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CALCOCO2 Antibody (PA5-100130) in ICC/IF
Immunofluorescent analysis of CALCOCO2 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CALCOCO2 polyclonal antibody (Product # PA5-100130) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100130
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CALCOCO2(Accession Q13137), corresponding to amino acid residues S343-S393.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815660
Product Specific Information
Antibody detects endogenous levels of total CALCOCO2.
Target Information
The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Antigen nuclear dot 52 kDa protein; calcium binding and coiled-coil domain 2; Calcium-binding and coiled-coil domain-containing protein 2; Nuclear domain 10 protein 52; nuclear domain 10 protein 52 like 1; Nuclear domain 10 protein NDP52; Nuclear dot protein 52
Gene Aliases: 2410154J16Rik; C77254; CALCOCO2; NDP52; Ndp52l1; RGD1559974
UniProt ID: (Human) Q13137, (Mouse) A2A6M5
Entrez Gene ID: (Human) 10241, (Mouse) 76815, (Rat) 303479
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