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Invitrogen
CCL1 Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
CCL1 Antibody (PA5-47952) in Neu
Neutralization of CCL1 in BaF3 mouse pro‚B cell line transfected with mouse CCR8. Samples were incubated in CCL1 polyclonal antibody (Product # PA5-47952). Recombinant Mouse CCL1/TCA‚3 chemoattracts the BaF3 mouse pro‚B cell line transfected with mouse CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL1/TCA‚3 (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL... View More
Product Details
PA5-47952
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Sf 21-derived recombinant mouse CCL1/I-309/TCA-3 Lys24-Cys92
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609540
Product Specific Information
In Western blots, approximately 10% cross-reactivity with recombinant humanI-309 is observed and 2% cross-reactivity with recombinant mouse MCP-5 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 1; CCL 1; CCR8 ligand; P500; SIS-epsilon; small inducible cytokine A1; Small-inducible cytokine A1; T-cell activation protein 3; TCA-3; TCA3
Gene Aliases: BF534335; Ccl1; I-309; Scya1; Tca-3; Tca3
UniProt ID: (Mouse) P10146
Entrez Gene ID: (Mouse) 20290
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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