weber B,Wepler M,Gebhard F,Schrezenmeier H,Brenner R,Huber-Lang M

Mon Nov 27 00:00:00 EST 2017

Experimental blunt chest trauma-induced myocardial inflammation and alteration of gap-junction protein connexin 43.

PloS one

Kalbitz M,Amann EM,Bosch B,Palmer A,Schultze A,Pressmar J,Weber B,Wepler M,Gebhard F,Schrezenmeier H,Brenner R,Huber-Lang M

Mon Nov 27 00:00:00 EST 2017

Cardiac Depression in Pigs after Multiple Trauma - Characterization of Posttraumatic Structural and Functional Alterations.

Scientific reports

Kalbitz M,Schwarz S,Weber B,Bosch B,Pressmar J,Hoenes FM,Braun CK,Horst K,Simon TP,Pfeifer R,Störmann P,Hummler H,Gebhard F,Pape HC,Huber-Lang M,Hildebrand F

Tue Dec 19 00:00:00 EST 2017

Cardiac Depression in Pigs after Multiple Trauma - Characterization of Posttraumatic Structural and Functional Alterations.

Scientific reports

Kalbitz M,Schwarz S,Weber B,Bosch B,Pressmar J,Hoenes FM,Braun CK,Horst K,Simon TP,Pfeifer R,Störmann P,Hummler H,Gebhard F,Pape HC,Huber-Lang M,Hildebrand F

Tue Dec 19 00:00:00 EST 2017

Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth.

Scientific reports

Chmilewsky F,Ayaz W,Appiah J,About I,Chung SH

Fri Aug 19 00:00:00 EDT 2016

Nerve Growth Factor Secretion From Pulp Fibroblasts is Modulated by Complement C5a Receptor and Implied in Neurite Outgrowth.

Scientific reports

Chmilewsky F,Ayaz W,Appiah J,About I,Chung SH

Fri Aug 19 00:00:00 EDT 2016

Complement is a central mediator of radiotherapy-induced tumor-specific immunity and clinical response.

Immunity

Surace L,Lysenko V,Fontana AO,Cecconi V,Janssen H,Bicvic A,Okoniewski M,Pruschy M,Dummer R,Neefjes J,Knuth A,Gupta A,van den Broek M

Tue Apr 21 00:00:00 EDT 2015

Complement is a central mediator of radiotherapy-induced tumor-specific immunity and clinical response.

Immunity

Surace L,Lysenko V,Fontana AO,Cecconi V,Janssen H,Bicvic A,Okoniewski M,Pruschy M,Dummer R,Neefjes J,Knuth A,Gupta A,van den Broek M

Tue Apr 21 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin receptor; C5a ligand; C5a Receptor; C5a-R; C5aR; CD88; CD88 antigen; complement C5a receptor; complement component 5 receptor 1; complement component 5, receptor 1

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Gene Aliases: C5A; C5AR; C5AR1; C5R1; CD88; D7Msu1

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UniProt ID: (Human) P21730, (Mouse) P30993, (Rat) P97520

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Entrez Gene ID: (Human) 728, (Mouse) 12273, (Rat) 113959

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Function(s)

complement component C5a binding complement component C5a receptor activity C5a anaphylatoxin receptor activity signal transducer activity G-protein coupled receptor activity anaphylatoxin receptor activity G-protein coupled receptor

Process(es)

activation of MAPK activity apoptotic process chemotaxis inflammatory response immune response cellular defense response signal transduction activation of phospholipase C activity positive regulation of cytosolic calcium ion concentration sensory perception of chemical stimulus positive regulation of vascular endothelial growth factor production positive regulation of macrophage chemotaxis cell proliferation in hindbrain regulation of complement activation neutrophil chemotaxis organ regeneration response to peptidoglycan response to lipopolysaccharide complement component C5a signaling pathway mRNA transcription from RNA polymerase II promoter negative regulation of neuron apoptotic process positive regulation of angiogenesis positive regulation of epithelial cell proliferation defense response to Gram-positive bacterium positive regulation of ERK1 and ERK2 cascade positive regulation of neutrophil chemotaxis complement receptor mediated signaling pathway G-protein coupled receptor signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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C5aR Polyclonal Antibody

C5aR Antibody (21316-1-AP) in ICC/IF

Product Details

21316-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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