Invitrogen

CCL21 Polyclonal Antibody, PeproTech®

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CCL21 Antibody (500-P114-1MG) in ELISA

Sandwich ELISA: To detect Murine Exodus-2 (CCL21) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL21 Polyclonal Antibody (Product # 500-P114-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL21 Polyclonal Antibody, Biotin (Product # 500-P114BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine Exodus-2 (CCL21).

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Product Details

500-P114-1MG

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

In vitro Assay (IV)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Immunostaining (IS)

View 6 publications 6 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine Exodus-2 (CCL21)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929276

Product Specific Information

AA Sequence of recombinant protein: SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine Exodus-2 (CCL21). Anti-Murine Exodus-2 (CCL21)-specific antibody was purified by affinity chromatography employing an immobilized Murine Exodus-2 (CCL21) matrix.

Sandwich ELISA: To detect Murine Exodus-2 (CCL21) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine Exodus-2 (CCL21) (500-P114Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine Exodus-2 (CCL21).

Western Blot: To detect Murine Exodus-2 (CCL21) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine Exodus-2 (CCL21) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines the protein encoded by this gene inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nociception-Dependent CCL21 Induces Dorsal Root Ganglia Axonal Growth via CCR7-ERK Activation.

Frontiers in immunology

Mesquida-Veny F,Martínez-Torres S,Del Rio JA,Hervera A

500-P114 was used in Immunohistochemistry to investigate proteins and chemokines in cell migration.

Tue Aug 02 00:00:00 EDT 2022

Scavenging of soluble and immobilized CCL21 by ACKR4 regulates peripheral dendritic cell emigration.

Proceedings of the National Academy of Sciences of the United States of America

Bastow CR,Bunting MD,Kara EE,McKenzie DR,Caon A,Devi S,Tolley L,Mueller SN,Frazer IH,Harvey N,Condina MR,Young C,Hoffmann P,McColl SR,Comerford I

Tue Apr 27 00:00:00 EDT 2021

Low-dose paclitaxel improves the therapeutic efficacy of recombinant adenovirus encoding CCL21 chemokine against murine cancer.

Cancer science

Chen P,Luo S,Wen YJ,Li YH,Li J,Wang YS,Du LC,Zhang P,Tang J,Yang DB,Hu HZ,Zhao X,Wei YQ

Sat Nov 01 00:00:00 EDT 2014

Low-dose paclitaxel improves the therapeutic efficacy of recombinant adenovirus encoding CCL21 chemokine against murine cancer.

Cancer science

Chen P,Luo S,Wen YJ,Li YH,Li J,Wang YS,Du LC,Zhang P,Tang J,Yang DB,Hu HZ,Zhao X,Wei YQ

Sat Nov 01 00:00:00 EDT 2014

Low-dose paclitaxel improves the therapeutic efficacy of recombinant adenovirus encoding CCL21 chemokine against murine cancer.

Cancer science

Chen P,Luo S,Wen YJ,Li YH,Li J,Wang YS,Du LC,Zhang P,Tang J,Yang DB,Hu HZ,Zhao X,Wei YQ

Sat Nov 01 00:00:00 EDT 2014

Role of high endothelial venule-expressed heparan sulfate in chemokine presentation and lymphocyte homing.

Journal of immunology (Baltimore, Md. : 1950)

Tsuboi K,Hirakawa J,Seki E,Imai Y,Yamaguchi Y,Fukuda M,Kawashima H

Mon Jul 01 00:00:00 EDT 2013

Lymph node T cell homeostasis relies on steady state homing of dendritic cells.

Immunity

Wendland M,Willenzon S,Kocks J,Davalos-Misslitz AC,Hammerschmidt SI,Schumann K,Kremmer E,Sixt M,Hoffmeyer A,Pabst O,Förster R

Fri Dec 23 00:00:00 EST 2011

Immobilized chemokine fields and soluble chemokine gradients cooperatively shape migration patterns of dendritic cells.

Immunity

Schumann K,Lämmermann T,Bruckner M,Legler DF,Polleux J,Spatz JP,Schuler G,Förster R,Lutz MB,Sorokin L,Sixt M

Fri May 28 00:00:00 EDT 2010

Immobilized chemokine fields and soluble chemokine gradients cooperatively shape migration patterns of dendritic cells.

