Invitrogen

CD105 (Endoglin) Monoclonal Antibody (MJ7/18), PE-Cyanine7, eBioscience™

18 Published Figures

23 References

View all (127) CD105 (Endoglin) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (18)

CD105 (Endoglin) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 19

CD105 (Endoglin) Antibody (25-1051-82) in Flow

Staining of bEnd-3 cell line with 0.125 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (blue histogram) or 0.125 µg of Anti-Mouse CD105 (Endoglin) PE-Cyanine7 (purple histogram). Total viable cells were used for analysis.

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Product Details

25-1051-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MJ7/18

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573380

Product Specific Information

Description: The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin. This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported: This MJ7/18 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MJ7/18 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Overexpression of fibroblast growth factor receptor 2 in bone marrow mesenchymal stem cells enhances osteogenesis and promotes critical cranial bone defect regeneration.

Frontiers in cell and developmental biology

Zhou Y,Zhu P,Shen S,Wang Y,Li B,Guo B,Li H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Sun Jun 04 00:00:00 EDT 2023

Fetal Muse-based therapy prevents lethal radio-induced gastrointestinal syndrome by intestinal regeneration.

Stem cell research & therapy

Dushime H,Moreno SG,Linard C,Adrait A,Couté Y,Peltzer J,Messiaen S,Torres C,Bensemmane L,Lewandowski D,Romeo PH,Petit V,Gault N

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Fri Aug 11 00:00:00 EDT 2023

Frontiers in cellular neuroscience

Kyryk V,Tsupykov O,Ustymenko A,Smozhanik E,Govbakh I,Butenko G,Skibo G

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Tue Mar 07 00:00:00 EST 2023

Prenatal inflammation perturbs murine fetal hematopoietic development and causes persistent changes to postnatal immunity.

Cell reports

López DA,Apostol AC,Lebish EJ,Valencia CH,Romero-Mulero MC,Pavlovich PV,Hernandez GE,Forsberg EC,Cabezas-Wallscheid N,Beaudin AE

25-1051-82 was used in Flow cytometry/Cell sorting to show that murine fetal HSPCs respond to prenatal inflammation in utero and that the fetal response shapes postnatal hematopoiesis and immune cell function.

Tue Nov 22 00:00:00 EST 2022

A somatic mutation in moesin drives progression into acute myeloid leukemia.

Science advances

Yuan O,Ugale A,de Marchi T,Anthonydhason V,Konturek-Ciesla A,Wan H,Eldeeb M,Drabe C,Jassinskaja M,Hansson J,Hidalgo I,Velasco-Hernandez T,Cammenga J,Magee JA,Niméus E,Bryder D

25-1051-82 was used in Flow cytometry/Cell sorting to demonstrate a critical role of ERM proteins in AML, with implications also for human cancer.

Fri Apr 22 00:00:00 EDT 2022

Induction of mesenchymal stem cell‑like transformation in rat primary glial cells using hypoxia, mild hypothermia and growth factors.

Molecular medicine reports

Wei H,Zhou W,Hu G,Shi C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Extracellular vesicles from recombinant cell factories improve the activity and efficacy of enzymes defective in lysosomal storage disorders.

Journal of extracellular vesicles

Seras-Franzoso J,Díaz-Riascos ZV,Corchero JL,González P,García-Aranda N,Mandaña M,Riera R,Boullosa A,Mancilla S,Grayston A,Moltó-Abad M,Garcia-Fruitós E,Mendoza R,Pintos-Morell G,Albertazzi L,Rosell A,Casas J,Villaverde A,Schwartz S,Abasolo I

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Extracellular HMGB-1 activates inflammatory signaling in tendon cells and tissues.

Therapeutic advances in chronic disease

Zhang C,Gu X,Zhao G,Wang W,Shao J,Zhu J,Yuan T,Sun J,Nie D,Zhou Y

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Sun Apr 17 00:00:00 EDT 2022

Targeting compensatory MEK/ERK activation increases JAK inhibitor efficacy in myeloproliferative neoplasms.

The Journal of clinical investigation

Stivala S,Codilupi T,Brkic S,Baerenwaldt A,Ghosh N,Hao-Shen H,Dirnhofer S,Dettmer MS,Simillion C,Kaufmann BA,Chiu S,Keller M,Kleppe M,Hilpert M,Buser AS,Passweg JR,Radimerski T,Skoda RC,Levine RL,Meyer SC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Mon Mar 04 00:00:00 EST 2019

LPS‑induced upregulation of the TLR4 signaling pathway inhibits osteogenic differentiation of human periodontal ligament stem cells under inflammatory conditions.

International journal of molecular medicine

Yu B,Li Q,Zhou M

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

The microRNAs miR-204 and miR-211 maintain joint homeostasis and protect against osteoarthritis progression.

Nature communications

Huang J,Zhao L,Fan Y,Liao L,Ma PX,Xiao G,Chen D

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Fri Jun 28 00:00:00 EDT 2019

Isolation and functional assessment of mouse skeletal stem cell lineage.

Nature protocols

Gulati GS,Murphy MP,Marecic O,Lopez M,Brewer RE,Koepke LS,Manjunath A,Ransom RC,Salhotra A,Weissman IL,Longaker MT,Chan CKF

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells.

