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Invitrogen
CCL3 Monoclonal Antibody (93342), FITC
FIGURE: 1 / 1
CCL3 Antibody (MA5-23564) in Flow
Flow cytometry of CCL3 in Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CD14 APC‚conjugated Monoclonal Antibody. Samples were incubated in either (A) CCL3 monoclonal antibody (Product # MA5-23564) or (B) Mouse IgG2B Fluorescein Isotype Control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I.
Product Details
MA5-23564
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2B
Class
Monoclonal
Type
Antibody
Clone
93342
Immunogen
E. coli-derived recombinant human CCL3/MIP-1 alpha Ala27-Ala92
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Purification
Protein A/G
Storage buffer
Buffered saline solution with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609537
Target Information
Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein
Gene Aliases: CCL3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3
UniProt ID: (Human) P10147
Entrez Gene ID: (Human) 6348
protein kinase activity
calcium-dependent protein kinase C activity
protein binding
chemokine activity
phospholipase activator activity
kinase activity
CCR1 chemokine receptor binding
CCR5 chemokine receptor binding
chemoattractant activity
identical protein binding
cytokine
intercellular signal molecule
MAPK cascade
osteoblast differentiation
cell activation
monocyte chemotaxis
calcium ion transport
cellular calcium ion homeostasis
exocytosis
chemotaxis
inflammatory response
cytoskeleton organization
G-protein coupled receptor signaling pathway
cell-cell signaling
behavior
regulation of cell shape
response to toxic substance
positive regulation of gene expression
negative regulation of gene expression
T cell chemotaxis
release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
calcium-mediated signaling
signaling
positive regulation of cell migration
negative regulation of bone mineralization
neutrophil chemotaxis
lipopolysaccharide-mediated signaling pathway
positive regulation of tumor necrosis factor production
eosinophil degranulation
protein kinase B signaling
positive regulation of neuron apoptotic process
positive regulation of GTPase activity
astrocyte cell migration
negative regulation by host of viral transcription
negative regulation of osteoclast differentiation
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of interleukin-1 beta secretion
positive regulation of inflammatory response
positive regulation of calcium-mediated signaling
positive chemotaxis
positive regulation of protein kinase B signaling
positive regulation of calcium ion transport
regulation of sensory perception of pain
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
response to cholesterol
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to organic cyclic compound
granulocyte chemotaxis
positive regulation of calcium ion import
positive regulation of natural killer cell chemotaxis
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