Immunity

Schumann K,Lämmermann T,Bruckner M,Legler DF,Polleux J,Spatz JP,Schuler G,Förster R,Lutz MB,Sorokin L,Sixt M

Fri May 28 00:00:00 EDT 2010

Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces.

Nature immunology

Woolf E,Grigorova I,Sagiv A,Grabovsky V,Feigelson SW,Shulman Z,Hartmann T,Sixt M,Cyster JG,Alon R

Mon Oct 01 00:00:00 EDT 2007

Local expression of secondary lymphoid tissue chemokine delivered by adeno-associated virus within the tumor bed stimulates strong anti-liver tumor immunity.

Journal of virology

Liang CM,Zhong CP,Sun RX,Liu BB,Huang C,Qin J,Zhou S,Shan J,Liu YK,Ye SL

Sat Sep 01 00:00:00 EDT 2007

Binding of lymphoid chemokines to collagen IV that accumulates in the basal lamina of high endothelial venules: its implications in lymphocyte trafficking.

Journal of immunology (Baltimore, Md. : 1950)

Yang BG,Tanaka T,Jang MH,Bai Z,Hayasaka H,Miyasaka M

Mon Oct 01 00:00:00 EDT 2007

Binding of lymphoid chemokines to collagen IV that accumulates in the basal lamina of high endothelial venules: its implications in lymphocyte trafficking.

Journal of immunology (Baltimore, Md. : 1950)

Yang BG,Tanaka T,Jang MH,Bai Z,Hayasaka H,Miyasaka M

Mon Oct 01 00:00:00 EDT 2007

Vesicle-mediated transport and release of CCL21 in endangered neurons: a possible explanation for microglia activation remote from a primary lesion.

The Journal of neuroscience : the official journal of the Society for Neuroscience

de Jong EK,Dijkstra IM,Hensens M,Brouwer N,van Amerongen M,Liem RS,Boddeke HW,Biber K

Wed Aug 17 00:00:00 EDT 2005

Intratumoral administration of dendritic cells overexpressing CCL21 generates systemic antitumor responses and confers tumor immunity.

Clinical cancer research : an official journal of the American Association for Cancer Research

Yang SC,Hillinger S,Riedl K,Zhang L,Zhu L,Huang M,Atianzar K,Kuo BY,Gardner B,Batra RK,Strieter RM,Dubinett SM,Sharma S

Thu Apr 15 00:00:00 EDT 2004

Pivotal role of CXCR3 in melanoma cell metastasis to lymph nodes.

Cancer research

Kawada K,Sonoshita M,Sakashita H,Takabayashi A,Yamaoka Y,Manabe T,Inaba K,Minato N,Oshima M,Taketo MM

Tue Jun 01 00:00:00 EDT 2004

Lymphoid tissue homing chemokines are expressed in chronic inflammation.

The American journal of pathology

Hjelmström P,Fjell J,Nakagawa T,Sacca R,Cuff CA,Ruddle NH

Sat Apr 01 00:00:00 EST 2000

Bioinformatics

Protein Aliases: 6 Ckine; 6Ckine; beta chemokine exodus-2; Beta-chemokine exodus-2; C-C motif chemokine 21a; C-C motif chemokine 21b; C-C motif chemokine 21c; CC chemokine 6Ckine-ser; Ccl21-leu; CCL21-Ser; exodus-2; secondary lymphoid organ chemokine; small inducible cytokine A21; small inducible cytokine A21a; small inducible cytokine A21c; Small-inducible cytokine A21a; Small-inducible cytokine A21b; Small-inducible cytokine A21c; TCA4; Thymus-derived chemotactic agent 4

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Gene Aliases: 6CKBAC1; 6CKBAC2; 6Ckine; AI159701; ALP; AW987545; Ccl21a; Ccl21b; Ccl21c; CKb9; exodus-2; plt; Scya21; SCYA21a; Scya21b; Scya21c; SLC; TCA4

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UniProt ID: (Mouse) P84444, (Mouse) P86792

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Entrez Gene ID: (Mouse) 18829, (Mouse) 100042493, (Mouse) 65956

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Function(s)

chemokine activity chemokine receptor binding cytokine intercellular signal molecule

Process(es)

lymph node development induction of positive chemotaxis cell chemotaxis positive regulation of T cell migration leukocyte chemotaxis negative regulation of myeloid cell differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CCL21 Polyclonal Antibody, PeproTech®

CCL21 Antibody (500-P114-1MG) in ELISA

Product Details

500-P114-1MG

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Neutralization (Neu)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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