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CAR Antibody (MA5-29208) in ICC/IF
Immunofluorescence staining of Human CAR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with CAR Recombinant Rabbit Monoclonal Antibody (271) (Product # MA5-29208, 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.
Product Details
MA5-29208
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
271
Immunogen
Recombinant Human CXADR/CAR protein (Met1-Gly237)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785119
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human CXADR/CAR.
Target Information
The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 46 kD coxsackievirus and adenovirus receptor (CAR) protein; CAR; Coxsackievirus and adenovirus receptor; Coxsackievirus B-adenovirus receptor; CVB3-binding protein; HCVADR
Gene Aliases: CAR; CAR4/6; CXADR; HCAR
UniProt ID: (Human) P78310
Entrez Gene ID: (Human) 1525
mitochondrion organization
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
heart development
germ cell migration
epithelial structure maintenance
neutrophil chemotaxis
actin cytoskeleton reorganization
cell-cell junction organization
gamma-delta T cell activation
viral entry into host cell
cardiac muscle fiber development
regulation of immune response
leukocyte migration
transepithelial transport
AV node cell to bundle of His cell communication
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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