Invitrogen

CCL2 (MCP-1) Monoclonal Antibody (5D3-F7), eBioscience™

Product Details

14-7099-81

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

View 1 publication 1 publication

ELISA (ELISA)

1-4 µg/mL

View 8 publications 8 publications

Immunoprecipitation (IP)

Assay-Dependent

Functional Assay (Functional)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

5D3-F7

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468432

Product Specific Information

Description: The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.

Applications Reported: The 5D3-F7 antibody has been reported to be useful for detection of MCP-1 in a sandwich ELISA, neutralization of MCP-1 bioactivity, intracellular staining for flow cytometric analysis, IP, and immunohistochemical staining of frozen tissue sections.

Applications Tested: The affinity purified 5D3-F7 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the biotinylated 2H5 (13-7096) antibody for detection and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

The Functional Grade purified 5D3-F7 antibody has been tested for neutralization of MCP-1 bioactivity.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Peripheral immunophenotyping of AITD subjects reveals alterations in immune cells in pediatric vs adult-onset AITD.

iScience

Stensland ZC,Coleman BM,Rihanek M,Baxter RM,Gottlieb PA,Hsieh EWY,Sarapura VD,Simmons KM,Cambier JC,Smith MJ

14-7099-81 was used in Miscellaneous to demonstrate diversity of AITD by showing differences in immune cell phenotypes and function based on age of onset.

Fri Jan 21 00:00:00 EST 2022

Gestational diabetes impacts fetal precursor cell responses with potential consequences for offspring.

Stem cells translational medicine

Algaba-Chueca F,Maymó-Masip E,Ejarque M,Ballesteros M,Llauradó G,López C,Guarque A,Serena C,Martínez-Guasch L,Gutiérrez C,Bosch R,Vendrell J,Megía A,Fernández-Veledo S

14-7099-81 was used in Functional assay to suggest that the GDM environment could program stem cells and subsequently favor metabolic dysfunction later in life.

Sun Mar 01 00:00:00 EST 2020

Aggregation and Its Influence on the Immunomodulatory Activity of Synthetic Innate Defense Regulator Peptides.

Cell chemical biology

Haney EF,Wu BC,Lee K,Hilchie AL,Hancock REW

14-7099-81 was used in ELISA to examine the effect of aggregation on the immunomodulatory activity of a synthetic innate defense regulator peptide, 1018 (VRLIVAVRIWRR-NH2).

Thu Aug 17 00:00:00 EDT 2017

Protective Role for B-1b B Cells and IgM in Obesity-Associated Inflammation, Glucose Intolerance, and Insulin Resistance.

Arteriosclerosis, thrombosis, and vascular biology

Harmon DB,Srikakulapu P,Kaplan JL,Oldham SN,McSkimming C,Garmey JC,Perry HM,Kirby JL,Prohaska TA,Gonen A,Hallowell P,Schirmer B,Tsimikas S,Taylor AM,Witztum JL,McNamara CA

14-7099 was used in Flow cytometry/Cell sorting to investigate the role of B-cell functions in obesity-induced metabolic dysfunction.

Fri Apr 01 00:00:00 EDT 2016

Development and validation of an immunosensor for monocyte chemotactic protein 1 using a silicon photonic microring resonator biosensing platform.

Clinical biochemistry

Valera E,Shia WW,Bailey RC

14-7099 was used in an ELISA assay to develop a silicon photonic immunosensor for the detection of clinically-relevant concentrations of monocyte chemotactic protein 1.

Fri Jan 01 00:00:00 EST 2016

Mucosal Administration of Collagen V Ameliorates the Atherosclerotic Plaque Burden by Inducing Interleukin 35-dependent Tolerance.

The Journal of biological chemistry

Park AC,Huang G,Jankowska-Gan E,Massoudi D,Kernien JF,Vignali DA,Sullivan JA,Wilkes DS,Burlingham WJ,Greenspan DS

14-7099 was used in Western Blotting to determine whether induction of immune tolerance to col(V) might ameliorate atherosclerosis, providing further evidence for a causal role for col(V) autoimmunity in atherogenesis.

Fri Feb 12 00:00:00 EST 2016

Mechanism and role of MCP-1 upregulation upon chikungunya virus infection in human peripheral blood mononuclear cells.

Scientific reports

Ruiz Silva M,van der Ende-Metselaar H,Mulder HL,Smit JM,Rodenhuis-Zybert IA

14-7099 was used in Neutralization experiments to investigate the role and mechanism of MCP-1 production upon chikungunya virus infection of human peripheral blood mononuclear cells.

