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CCL3 Antibody (500-M74-500UG) in WB
Western Blot: To detect Human MIP-1alpha (CCL3) by Western Blot analysis CCL3 Monoclonal Antibody (Product # 500-M74-500UG) can be used at a concentration of 2.0-4.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-1alpha (CCL3) is 1.0-2.0 ng/lane, under non-reducing conditions.
Product Details
500-M74-500UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
Class
Monoclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MIP-1alpha (CCL3)
Conjugate
Form
Lyophilized
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_3074169
Product Specific Information
AA Sequence of recombinant protein: ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA.
Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human MIP-1alpha (CCL3). Anti-Human MIP-1alpha (CCL3)-specific antibody was purified from cell culture by Protein G affinity chromatography.
Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 2000 pg/mL of Recombinant Human MIP-1alpha (CCL3) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human MIP-1alpha (CCL3) (500-P38GBT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/mL.
Western Blot: To detect Human MIP-1alpha (CCL3) by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-1alpha (CCL3) is 1.0-2.0 ng/lane, under non-reducing conditions.
Target Information
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein
Gene Aliases: CCL3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3
UniProt ID: (Human) P10147
Entrez Gene ID: (Human) 6348
protein kinase activity
calcium-dependent protein kinase C activity
protein binding
chemokine activity
phospholipase activator activity
kinase activity
CCR1 chemokine receptor binding
CCR5 chemokine receptor binding
chemoattractant activity
identical protein binding
cytokine
intercellular signal molecule
MAPK cascade
osteoblast differentiation
cell activation
monocyte chemotaxis
calcium ion transport
cellular calcium ion homeostasis
exocytosis
chemotaxis
inflammatory response
cytoskeleton organization
G-protein coupled receptor signaling pathway
cell-cell signaling
behavior
regulation of cell shape
response to toxic substance
positive regulation of gene expression
negative regulation of gene expression
T cell chemotaxis
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
calcium-mediated signaling
signaling
positive regulation of cell migration
negative regulation of bone mineralization
neutrophil chemotaxis
lipopolysaccharide-mediated signaling pathway
positive regulation of tumor necrosis factor production
eosinophil degranulation
protein kinase B signaling
positive regulation of neuron apoptotic process
positive regulation of GTPase activity
astrocyte cell migration
negative regulation by host of viral transcription
negative regulation of osteoclast differentiation
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of interleukin-1 beta secretion
positive regulation of inflammatory response
positive regulation of calcium-mediated signaling
positive chemotaxis
positive regulation of protein kinase B signaling
positive regulation of calcium ion transport
regulation of sensory perception of pain
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
response to cholesterol
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to organic cyclic compound
granulocyte chemotaxis
positive regulation of calcium ion import
positive regulation of natural killer cell chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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