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Invitrogen
CD10 Recombinant Rabbit Monoclonal Antibody (RM337)
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 9
CD10 Antibody (MA5-27893) in ICC/IF
Immunofluorescence analysis of CD10 was performed using LNCaP and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD10 Rabbit Monoclonal Antibody (Product # MA5-27893) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) at a dilution of... View More
Product Details
MA5-27893
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM337
Immunogen
A peptide corresponding to the N-terminus of human CD10
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2744947
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. CD10 is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. Further, CD10 is a 100 kDa type II transmembrane glycoprotein that exists in a single copy of greater than 45 kb. The 5' untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts and the coding region is not affected by alternative splicing. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie tooth Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Atriopeptidase; CALLA; CD10; common acute lymphoblastic leukemia antigen; Common acute lymphocytic leukemia antigen; Enkephalinase; membrane metallo endopeptidase; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10); membrane metallo-endopeptidase (neutral endopeptidase/enkephalinase); membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; NEP; Neprilysin; neprilysin-390; neprilysin-411; Neutral endopeptidase 24.11; SFE; Skin fibroblast elastase
Gene Aliases: 6030454K05Rik; C85356; CALLA; CD10; CMT2T; EPN; MME; NEP; SFE
UniProt ID: (Human) P08473, (Mouse) Q61391, (Rat) P07861
Entrez Gene ID: (Human) 4311, (Mouse) 17380, (Rat) 24590
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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