Invitrogen

CCL5 (RANTES) Polyclonal Antibody, PeproTech®

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

500-P118-1MG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

ELISA (ELISA)

0.5-2.0 µg/mL
View 4 publications 4 publications

In vitro Assay (IV)

-
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 3 publications 3 publications

Immunostaining (IS)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Mouse

Published species

Mouse, Rat

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Murine RANTES (CCL5)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929301

Product Specific Information

AA Sequence of recombinant protein: SPYGSDTTPC CFAYLSLALP RAHVKEYFYT SSKCSNLAVV FVTRRNRQVC ANPEKKWVQE YINYLEMS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine RANTES (CCL5). Anti-Murine RANTES (CCL5)-specific antibody was purified by affinity chromatography employing an immobilized Murine RANTES (CCL5) matrix.

Sandwich ELISA: To detect Murine RANTES (CCL5) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine RANTES (CCL5) (500-P118Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine RANTES (CCL5).

Western Blot: To detect Murine RANTES (CCL5) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine RANTES (CCL5) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-C motif chemokine 5; MGC17164; MuRantes; OTTHUMP00000197106; SIS δ; SIS-delta; SISδ; small inducible cytokine A5; Small-inducible cytokine A5; T-cell-specific protein RANTES

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Gene Aliases: Ccl5; MuRantes; RANTES; Scya5; SISd; TCP228

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UniProt ID: (Mouse) P30882

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Entrez Gene ID: (Mouse) 20304

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Function(s)
phosphatidylinositol phospholipase C activity protein kinase activity cytokine activity chemokine activity heparin binding phospholipase activator activity receptor signaling protein tyrosine kinase activator activity CCR1 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity chemokine receptor binding protein homodimerization activity protein self-association chemokine receptor antagonist activity cytokine intercellular signal molecule
Process(es)
MAPK cascade positive regulation of defense response to virus by host calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response immune response leukocyte cell-cell adhesion G-protein coupled receptor signaling pathway cell-cell signaling response to toxic substance positive regulation of gene expression positive regulation of macrophage chemotaxis positive regulation of T cell chemotaxis positive regulation of phosphatidylinositol 3-kinase signaling positive regulation of smooth muscle cell migration positive regulation of cell migration pseudopodium assembly positive regulation of cellular biosynthetic process activation of phospholipase D activity positive regulation of fever generation lipopolysaccharide-mediated signaling pathway positive regulation of cell-cell adhesion mediated by integrin response to cytokine positive regulation of homotypic cell-cell adhesion response to interferon-gamma response to tumor necrosis factor T-helper 1 cell diapedesis positive regulation of T cell proliferation neutrophil activation positive regulation of phosphorylation positive regulation of tyrosine phosphorylation of STAT protein protein kinase B signaling cellular protein complex assembly negative regulation by host of viral transcription negative regulation of viral genome replication positive regulation of neuron differentiation positive regulation of osteoclast differentiation negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis positive regulation of cell adhesion eosinophil chemotaxis lymphocyte chemotaxis positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation regulation of insulin secretion regulation of T cell activation positive chemotaxis protein tetramerization positive regulation of calcium ion transport negative regulation of cell death positive regulation of mast cell chemotaxis positive regulation of protein tyrosine kinase activity chemokine-mediated signaling pathway negative regulation of chemokine-mediated signaling pathway negative regulation of T cell apoptotic process positive regulation of T cell apoptotic process cellular response to organic cyclic compound positive regulation of monocyte chemotaxis extracellular negative regulation of signal transduction regulation of neuron death negative regulation of neuron death negative regulation of macrophage apoptotic process negative regulation of receptor activity positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of T cell migration positive regulation of natural killer cell chemotaxis
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