Thu Aug 25 00:00:00 EDT 2016

Demyelination causes adult CNS progenitors to revert to an immature state and express immune cues that support their migration.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Moyon S,Dubessy AL,Aigrot MS,Trotter M,Huang JK,Dauphinot L,Potier MC,Kerninon C,Melik Parsadaniantz S,Franklin RJ,Lubetzki C

14-7099 was used in Immunohistochemistry on frozen tissues to invesitgate how oligodendrocyte progenitor cells become activated after demyelination and how this enables them to contribute to remyelination.

Wed Jan 07 00:00:00 EST 2015

Magnetically-actuated, bead-enhanced silicon photonic immunosensor.

Analytical methods : advancing methods and applications

Valera E,McClellan MS,Bailey RC

14-7099-81 was used in Functional assays to demonstrate that addition of magnetic actuation into bead-enhanced sandwich assays on a silicon photonic biosensor platform might improve biomarker detection.

Wed Oct 21 00:00:00 EDT 2015

Oxidized low-density lipoprotein induces long-term proinflammatory cytokine production and foam cell formation via epigenetic reprogramming of monocytes.

Arteriosclerosis, thrombosis, and vascular biology

Bekkering S,Quintin J,Joosten LA,van der Meer JW,Netea MG,Riksen NP

14-7099 was used in an ELISA assay to study the role of oxidised low-density lipoprotein (oxLDL) in inducing a long lasting proinflammatory phenotype.

Fri Aug 01 00:00:00 EDT 2014

Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus.

The Journal of clinical investigation

Price JV,Haddon DJ,Kemmer D,Delepine G,Mandelbaum G,Jarrell JA,Gupta R,Balboni I,Chakravarty EF,Sokolove J,Shum AK,Anderson MS,Cheng MH,Robinson WH,Browne SK,Holland SM,Baechler EC,Utz PJ

14-7099 was used in an ELISA assay to study how serum factor protein microarrays facilitate the detection of auto-antibody reactivity to serum factors in human samples.

Sun Dec 01 00:00:00 EST 2013

Ceramide 1-phosphate induces macrophage chemoattractant protein-1 release: involvement in ceramide 1-phosphate-stimulated cell migration.

American journal of physiology. Endocrinology and metabolism

Arana L,Ordoñez M,Ouro A,Rivera IG,Gangoiti P,Trueba M,Gomez-Muñoz A

14-7099 was used in Functional assays to study the molecular mechanisms underlying the role of ceramide 1-phosphate in inflammatory responses and cell migration.

Sat Jun 01 00:00:00 EDT 2013

The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells.

Journal of leukocyte biology

Nijnik A,Pistolic J,Cho P,Filewod NC,Falsafi R,Ramin A,Harder KW,Hancock RE

14-7099 was used in an ELISA assay to elucidate the common molecular mechanisms mediating the chemokine induction activity of natural and synthetic cationic peptides in monocytic cells.

Sun Apr 01 00:00:00 EDT 2012

The Pseudomonas aeruginosa PhoP-PhoQ two-component regulatory system is induced upon interaction with epithelial cells and controls cytotoxicity and inflammation.

Infection and immunity

Gellatly SL,Needham B,Madera L,Trent MS,Hancock RE

14-7099 was used in an ELISA assay to assess how disruption of the regulatory system, PhoPQ, in gram-negative species of bacteria affects virulence.

Sat Sep 01 00:00:00 EDT 2012

Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment.

Journal of immunology (Baltimore, Md. : 1950)

Nijnik A,Madera L,Ma S,Waldbrook M,Elliott MR,Easton DM,Mayer ML,Mullaly SC,Kindrachuk J,Jenssen H,Hancock RE

14-7099 was used in an ELISA assay to study the importance of the chemokine induction activity of host defence peptides in protection against bacterial infections.

Mon Mar 01 00:00:00 EST 2010

Tumor cell-microenvironment interaction models coupled with clinical validation reveal CCL2 and SNCG as two predictors of colorectal cancer hepatic metastasis.

Clinical cancer research : an official journal of the American Association for Cancer Research

Hu H,Sun L,Guo C,Liu Q,Zhou Z,Peng L,Pan J,Yu L,Lou J,Yang Z,Zhao P,Ran Y

14-7099 was used in Immunohistochemistry to investigate novel biological markers to enable the prediction of colorectal cancer liver metastasis.

Tue Sep 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; RP23-350G1.3; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; Small-inducible cytokine A2

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Gene Aliases: CCL2; GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF

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UniProt ID: (Human) P13500

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Entrez Gene ID: (Human) 6347

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Function(s)

protein kinase activity receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding cytokine intercellular signal molecule

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CCL2 (MCP-1) Monoclonal Antibody (5D3-F7), eBioscience™

Product Details

14-7099-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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