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Invitrogen
CCL2 (MCP-1) Monoclonal Antibody (4E2), eBioscience™
Product Details
14-7091-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
4E2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_468428
Product Specific Information
Description: The 4E2/MCP antibody reacts with mouse monocyte chemoattractant protein-1 (MCP-1).
Applications Reported: This 4E2 antibody has been reported for use in ELISA.
Applications Tested: The 4E2 antibody has been tested as the capture antibody in a sandwich ELISA in combination with the Biotin 2H5 (Product # 13-7096-85) antibody for detection of mouse CCL2 (MCP-1, MCP1, MCAF). A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 2; H-MCP-1; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic protein 1; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; Small-inducible cytokine A2
Gene Aliases: AI323594; Ccl2; HC11; JE; MCAF; MCP-1; MCP1; Scya2; Sigje; SMC-CF
UniProt ID: (Mouse) P10148
Entrez Gene ID: (Mouse) 20296
angiogenesis
positive regulation of leukocyte mediated cytotoxicity
positive regulation of endothelial cell proliferation
leukocyte migration involved in inflammatory response
positive regulation of leukocyte migration
positive regulation of cellular extravasation
cellular calcium ion homeostasis
chemotaxis
inflammatory response
immune response
cytoskeleton organization
transforming growth factor beta receptor signaling pathway
glial cell migration
regulation of cell shape
response to heat
response to wounding
regulation of vascular endothelial growth factor production
positive regulation of macrophage chemotaxis
negative regulation of angiogenesis
cytokine-mediated signaling pathway
positive regulation of cell-cell adhesion
neutrophil chemotaxis
positive regulation of tumor necrosis factor production
positive regulation of collagen biosynthetic process
helper T cell extravasation
monocyte homeostasis
positive regulation of cell adhesion
vascular endothelial growth factor receptor signaling pathway
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of synaptic transmission
positive regulation of T cell activation
positive regulation of nitric-oxide synthase biosynthetic process
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
positive regulation of monocyte chemotaxis
positive regulation of immune complex clearance by monocytes and macrophages
positive regulation of protein targeting to membrane
positive regulation of apoptotic cell clearance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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