Invitrogen

CCL21 Polyclonal Antibody, Biotin, PeproTech®

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View all (22) CCL21 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

500-P109BT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
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Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Exodus-2 (CCL21)
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929238

Product Specific Information

AA Sequence of recombinant protein: discontinued.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Exodus-2 (CCL21). Anti-Human Exodus-2 (CCL21)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human Exodus-2 (CCL21) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Exodus-2 (CCL21) (500-P109) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Exodus-2 (CCL21).

Western Blot: To detect hExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P109BT-1MG will be provided as 2 x 500 µg

Target Information

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 6 Ckine; 6Ckine; beta chemokine exodus-2; Beta-chemokine exodus-2; C-C motif chemokine 21; chemokine (C-C motif) ligand 21; Efficient Chemoattractant for Lymphocytes; secondary lymphoid tissue chemokine; Secondary lymphoid-tissue chemokine; SLC; small inducible cytokine subfamily A (Cys-Cys), member 21; Small-inducible cytokine A21

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Gene Aliases: 6Ckine; CCL21; CKb9; ECL; SCYA21; SLC; TCA4; UNQ784/PRO1600

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UniProt ID: (Human) O00585

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Entrez Gene ID: (Human) 6366

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Function(s)
chemokine activity CCR7 chemokine receptor binding chemokine receptor binding CCR chemokine receptor binding cytokine intercellular signal molecule
Process(es)
establishment of T cell polarity immunological synapse formation positive regulation of cell-matrix adhesion dendritic cell chemotaxis monocyte chemotaxis positive regulation of dendritic cell antigen processing and presentation inflammatory response immune response G-protein coupled receptor signaling pathway cell-cell signaling positive regulation of glycoprotein biosynthetic process positive regulation of actin filament polymerization positive regulation of pseudopodium assembly T cell costimulation ruffle organization positive regulation of cell adhesion mediated by integrin response to prostaglandin E mesangial cell-matrix adhesion positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of phosphatidylinositol 3-kinase activity positive regulation of protein kinase activity positive regulation of JNK cascade lymphocyte chemotaxis positive regulation of receptor-mediated endocytosis cell maturation positive regulation of chemotaxis release of sequestered calcium ion into cytosol positive regulation of filopodium assembly positive regulation of protein kinase B signaling cell chemotaxis chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor positive regulation of neutrophil chemotaxis activation of GTPase activity dendritic cell dendrite assembly negative regulation of leukocyte tethering or rolling negative regulation of leukocyte apoptotic process positive regulation of cell motility positive regulation of T cell migration positive regulation of myeloid dendritic cell chemotaxis negative regulation of dendritic cell dendrite assembly
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