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Invitrogen
CD10 Recombinant Rabbit Monoclonal Antibody (6P8G4)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 3
CD10 Antibody (MA5-35196) in WB
Western blot analysis of CD10 in extracts of various cell lines. Samples were incubated with CD10 Monoclonal antibody (Product # MA5-35196) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
MA5-35196
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
6P8G4
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 52-750 of human CD10/MME (NP_0008932)
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2849100
Product Specific Information
Immunogen sequence: YDDGICKSSD CIKSAARLIQ NMDATTEPCT DFFKYACGGW LKRNVIPETS SRYGNFDILR DELEVVLKDV LQEPKTEDIV AVQKAKALYR SCINESAIDS RGGEPLLKLL PDIYGWPVAT ENWEQKYGAS WTAEKAIAQL NSKYGKKVLI NLFVGTDDKN SVNHVIHIDQ PRLGLPSRDY YECTGIYKEA CTAYVDFMIS VARLIRQEER LPIDENQLAL EMNKVMELEK EIANATAKPE DRNDPMLLYN KMTLAQIQNN FSLEINGKPF SWLNFTNEIM STVNISITNE EDVVVYAPEY LTKLKPILTK YSARDLQNLM SWRFIMDLVS SLSRTYKESR NAFRKALYGT TSETATWRRC ANYVNGNMEN AVGRLYVEAA FAGESKHVVE DLIAQIREVF IQTLDDLTWM DAETKKRAEE KALAIKERIG YPDDIVSNDN KLNNEYLELN YKEDEYFENI IQNLKFSQSK QLKKLREKVD KDEWISGAAV VNAFYSSGRN QIVFPAGILQ PPFFSAQQSN SLNYGGIGMV IGHEITHGFD DNGRNFNKDG DLVDWWTQQS ASNFKEQSQC MVYQYGNFSW DLAGGQHLNG INTLGENIAD NGGLGQAYRA YQNYIKKNGE EKLLPGLDLN HKQLFFLNFA QVWCGTYRPE YAVNSIKTDV HSPGNFRIIG TLQNSAEFSE AFHCRKNSYM NPEKKCRVW
Target Information
CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. CD10 is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. Further, CD10 is a 100 kDa type II transmembrane glycoprotein that exists in a single copy of greater than 45 kb. The 5' untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts and the coding region is not affected by alternative splicing. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie tooth Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Atriopeptidase; CALLA; CD10; common acute lymphoblastic leukemia antigen; Common acute lymphocytic leukemia antigen; Enkephalinase; membrane metallo endopeptidase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase (neutral endopeptidase/enkephalinase); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; NEP; Neprilysin; neprilysin-390; neprilysin-411; Neutral endopeptidase 24.11; SFE; Skin fibroblast elastase
Gene Aliases: 6030454K05Rik; C85356; CALLA; CD10; CMT2T; EPN; MME; NEP; SFE
UniProt ID: (Human) P08473, (Mouse) Q61391, (Rat) P07861
Entrez Gene ID: (Human) 4311, (Mouse) 17380, (Rat) 24590
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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