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Invitrogen
CD102 (ICAM-2) Monoclonal Antibody (CBR-IC2/2), eBioscience™
FIGURE: 1 / 2
CD102 (ICAM-2) Antibody (14-1029-82) in ICC/IF
Immunofluorescence analysis of CD102 (ICAM-2) was performed using log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD102 (ICAM-2) Mouse Monoclonal Antibody (CBR-IC2/2) (Product # 14-1029-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degre... View More
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-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD102 (ICAM-2) Antibody (14-1029-82) in Flow")
Product Details
14-1029-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
CBR-IC2/2
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_953553
Product Specific Information
Description: The CBRIC2/2 monoclonal antibody reacts with human ICAM-2 (CD102). ICAM-2 is a 55-65 kD transmembrane glycoprotein possessing 2 extracellular Ig domains, a single transmembrane domain, and a short 26-amino acid cytoplasmic domain. ICAM-2 is expressed on most leukocytes, and is strongly expressed on vascular endothelial cells. ICAM-2 is a ligand of integrin CD11a/CD18 (LFA-1), and also has been reported to bind integrin CD11b/CD18 (Mac-1). ICAM-2 is an intercellular adhesion molecule involved in lymphocyte homing to sites of inflammation. The CBRIC2/2 antibody has been reported to have blocking activity.
Applications Reported: This CBRIC2/2 antibody has been reported for use in flow cytometric analysis, immunoblotting, immunoprecipitation, immunohistochemical staining, and functional assays.
Applications Tested: This CBRIC2/2 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The CBRIC2/2 antibody has also been found useful for immunoblotting of a human peripheral blood cell lysate, recognizing a protein of approximately 55 kD under non-reducing conditions. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ICAM-2 is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. ICAM-2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. Diseases associated with ICAM2 include Colon Carcinoma In Situ.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD102; ICAM-2; Intercellular adhesion molecule 2; RP23-418O11.10-001
Gene Aliases: CD102; ICAM2
UniProt ID: (Human) P13598
Entrez Gene ID: (Human) 3384
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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