Invitrogen

CCL3 (MIP-1 alpha) Monoclonal Antibody (CR3M), PE, eBioscience™

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

12-9706-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.015 µg)/test
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CR3M

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572722

Product Specific Information

Description: This CR3M monoclonal antibody reacts with human CCL3. CCL3, also known as MIP-1 alpha (Macrophage Inflammatory Protein 1 alpha), is a member of the CC- subfamily of chemokines and is most closely related to CCL4, or MIP-1 beta. These proteins play critical roles in the recruitment of leukocytes to the site of inflammation. While both CCL3 and CCL4 will attract monocytes, macrophages, and dendritic cells, CCL3 preferentially attracts CD8+ T cells, while CD4+ T cells are more responsive to CCL4. In addition to its chemotactic functions, CCL3 induces inflammatory cytokine secretion, mast cell degranulation, and NK cell activation. It has also been reported to inhibit hematopoetic stem cell proliferation and may be responsible for the maintenance of these cells in a quiescent state.

CCL3 signaling is mediated by the G protein-coupled receptors CCR1, CCR4, and CCR5, which are shared with CCL4 and CCL5 (RANTES). CCR5 is the primary co-receptor for HIV entry, which the virus binds through the gp120 envelope protein. All CCR5 ligands demonstrate potent inhibition of virus entry into the cell, both through steric hindrance of gp120-CCR5 interaction, and ligand-mediated receptor internalization.

This CR3M clone is specific to CCL3, with no detectable cross-reactivity to Human CCL4 (MIP-1 beta).

Applications Reported: This CR3M antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This CR3M antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Both MIP-1alpha and MIP-1beta are structurally and functionally related CC chemokines. They participate in host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells (e.g. macrophages, lymphocytes and NK cells). While both MIP-1alpha and MIP-1beta exert similar effects on monocytes, their effect on lymphocytes differ; with MIP-1alpha selectively attracting CD8+ lymphocytes, and MIP-1beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1alpha and MIP-1beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1alpha and MIP-1beta are active on human and murine hematopoietic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-C motif chemokine 3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; H-MIP-1-alpha; LD78-alpha; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein

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Gene Aliases: CCL3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3

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UniProt ID: (Human) P10147

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Entrez Gene ID: (Human) 6348

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Function(s)
protein kinase activity calcium-dependent protein kinase C activity protein binding chemokine activity phospholipase activator activity kinase activity CCR1 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity identical protein binding cytokine intercellular signal molecule
Process(es)
MAPK cascade osteoblast differentiation cell activation monocyte chemotaxis calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response cytoskeleton organization G-protein coupled receptor signaling pathway cell-cell signaling behavior regulation of cell shape response to toxic substance positive regulation of gene expression negative regulation of gene expression T cell chemotaxis release of sequestered calcium ion into cytosol by sarcoplasmic reticulum calcium-mediated signaling signaling positive regulation of cell migration negative regulation of bone mineralization neutrophil chemotaxis lipopolysaccharide-mediated signaling pathway positive regulation of tumor necrosis factor production eosinophil degranulation protein kinase B signaling positive regulation of neuron apoptotic process positive regulation of GTPase activity astrocyte cell migration negative regulation by host of viral transcription negative regulation of osteoclast differentiation eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of interleukin-1 beta secretion positive regulation of inflammatory response positive regulation of calcium-mediated signaling positive chemotaxis positive regulation of protein kinase B signaling positive regulation of calcium ion transport regulation of sensory perception of pain chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade response to cholesterol cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to organic cyclic compound granulocyte chemotaxis positive regulation of calcium ion import positive regulation of natural killer cell chemotaxis
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