Proteintech

CCT8 Polyclonal Antibody

CCT8 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

CCT8 Antibody (12263-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol) fixed MCF-7 cells using 12263-1-AP (CCT8 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CCT8 Antibody in Immunoprecipitation (IP)

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Product Details

12263-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 11 publications 11 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CCT8 Fusion Protein Ag2926 (146-497 aa encoded by BC012584)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FNVDNIRVC KILGSGISSS SVLHGMVFKK ETEGDVTSVK DAKIAVYSCP FDGMITETKG TVLIKTAEEL MNFSKGEENL MDAQVKAIAD TGANVVVTGG KVADMALHYA NKYNIMLVRL NSKWDLRRLC KTVGATALPR LTPPVLEEMG HCDSVYLSEV GDTQVVVFKH EKEDGAISTI VLRGSTDNLM DDIERAVDDG VNTFKVLTRD KRLVPGGGAT EIELAKQITS YGETCPGLEQ YAIKKFAEAF EAIPRALAEN SGVKANEVIS KLYAVHQEGN KNVGLDIEAE VPAVKDMLEA GILDTYLGKY WAIKLATNAA VTVLRVDQII MAKPAGGPKP PSGKKDWDDD QND (146-497 aa encoded by BC012584)

Target Information

TCP-1 theta is theta subunit of the CCT (chaperonin containing TCP1 complex) chaperonin molecule which is abundant in the eukaryotic cytosol and may be involved in the transport and assembly of the newly synthesized proteins. Molecular chaperones assist in the protein folding upon ATP hydrolysis and TCP-1 theta may play a role in the assembly of the BBsome, a complex that is involved in ciliogenesis regulating transport vesicles to the cilia. Further, TCP-1 theta is also involved in the folding of actin and tubulin. The intact CCT complex is composed of eight polypeptides in a double ring structure. CCT is important within cells in the folding of the VHL tumor suppressor protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibiting de novo ceramide synthesis restores mitochondrial and protein homeostasis in muscle aging.

Science translational medicine

Lima TI,Laurila PP,Wohlwend M,Morel JD,Goeminne LJE,Li H,Romani M,Li X,Oh CM,Park D,Rodríguez-López S,Ivanisevic J,Gallart-Ayala H,Crisol B,Delort F,Batonnet-Pichon S,Silveira LR,Sankabattula Pavani Veera Venkata L,Padala AK,Jain S,Auwerx J

Wed May 17 00:00:00 EDT 2023

An Rtn4/Nogo-A-interacting micropeptide modulates synaptic plasticity with age.

PloS one

Kragness S,Clark Z,Mullin A,Guidry J,Earls LR

Mon Jul 04 00:00:00 EDT 2022

A cytosolic chaperone complex controls folding and degradation of type III CD38.

The Journal of biological chemistry

Wu Y,Zhang J,Fang L,Lee HC,Zhao YJ

Fri Mar 15 00:00:00 EDT 2019

Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.

Virology

Gopal S,Perez E,Xia AY,Knowlton JJ,Cerqueira F,Dermody TS,Upton JW

Sat Sep 01 00:00:00 EDT 2018

Murine cytomegalovirus M72 promotes acute virus replication in vivo and is a substrate of the TRiC/CCT complex.

Virology

Gopal S,Perez E,Xia AY,Knowlton JJ,Cerqueira F,Dermody TS,Upton JW

Sat Sep 01 00:00:00 EDT 2018

Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan.

Nature communications

Noormohammadi A,Khodakarami A,Gutierrez-Garcia R,Lee HJ,Koyuncu S,König T,Schindler C,Saez I,Fatima A,Dieterich C,Vilchez D

Mon Nov 28 00:00:00 EST 2016

Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan.

Nature communications

Noormohammadi A,Khodakarami A,Gutierrez-Garcia R,Lee HJ,Koyuncu S,König T,Schindler C,Saez I,Fatima A,Dieterich C,Vilchez D

Mon Nov 28 00:00:00 EST 2016

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

Nature genetics

Tarkar A,Loges NT,Slagle CE,Francis R,Dougherty GW,Tamayo JV,Shook B,Cantino M,Schwartz D,Jahnke C,Olbrich H,Werner C,Raidt J,Pennekamp P,Abouhamed M,Hjeij R,Köhler G,Griese M,Li Y,Lemke K,Klena N,Liu X,Gabriel G,Tobita K,Jaspers M,Morgan LC,Shapiro AJ,Letteboer SJ,Mans DA,Carson JL,Leigh MW,Wolf WE,Chen S,Lucas JS,Onoufriadis A,Plagnol V,Schmidts M,Boldt K,Roepman R,Zariwala MA,Lo CW,Mitchison HM,Knowles MR,Burdine RD,Loturco JJ,Omran H

Sun Sep 01 00:00:00 EDT 2013

DYX1C1 is required for axonemal dynein assembly and ciliary motility.

