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C9orf72 Polyclonal Antibody

C9orf72 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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C9orf72 Antibody (25757-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 25757-1-AP ( C9orf72 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

C9orf72 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

25757-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:300-1:1500

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

C9orf72 Fusion Protein Ag22723 (1-221 aa encoded by BC068445)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody detects the N-terminal of C9orf72.

Immunogen sequence: MSTLCPPPS PAVAKTEIAL SGKSPLLAAT FAYWDNILGP RVRHIWAPKT EQVLLSDGEI TFLANHTLNG EILRNAESGA IDVKFFVLSE KGVIIVSLIF DGNWNGDRST YGLSIILPQT ELSFYLPLHR VCVDRLTHII RKGRIWMHKE RQENVQKIIL EGTERMEDQG QSIIPMLTGE VIPVMELLSS MKSHSVPEEI DIADTVLNDD DIGDSCHEGF LL (1-221 aa encoded by BC068445)

Target Information

C9orf72 may play a role in endosomal trafficking and autophagy. It is known to interact with HNRNPA1, HNRNPA2B1, UBQLN2, and small Rab GTPSases. C9orf72 localizes in the nucleus, cytoplasm, endosome, lysosome, and autophagosome. Mutations in this gene can result in frontotemporal dementia and/or amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD.

Acta neuropathologica

Bauer CS,Cohen RN,Sironi F,Livesey MR,Gillingwater TH,Highley JR,Fillingham DJ,Coldicott I,Smith EF,Gibson YB,Webster CP,Grierson AJ,Bendotti C,De Vos KJ

Thu Sep 01 00:00:00 EDT 2022

Loss of TMEM106B exacerbates C9ALS/FTD DPR pathology by disrupting autophagosome maturation.

Frontiers in cellular neuroscience

Bauer CS,Webster CP,Shaw AC,Kok JR,Castelli LM,Lin YH,Smith EF,Illanes-Álvarez F,Higginbottom A,Shaw PJ,Azzouz M,Ferraiuolo L,Hautbergue GM,Grierson AJ,De Vos KJ

Wed Aug 16 00:00:00 EDT 2023

C9orf72 regulates the unfolded protein response and stress granule formation by interacting with eIF2α.

Theranostics

Zheng W,Wang K,Wu Y,Yan G,Zhang C,Li Z,Wang L,Chen S

Thu Dec 08 00:00:00 EST 2022

Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.

eLife

Laflamme C,McKeever PM,Kumar R,Schwartz J,Kolahdouzan M,Chen CX,You Z,Benaliouad F,Gileadi O,McBride HM,Durcan TM,Edwards AM,Healy LM,Robertson J,McPherson PS

Tue Oct 15 00:00:00 EDT 2019

Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.

eLife

Laflamme C,McKeever PM,Kumar R,Schwartz J,Kolahdouzan M,Chen CX,You Z,Benaliouad F,Gileadi O,McBride HM,Durcan TM,Edwards AM,Healy LM,Robertson J,McPherson PS

Tue Oct 15 00:00:00 EDT 2019

Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.

Nature medicine

Shi Y,Lin S,Staats KA,Li Y,Chang WH,Hung ST,Hendricks E,Linares GR,Wang Y,Son EY,Wen X,Kisler K,Wilkinson B,Menendez L,Sugawara T,Woolwine P,Huang M,Cowan MJ,Ge B,Koutsodendris N,Sandor KP,Komberg J,Vangoor VR,Senthilkumar K,Hennes V,Seah C,Nelson AR,Cheng TY,Lee SJ,August PR,Chen JA,Wisniewski N,Hanson-Smith V,Belgard TG,Zhang A,Coba M,Grunseich C,Ward ME,van den Berg LH,Pasterkamp RJ,Trotti D,Zlokovic BV,Ichida JK

Thu Mar 01 00:00:00 EST 2018

Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.

Nature medicine

Thu Mar 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Guanine nucleotide exchange factor C9orf72; Guanine nucleotide exchange factor C9orf72 homolog; protein C9orf72 homolog

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Gene Aliases: AI840585; ALSFTD; C9orf72; DENND9; DENNL72; FTDALS; FTDALS1

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UniProt ID: (Mouse) Q6DFW0, (Human) Q96LT7

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Entrez Gene ID: (Mouse) 73205, (Human) 203228

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Function(s)

protein binding Rab GTPase binding

Process(es)

endocytosis autophagy

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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C9orf72 Polyclonal Antibody

C9orf72 Antibody (25757-1-AP) in IHC (P)

Product Details

25757-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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