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CBS Antibody (MA5-49144) in WB
Western blot analysis of CBS using CBS antibody (Product # MA5-49144). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Hela whole cell lysates; Lane 2: human 293T whole cell lysates; Lane 3: human K562 whole cell lysates; Lane 4: rat liver tissue lysates; Lane 5: rat pancreas tissue lysates; Lane 6: mouse liver tissue lysates; Lane 7: mouse pancreas tissue lysates. After elec... View More
Product Details
MA5-49144
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5B5D7
Immunogen
E.coli-derived human CBS recombinant protein (Position: K102-E342).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074802
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Target Information
Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-thionase; Cystathionine beta-synthase; cystathionine-beta-synthase; Hemoprotein H-450; methylcysteine synthase; Serine sulfhydrase
Gene Aliases: AI047524; AI303044; CBS; HIP4
UniProt ID: (Human) P35520, (Rat) P32232, (Mouse) Q91WT9
Entrez Gene ID: (Human) 875, (Rat) 24250, (Mouse) 12411
cystathionine beta-synthase activity
cysteine synthase activity
protein binding
oxygen binding
enzyme binding
heme binding
pyridoxal phosphate binding
ubiquitin protein ligase binding
identical protein binding
protein homodimerization activity
metal ion binding
nitrite reductase (NO-forming) activity
carbon monoxide binding
nitric oxide binding
modified amino acid binding
S-adenosyl-L-methionine binding
lyase activity
lyase
metabolite interconversion enzyme
cysteine biosynthetic process from serine
L-serine metabolic process
L-serine catabolic process
cellular amino acid biosynthetic process
cysteine biosynthetic process via cystathionine
cysteine biosynthetic process
transsulfuration
L-cysteine catabolic process
DNA protection
homocysteine catabolic process
homocysteine metabolic process
oxidation-reduction process
hydrogen sulfide biosynthetic process
endochondral ossification
blood vessel remodeling
superoxide metabolic process
cerebellum morphogenesis
regulation of cGMP metabolic process
response to nutrient levels
negative regulation of apoptotic process
regulation of JUN kinase activity
regulation of blood vessel size
response to folic acid
maternal process involved in female pregnancy
cartilage development involved in endochondral bone morphogenesis
cellular response to hypoxia
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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