Invitrogen

CCL3 Polyclonal Antibody, PeproTech®

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

500-P38-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.5-2.0 µg/mL
View 2 publications 2 publications

In vitro Assay (IV)

-
View 1 publication 1 publication

Immunostaining (IS)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human MIP-1alpha (CCL3)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930498

Product Specific Information

AA Sequence of recombinant protein: ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-1alpha (CCL3). Anti-Human MIP-1alpha (CCL3)-specific antibody was purified by affinity chromatography employing an immobilized Human MIP-1alpha (CCL3) matrix.

Sandwich ELISA: To detect Human MIP-1alpha (CCL3) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIP-1alpha (CCL3) (500-P38Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-1alpha (CCL3).

Western Blot: To detect Human MIP-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-C motif chemokine 3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein

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Gene Aliases: CCL3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3

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UniProt ID: (Human) P10147

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Entrez Gene ID: (Human) 6348

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Function(s)
protein kinase activity calcium-dependent protein kinase C activity protein binding chemokine activity phospholipase activator activity kinase activity CCR1 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity identical protein binding cytokine intercellular signal molecule
Process(es)
MAPK cascade osteoblast differentiation cell activation monocyte chemotaxis calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response cytoskeleton organization G-protein coupled receptor signaling pathway cell-cell signaling behavior regulation of cell shape response to toxic substance positive regulation of gene expression negative regulation of gene expression T cell chemotaxis release of sequestered calcium ion into cytosol by sarcoplasmic reticulum calcium-mediated signaling signaling positive regulation of cell migration negative regulation of bone mineralization neutrophil chemotaxis lipopolysaccharide-mediated signaling pathway positive regulation of tumor necrosis factor production eosinophil degranulation protein kinase B signaling positive regulation of neuron apoptotic process positive regulation of GTPase activity astrocyte cell migration negative regulation by host of viral transcription negative regulation of osteoclast differentiation eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of interleukin-1 beta secretion positive regulation of inflammatory response positive regulation of calcium-mediated signaling positive chemotaxis positive regulation of protein kinase B signaling positive regulation of calcium ion transport regulation of sensory perception of pain chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade response to cholesterol cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to organic cyclic compound granulocyte chemotaxis positive regulation of calcium ion import positive regulation of natural killer cell chemotaxis
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