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CCR8 Antibody (PA5-96908) in WB
Western blot analysis of CCR8 in extracts of various cell lines. Samples were incubated with CCR8 Polyclonal antibody (Product # PA5-96908) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96908
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 296-355 of human CCR8 (NP_0051921)
Conjugate
Form
Liquid
Concentration
0.8 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2808710
Product Specific Information
Immunogen sequence: NPVIYAFVGE KFKKHLSEIF QKSCSQIFNY LGRQMPRESC EKSSSCQQHS SRSSSVDYIL; Positive Samples: Mouse brain, Rat brain; Cellular Location: Cell membrane, Multi-pass membrane protein
Target Information
CCR8 encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are important for the migration of various cell types into the inflammatory sites. This receptor protein preferentially expresses in the thymus. I-309, thymus activation-regulated cytokine (TARC) and macrophage inflammatory protein-1 beta (MIP1 beta) have been identified as ligands of this receptor. Studies of this receptor and its ligands suggested its role in regulation of monocyte chemotaxis and thymic cell apoptosis. More specifically, this receptor may contribute to the proper positioning of activated T cells within the antigenic challenge sites and specialized areas of lymphoid tissues. This gene is located at the chemokine receptor gene cluster region. CCR8 expression has been reported in spleen and thymus, natural-killer cells, monocytes, and T cells, and at lower levels in peripheral blood leukocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C chemokine receptor type 8; C-C CKR-8; C-C motif chemokine receptor 8; cd198; CDw198; chemokine (C-C motif) receptor 8; MGC129966; MGC129973
Gene Aliases: C-C; C-C CKR-8; CC-CKR-8; CCR-8; Ccr8; CKR-8; Cmkbr8; mCCR8; Ter1
UniProt ID: (Mouse) P56484
Entrez Gene ID: (Rat) 301066, (Mouse) 12776
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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