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CCL6 Antibody (500-P112BT-25UG) in WB
Western Blot: To detect mC10 by Western Blot analysis CCL6 Polyclonal Antibody, Biotin (Product # 500-P112BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mC-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P112BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine C10 (CCL6)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929267
Product Specific Information
AA Sequence of recombinant protein: GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine C10 (CCL6). Anti-Murine C10 (CCL6)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine C10 (CCL6) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine C10 (CCL6) (500-P112) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine C10 (CCL6).
Western Blot: To detect mC10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mC-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P112BT-1MG will be provided as 2 x 500 µg
Target Information
Chemokine(C-C motif) ligand 6(CCL6) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be greatly induced under conditions suitable for myeloid cell differentiation. It is highly expressed in bone marrow cultures that have been stimulated with the cytokine GM-CSF. Some low levels of gene expression also occur in certain cell lines of myeloid origin(e.g. the immature myeloid cell lines DA3 and 32D cl3, and the macrophage cell line P388D) that can also be greatly induced in culture with GM-CSF. However, in activated T cell lines, expression of CCL6 is greatly reduced. CCL6 can also be induced in the mouse lung by the cytokine interleukin 13. Mouse CCL6 is located on chromosome 11. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 6; CC chemokine C10; Protein C10; small inducible cytokine A6; Small-inducible cytokine A6
Gene Aliases: C10; Ccl6; MRP-1; Scya6
UniProt ID: (Mouse) P27784
Entrez Gene ID: (Mouse) 20305
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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