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Invitrogen
CASPASE 1 P10 (CLEAVED ALA317) Polyclonal Antibody
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CASPASE 1 P10 (CLEAVED ALA317) Antibody (PA5-105049) in ICC/IF
Immunofluorescent analysis of Caspase 1 p10 (Cleaved Arg317) in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and Caspase 1 p10 (Cleaved Arg317) polyclonal antibody (Product # PA5-105049) using a dilution of 1:100 (primary, 1 hr, 37°C), 1:200 (tubulin), and followed by goat anti-mous... View More
Product Details
PA5-105049
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CASP1(Accession P29466), corresponding to amino acid residues T310-S332.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2816522
Product Specific Information
Antibody detects endogenous levels of fragment of activated Caspase-1 resulting from cleavage adjacent to Ala317.
Target Information
Caspase-1 (CASP1) is a thiol protease that cleaves IL-1 beta between Asp and Ala. This cleavage causes the release of the mature cytokine which is involved in a variety of inflammatory processes. CASP1 is important for defense against pathogen. It cleaves and activates sterol regulatory element binding proteins (SREBPs). CASP1 also promotes apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, CASP1 controls antiviral immunity through the cleavage of CGAS and renders it inactive.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CASP-1; CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); Caspase-1; ICE; IL-1 beta-converting enzyme; IL-1B converting enzyme; IL-1BC; IL1B-convertase; interleukin 1 beta-converting enzyme; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin 1beta converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45
Gene Aliases: CASP1; ICE; IL1BC; IL1BCE; P45
UniProt ID: (Human) P29466, (Mouse) P29452
Entrez Gene ID: (Human) 834, (Mouse) 12362, (Rat) 25166
endopeptidase activity
cysteine-type endopeptidase activity
protein binding
cysteine-type endopeptidase activator activity involved in apoptotic process
cysteine-type endopeptidase activity involved in apoptotic process
peptidase activity
cysteine-type peptidase activity
hydrolase activity
scaffold protein binding
protease
response to hypoxia
proteolysis
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
signal transduction
regulation of autophagy
protein processing
response to lipopolysaccharide
interleukin-1 beta production
response to ATP
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of interleukin-1 alpha secretion
positive regulation of interleukin-1 beta secretion
regulation of inflammatory response
mitochondrial depolarization
membrane hyperpolarization
pyroptosis
cellular response to mechanical stimulus
cellular response to organic substance
programmed necrotic cell death
toxin transport
microglial cell activation
myoblast fusion
memory
response to bacterium
response to organic cyclic compound
response to drug
positive regulation of apoptotic process
positive regulation of circadian sleep/wake cycle, non-REM sleep
positive regulation of cytokine secretion
midgut development
response to toxic substance
lung development
execution phase of apoptosis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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