The Journal of biological chemistry

Ge J,Burnier L,Adamopoulou M,Kwa MQ,Schaks M,Rottner K,Brakebusch C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Fri Jun 15 00:00:00 EDT 2018

Cardiosphere-Derived Cells Require Endoglin for Paracrine-Mediated Angiogenesis.

Stem cell reports

Redgrave RE,Tual-Chalot S,Davison BJ,Singh E,Hall D,Amirrasouli MM,Gilchrist D,Medvinsky A,Arthur HM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Tue May 09 00:00:00 EDT 2017

Mice Lacking Endoglin in Macrophages Show an Impaired Immune Response.

PLoS genetics

Ojeda-Fernández L,Recio-Poveda L,Aristorena M,Lastres P,Blanco FJ,Sanz-Rodríguez F,Gallardo-Vara E,de las Casas-Engel M,Corbí Á,Arthur HM,Bernabeu C,Botella LM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2016

KLF4-dependent phenotypic modulation of smooth muscle cells has a key role in atherosclerotic plaque pathogenesis.

Nature medicine

Shankman LS,Gomez D,Cherepanova OA,Salmon M,Alencar GF,Haskins RM,Swiatlowska P,Newman AA,Greene ES,Straub AC,Isakson B,Randolph GJ,Owens GK

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Cell-autonomous heparanase modulates self-renewal and migration in bone marrow-derived mesenchymal stem cells.

Journal of biomedical science

Cheng CC,Lee YH,Lin SP,Huangfu WC,Liu IH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

Isolation and characterization of novel murine epiphysis derived mesenchymal stem cells.

PloS one

Cheng CC,Lian WS,Hsiao FS,Liu IH,Lin SP,Lee YH,Chang CC,Xiao GY,Huang HY,Cheng CF,Cheng WT,Wu SC

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2012

Adipose-derived stem cells promote lymphangiogenesis in response to VEGF-C stimulation or TGF-β1 inhibition.

Future oncology (London, England)

Yan A,Avraham T,Zampell JC,Haviv YS,Weitman E,Mehrara BJ

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP-Chip.

Biological procedures online

Weishaupt H,Attema JL

25-1051 was used in Flow cytometry/Cell sorting to document the genome-wide chromatin states of low abundant populations that comprise hematopoietic stem cells.

Sat May 15 00:00:00 EDT 2010

Circadian mechanisms in murine and human bone marrow mesenchymal stem cells following dexamethasone exposure.

Bone

Wu X,Yu G,Parks H,Hebert T,Goh BC,Dietrich MA,Pelled G,Izadpanah R,Gazit D,Bunnell BA,Gimble JM

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 25-1051-82) in Flow Cytometry

Thu May 01 00:00:00 EDT 2008

Monitoring response to anticancer therapy by targeting microbubbles to tumor vasculature.

Clinical cancer research : an official journal of the American Association for Cancer Research

Korpanty G,Carbon JG,Grayburn PA,Fleming JB,Brekken RA

Mon Jan 01 00:00:00 EST 2007

Liver sinusoidal endothelial cells tolerize T cells across MHC barriers in mice.

Journal of immunology (Baltimore, Md. : 1950)

Onoe T,Ohdan H,Tokita D,Shishida M,Tanaka Y,Hara H,Zhou W,Ishiyama K,Mitsuta H,Ide K,Asahara T

Fri Jul 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD105; Cell surface MJ7/18 antigen; Endoglin; ORW; transmembrane glycoprotein

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Gene Aliases: AI528660; AI662476; CD105; Edg; Endo; Eng; S-endoglin

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UniProt ID: (Mouse) Q63961

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Entrez Gene ID: (Mouse) 13805

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Function(s)

transforming growth factor beta-activated receptor activity transforming growth factor beta receptor, cytoplasmic mediator activity type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding type I transforming growth factor beta receptor binding BMP binding protein homodimerization activity transforming growth factor beta binding transmembrane signal receptor

Process(es)

negative regulation of transcription from RNA polymerase II promoter angiogenesis patterning of blood vessels vasculogenesis response to hypoxia positive regulation of protein phosphorylation negative regulation of endothelial cell proliferation heart looping positive regulation of systemic arterial blood pressure cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated protein phosphorylation cell adhesion transmembrane receptor protein serine/threonine kinase signaling pathway transforming growth factor beta receptor signaling pathway heart development positive regulation of gene expression positive regulation of pathway-restricted SMAD protein phosphorylation cell migration regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis extracellular matrix disassembly negative regulation of cell migration positive regulation of BMP signaling pathway negative regulation of protein autophosphorylation positive regulation of collagen biosynthetic process intracellular signal transduction wound healing positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis cell motility negative regulation of nitric-oxide synthase activity cell chemotaxis negative regulation of pathway-restricted SMAD protein phosphorylation extracellular matrix constituent secretion detection of hypoxia

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CD105 (Endoglin) Monoclonal Antibody (MJ7/18), PE-Cyanine7, eBioscience™

CD105 (Endoglin) Antibody (25-1051-82) in Flow

Product Details

25-1051-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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