Invitrogen

CCL5 (RANTES) Monoclonal Antibody (VL-1), Biotin

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View all (33) CCL5 (RANTES) antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

M230B

Applications
Tested Dilution
Publications

Western Blot (WB)

Assay-dependent
-

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

VL-1

Immunogen

Recombinant human RANTES

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS with 4% BSA

Contains

proprietary preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223559

Product Specific Information

M230B targets RANTES in ELISA, and WB applications and shows reactivity with Human samples.

The M230B immunogen is recombinant human RANTES.

M230B detects RANTES which has a predicted molecular weight of approximately 8 kDa.

The M230B Rantes antibody (biotinylated conjugate of clone VL-1) has successfully been paired as the detection antibody in a sandwich ELISA with polyclonal capture antibody P230E. Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody M230B (clone VL-1) and antibody P230E have successfully been used in combination with recombinant Rantes protein SRNTS in ELISA applications.

Target Information

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; OTTHUMP00000197106; regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228

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Gene Aliases: CCL5; D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228

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UniProt ID: (Human) P13501

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Entrez Gene ID: (Human) 6352

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Function(s)
phosphatidylinositol phospholipase C activity protein kinase activity protein binding chemokine activity heparin binding phospholipase activator activity receptor signaling protein tyrosine kinase activator activity CCR1 chemokine receptor binding CCR4 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity chemokine receptor binding protein homodimerization activity protein self-association chemokine receptor antagonist activity cytokine intercellular signal molecule
Process(es)
MAPK cascade dendritic cell chemotaxis chronic inflammatory response monocyte chemotaxis regulation of chronic inflammatory response calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response leukocyte cell-cell adhesion G-protein coupled receptor signaling pathway cell-cell signaling aging response to virus response to toxic substance response to salt stress positive regulation of activation of JAK2 kinase activity positive regulation of macrophage chemotaxis positive regulation of T cell chemotaxis positive regulation of phosphatidylinositol 3-kinase signaling response to activity positive regulation of smooth muscle cell migration dibenzo-p-dioxin metabolic process positive regulation of cell migration neutrophil chemotaxis positive regulation of cellular biosynthetic process activation of phospholipase D activity positive regulation of fever generation lipopolysaccharide-mediated signaling pathway response to insulin positive regulation of cell-cell adhesion mediated by integrin positive regulation of homotypic cell-cell adhesion positive regulation of T cell proliferation neutrophil activation positive regulation of phosphorylation response to drug positive regulation of tyrosine phosphorylation of STAT protein protein kinase B signaling positive regulation of GTPase activity cellular protein complex assembly response to estrogen negative regulation by host of viral transcription cellular response to fibroblast growth factor stimulus positive regulation of viral genome replication negative regulation of viral genome replication positive regulation of innate immune response positive regulation of neuron differentiation positive regulation of osteoclast differentiation negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of translational initiation positive regulation of JAK-STAT cascade eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of inflammatory response regulation of insulin secretion regulation of T cell activation positive chemotaxis protein tetramerization response to glucocorticoid positive regulation of calcium ion transport positive regulation of mast cell chemotaxis positive regulation of protein tyrosine kinase activity chemokine-mediated signaling pathway negative regulation of chemokine-mediated signaling pathway negative regulation of T cell apoptotic process positive regulation of T cell apoptotic process positive regulation of ERK1 and ERK2 cascade response to cholesterol cellular response to amino acid stimulus cellular response to vitamin K cellular response to morphine cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to ethanol cellular response to high density lipoprotein particle stimulus cellular response to organic cyclic compound cellular response to alkyl hydroperoxide cellular response to transforming growth factor beta stimulus positive regulation of monocyte chemotaxis regulation of neuron death negative regulation of neuron death negative regulation of macrophage apoptotic process positive regulation of T cell migration positive regulation of natural killer cell chemotaxis
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