Nature genetics

Sun Sep 01 00:00:00 EDT 2013

Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.

Pathogens and disease

Hirai K,Maeda H,Omori K,Yamamoto T,Kokeguchi S,Takashiba S

Sat Jun 01 00:00:00 EDT 2013

Serum antibody response to group II chaperonin from Methanobrevibacter oralis and human chaperonin CCT.

Pathogens and disease

Hirai K,Maeda H,Omori K,Yamamoto T,Kokeguchi S,Takashiba S

Sat Jun 01 00:00:00 EDT 2013

Network organization of the huntingtin proteomic interactome in mammalian brain.

Neuron

Shirasaki DI,Greiner ER,Al-Ramahi I,Gray M,Boontheung P,Geschwind DH,Botas J,Coppola G,Horvath S,Loo JA,Yang XW

Thu Jul 12 00:00:00 EDT 2012

Network organization of the huntingtin proteomic interactome in mammalian brain.

Neuron

Shirasaki DI,Greiner ER,Al-Ramahi I,Gray M,Boontheung P,Geschwind DH,Botas J,Coppola G,Horvath S,Loo JA,Yang XW

Thu Jul 12 00:00:00 EDT 2012

New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells.

Proteomics. Clinical applications

Huang R,Yu M,Li CY,Zhan YQ,Xu WX,Xu F,Ge CH,Li W,Yang XM

Mon Oct 01 00:00:00 EDT 2012

New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells.

Proteomics. Clinical applications

Huang R,Yu M,Li CY,Zhan YQ,Xu WX,Xu F,Ge CH,Li W,Yang XM

Mon Oct 01 00:00:00 EDT 2012

TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.

PloS one

Somalinga BR,Day CE,Wei S,Roth MG,Thomas PJ

Mon Sep 17 00:00:00 EDT 2012

TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.

PloS one

Somalinga BR,Day CE,Wei S,Roth MG,Thomas PJ

Mon Sep 17 00:00:00 EDT 2012

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Proceedings of the National Academy of Sciences of the United States of America

Seo S,Baye LM,Schulz NP,Beck JS,Zhang Q,Slusarski DC,Sheffield VC

Tue Jan 26 00:00:00 EST 2010

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Proceedings of the National Academy of Sciences of the United States of America

Seo S,Baye LM,Schulz NP,Beck JS,Zhang Q,Slusarski DC,Sheffield VC

Tue Jan 26 00:00:00 EST 2010

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Proceedings of the National Academy of Sciences of the United States of America

Seo S,Baye LM,Schulz NP,Beck JS,Zhang Q,Slusarski DC,Sheffield VC

Tue Jan 26 00:00:00 EST 2010

BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.

Proceedings of the National Academy of Sciences of the United States of America

Seo S,Baye LM,Schulz NP,Beck JS,Zhang Q,Slusarski DC,Sheffield VC

Tue Jan 26 00:00:00 EST 2010

Antigenic group II chaperonin in Methanobrevibacter oralis may cross-react with human chaperonin CCT.

Molecular oral microbiology

Yamabe K,Maeda H,Kokeguchi S,Soga Y,Meguro M,Naruishi K,Asakawa S,Takashiba S

Thu Apr 01 00:00:00 EDT 2010

Antigenic group II chaperonin in Methanobrevibacter oralis may cross-react with human chaperonin CCT.

Molecular oral microbiology

Yamabe K,Maeda H,Kokeguchi S,Soga Y,Meguro M,Naruishi K,Asakawa S,Takashiba S

Thu Apr 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: CCT-theta; Chaperonin containing T-complex polypeptide 1 subunit 8; chaperonin containing TCP1, subunit 8 (theta); chaperonin subunit 8 (theta); Renal carcinoma antigen NY-REN-15; T-complex protein 1 subunit theta; TCP-1-theta

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Gene Aliases: AI132397; C21orf112; CCT8; CCTQ; D21S246; KIAA0002; PRED71; Tcpq

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UniProt ID: (Human) P50990, (Mouse) P42932

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Entrez Gene ID: (Human) 10694, (Rat) 288305, (Mouse) 12469

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Function(s)

protein binding ATP binding ATPase activity, coupled unfolded protein binding cadherin binding involved in cell-cell adhesion nucleotide binding chaperonin

Process(es)

protein folding binding of sperm to zona pellucida positive regulation of telomere maintenance via telomerase pore complex assembly protein stabilization cell-cell adhesion toxin transport positive regulation of establishment of protein localization to telomere positive regulation of protein localization to Cajal body positive regulation of telomerase RNA localization to Cajal body

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CCT8 Polyclonal Antibody

CCT8 Antibody (12263-1-AP) in ICC/IF

Product Details

12263-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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