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Invitrogen
CCL5 (RANTES) Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
CCL5 (RANTES) Antibody (PA5-47536) in Neu
Neutralization of CCL5 (RANTES) in BaF3 mouse pro‚B cell line transfected with human CCR5. Samples were incubated in CCL5 (RANTES) polyclonal antibody (Product # PA5-47536) using a dilution of 80 ng/mL. Recombinant Feline CCL5/RANTES chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Feline CCL5/RANTES is neutralized (green line) by increasing... View More
Product Details
PA5-47536
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat
Host/Isotype
Sheep
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant feline CCL5/RANTES Tyr26-Asn92 (Lys84Tyr and Gly85 del)
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606493
Product Specific Information
In sandwich ELISAs, less than 0.02% cross-reactivity with recombinant human RANTES, recombinant mouse RANTES, and recombinant cotton rat RANTES is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 5; MGC17164; OTTHUMP00000197106; SIS δ; SISδ; small inducible cytokine A5; Small-inducible cytokine A5; T-cell-specific protein RANTES
Gene Aliases: CCL5; RANTES; SCYA5
UniProt ID: (Cat) Q8SQ40
Entrez Gene ID: (Cat) 493689
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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